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Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance

Publication year: 2022
Authors: Norling K. a, Sjöberg M. a, Bally M b., Zhdanov V.P. a,c , Parveen N.* a, Höök F* a.

a – Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, 412 96
Gothenburg, Sweden

b – Department of Clinical Microbiology and Wallenberg Centre for Molecular Medicine, Umeå University,
901 85 Umeå, Sweden

c – Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Published in: Langmuir 2022 38 (8), 2550-2560
doi: DOI: 10.1021/acs.langmuir.1c03096

The mechanical properties of biological nanoparticles play a crucial role in their interaction with the cellular membrane, in particular for cellular uptake. This has significant implications for the design of pharmaceutical carrier particles. In this context, liposomes have become increasingly popular, among other reasons due to their customizability and easily varied physicochemical properties. With currently available methods, it is, however, not trivial to characterize the mechanical properties of nanoscopic liposomes especially with respect to the level of deformation induced upon their ligand− receptor-mediated interaction with laterally fluid cellular membranes. Here, we utilize the sensitivity of dual-wavelength surface plasmon resonance to probe the size and shape of bound liposomes (∼100 nm in diameter) as a means to quantify receptor-induced deformation during their interaction with a supported cell membrane mimic. By comparing biotinylated liposomes in gel and fluid phases, we demonstrate that fluid-phase liposomes are more prone to deformation than their gel-phase counterparts upon binding to the cell membrane mimic and that, as expected, the degree of deformation depends on the number of ligand−receptor pairs that are engaged in the multivalent binding.