Surfactant Interactions with Protein-Coated Surfaces: Comparison between Colloidal and Macroscopically Flat Surfaces

Publication year: 2020
Authors: Mateos H. 1,*, Valentini A. 2, Lopez F. 3, Palazzo G. 4
Affiliations:
1 - CSGI (Center for Colloid and Surface Science), via Orabona 4, 70125 Bari, Italy
2 - School of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK
3 - Department of Agricultural, Environmental and Food Sciences (DiAAA) and CSGI, University of Molise, Via De Sanctis, 86100 Campobasso, Italy
4 - Department of Chemistry, University of Bari, via Orabona 4, 70125 Bari, Italy
Published in: Biomimetics 2020, Vol. 5(3), p. 31
doi: 10.3390/biomimetics5030031

Surface interactions with polymers or proteins are extensively studied in a range of industrial and biomedical applications to control surface modification, cleaning, or biofilm formation. In this study we compare surfactant interactions with protein-coated silica surfaces differing in the degree of curvature (macroscopically flat and colloidal nanometric spheres). The interaction with a flat surface was probed by means of surface plasmon resonance (SPR) while dynamic light scattering (DLS) was used to study the interaction with colloidal SiO2 (radius 15 nm). First, the adsorption of bovine serum albumin (BSA) with both SiO2 surfaces to create a monolayer of coating protein was studied. Subsequently, the interaction of these BSA-coated surfaces with a non-ionic surfactant (a decanol ethoxylated with an average number of eight ethoxy groups) was investigated. A fair comparison between the results obtained by these two techniques on different geometries required the correction of SPR data for bound water and DLS results for particle curvature. Thus, the treated data have excellent quantitative agreement independently of the geometry of the surface suggesting the formation of multilayers of C10PEG over the protein coating. The results also show a marked different affinity of the surfactant towards BSA when the protein is deposited on a flat surface or individually dissolved in solution.


MP-SPR keywords: protein adsorption, SiO2 sensor slide, surface coverage, surfactant interaction