Application Note #144
Kinetic evaluation of indomethacin binding. Different concentrations of analyte (coloured curves) and fit (black curves).
The interaction of a small molecular weight drug (indomethacin) to Human serum albumin (HSA) was measured with label-free Multi-Parametric Surface Plasmon Resonance (MP-SPR). HSA was attached to a sensor surface with amine coupling chemistry. Affinity (KD) and kinetic constants (ka and kd) of indomethacin interaction were determined with TraceDrawer™ for MP-SPR Navi™. Premium quality kinetic data was ensured using PureKinetics™ feature that separates bulk effect from binding kinetics.
Recommended instrument for this application
- If you are interested in reading more about small molecules, have a look at AN#155 about faster interaction measurements using KineticTitration and AN#137 about cell interactions.
- Do you want to find out how to calculate thickness out of MP-SPR measurements? Click here
- Do you want to see how MP-SPR instruments work? Click here
- Do you want to see MP-SPR instruments comparison? Click here