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Rapid and sensitive detection of PD-L1 exosomes using Cu-TCPP 2D MOF as a SPR sensitizer

Publication year: 2022
Authors: Wang Y. a, Mao Z. b, Chen Q. c, Koh K. d, Hu X. a **, Chen H. a *
Affiliations:

a – Center of Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, P.R. China

b – School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, P.R. China

c – School of Medicine, Shanghai University, Shanghai 200444, P.R. China

d – Institute of General Education, Pusan National University, Pusan 609-735, Republic of Korea

 

 

Published in: Biosensors and Bioelectronics, 201, 113954
doi: https://doi.org/10.1016/j.bios.2021.113954

Two-dimensional metal organic framework (2D MOF Cu-TCPP) with significantly enhanced photoelectric properties was synthesized by a simple hydrothermal method. The π-stacked electroactive porphyrin molecules of TCPP-based 2D MOF carry out charge transport in the MOF structure. The d-d band transition of Cu2+ and its 2D ultra-thin characteristics can produce excellent near-infrared light absorption to couple with SPR. Three key parameters including the refractive index sensitivity, detection accuracy and quality factor were improved significantly for 2D MOF modified gold chips. Especially, the refractive index sensitivity was increased from 98 to 137.67°/RIU after modified with 2D MOF. Thus, for the first time, we applied it as a signal enhancer to improve direct SPR assay for the Programmed death ligand-1 (PD-L1) exosomes. Owning to its large specific surface area, excellent photoelectric properties, highly ordered structure, good dispersion and biocompatibility, the LOD of the SPR sensor was 16.7 particles/mL. The reliability and practicability were further validated by analysis of PD-L1 exosomes in human serum samples. The recovery rate was 93.43 %–102.35%, with RSD of 5.79 %–14.6%. Given their excellent signal amplification ability, 2D MOF Cu-TCPP could serve as an ideal SPR sensitizer for rapid and sensitive detection of trace disease markers.