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Supported lipid layers characterization with MP-SPR

Application Note #139


Change in SPR peak minimum angle during lipid deposition, From left to right: SLB formation on SiO2 surface, SLV formation on PEG-SAM surface and SLB formation on 6 kDa dextran surface.

Multi-Parametric Surface Plasmon Resonance (MP-SPR) was used to characterize lipid layer structures adsorbed on a different substrates. Silicon dioxide and low molecule weight dextran surfaces supported formation of lipid bilayer (SLB) whereas thiolated polyethylene glycol supported vesicular layer formation (SLV). Thickness and refractive index of deposited lipid layer was calculated: SLB was about 5 nm thick layer whereas SLV was 10 nm thick layer.

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Further reading

  • If you are interested in lipids, we recommend to have a look at AN#157 about detection of toxin induced pore formation in membranes and AN#151 about measurement of soft and hard corona on liposomes.
  • Do you want to see how MP-SPR instruments work? Click here
  • Do you want to see comparison of MP-SPR instruments? Click here