Run biomolecular interaction measurements in half the time required for the traditional multi-cycle method without the need for regeneration
In the traditional multi-cycle titration method (figure below, lower panel), analyte samples are injected over the ligand surface at different concentrations. After sample association the analyte is allowed to dissociate from the surface by washing with buffer. Before injecting the next analyte concentration, the remaining bound analyte is removed injecting a suitable regeneration solution. Time-consuming assay optimization may be needed to find a suitable regeneration solution.
KineticTitration (figure, upper panel) removes the need for regeneration and significantly reduces the time required to run a full biomolecular interaction experiment, depending on assay type and number of concentrations up to half the time. In a typical KineticTitration procedure, analyte samples are injected over the surface in a series from low to high concentration without dissociation and regeneration steps between the sample injections. The dissociation rate is measured after the last analyte sample .
When is KineticTitration useful?
KineticTitration is suitable for all biomolecular interaction measurements. It is especially useful when:
- surface is difficult to regenerate
- regeneration is detrimental to the ligand
- less time is available for assay development