Kinetic and thermodynamic study of bovine serum albumin interaction with rifampicin using surface plasmon resonance and molecular docking methods

Publication year: 2017
Authors: Sharifi M. 1, Dolatabadi J.E. 2, Fathi F. 3, Rashidi M. 2, Jafari B. 4, Tajalli H. 1, Rashidi M.R. 2
Affiliations:

1 - University of Tabriz, Research Institute for Applied Physics and Astronomy, Tabriz, Iran
2 - Tabriz University of Medical Sciences, Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz, Iran
3 - Tabriz University of Medical Sciences, Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz,
Tabriz University of Medical Sciences, Student Research Committee, Tabriz, Iran
4 - Tabriz University of Medical Sciences, Biotechnology Research Center, Tabriz, Iran

Published in: Journal of Biomedical Optics, 2017, Vol. 22(3), p.
doi: 10.1117/1.JBO.22.3.037002

The interaction of bovine serum albumin (BSA) with various drugs, such as antibiotics, due to the importance of BSA in drug delivery has attracted increasing research attention at present. Therefore, the aim of this study was investigation of BSA interaction with rifampicin using surface plasmon resonance (SPR) and molecular docking methods under the imitated physiological conditions (pH=7.4pH=7.4). BSA immobilization on carboxymethyl dextran hydrogel chip has been carried out after activation with N-hydroxysuccinimide/N-ethyl-N-(3-diethylaminopropyl) carbodiimide. The dose-response sensorgrams of BSA upon increasing concentration of refampicin were attained in SPR analysis. The high affinity of rifampicin to BSA was demonstrated by a low equilibrium constants (KDKD) value (3.46×1053.46×10−5 at 40°C). The process of kinetic values changing shows that affinity of BSA to rifampicin decreased with rising temperature. The positive value of both enthalpy change (ΔHΔH) and entropy change (ΔSΔS) showed that hydrophobic force plays major role in the BSA interaction with rifampicin. The positive value of ΔGΔG was indicative of nonspontaneous and enthalpy-driven binding process. In addition, according to the molecular docking study, hydrogen binding has some contributions in the interaction of rifampicin with BSA.


MP-SPR keywords: affinity, antibiotic, binding, BSA, different temperatures (25 C – 40 C), drug, enthalpy, entropy, interaction mechanism, kinetics, protein, rifampicin, small molecules