PEDOT-polyamine composite films for bioelectrochemical platforms -flexible and easy to derivatize

Publication year: 2020
Authors: Sappia L. +a, Piccinini E. +a, von Binderling C. a, Knoll W. bcd, Marmisollé W. *a, Azzorini O. *acd

a- Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, CC 16 Suc. 4, La Plata, BI904DPI, Argentina

b - CEST, Competence Center for Electrochemical Surface Technologies, Konrad Lorenz Strasse 24, 3430 Tulln, Austria

c - Austrian Institute of Technology, Donau-City-Strasse 1, 1220 Vienna, Austria

d - CEST UNLP Partner Lab for Bioelectronics, Diagonal 64 y 113, La Plata (1900), Argentina

*- correcponding authors Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata - CONICET, Diagonal 64 y 113, La Plata (1900), Argentina

+ These authors contributed equally to this work

Published in: Material Sciece and Engineering: C, 2020, Vol. 109, p.110575
doi: 10.1016/j.msec.2019.110575

We report a straightforward route for the preparation of flexible, electrochemically stable and easily functionalizable poly(3,4-ethylenedioxythiophene) (PEDOT) composite films deposited on PET foils as biosensing platforms. For this purpose, poly(allylamine) hydrochloride (PAH) was blended with PEDOT to provide amine-bearing sites for further biofunctionalization as well as to improve the mechanical properties of the films. The conducting PEDOT-PAH composite films were characterized by cyclic voltammetry, UV–vis and Raman spectroscopies. An exhaustive stability study was carried out from the mechanical, morphological and electrochemical viewpoint. Subsequent sugar functionalization of the available amine groups from PAH allowed for the specific recognition of lectins and the subsequent self-assembly of glycoenzymes (glucose oxidase and horseradish peroxidase) concomitant with the prevention of non-specific protein fouling. The platforms presented good bioelectrochemical performance (glucose oxidation and hydrogen peroxide reduction) in the presence of redox mediators. The developed composite films constitute a promising option for the construction of all-polymer biosensing platforms with great potential owing to their flexibility, high transmittance, electrochemical stability and the possibility of glycosylation, which provides a simple route for specific biofunctionalization as well as an effective antifouling strategy

MP-SPR keywords: adsorption, lectin, protein, stability, surface coverage