Fluid dynamics modeling for synchronizing surface plasmon resonance and quartz crystal microbalance as tools for biomolecular and targeted drug delivery studies

Publication year: 2012
Authors: Tapani Viitala (a); Huamin Liang (a); Mayur Gupta (a), (b); Thomas Zwinger (c); Marjo Yliperttula (a); Alex Bunker (d); (e); ⇑

a) Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, P.O.Box 56, FI-00014 University of Helsinki, Finland 
b) Mechanical Engineering Department, Indian Institute of Technology, 721302 Kharagpur, Indiac 
c) CSC–IT Center for Science Ltd., P.O.Box 405, FI-02101 Espoo, Finland
d) Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P.O.Box 56, FI-00014 University of Helsinki, Finland
e) Department of Chemistry, Aalto University, P.O.Box 16100, FI-00076 Aalto, Finland

Published in: Journal of Colloid and Interface Science, Vol. 378 (2012), p. 251–259
doi: 10.1016/j.jcis.2012.04.012

We have used computational fluid dynamics modeling (CFD) to synchronize the flow conditions in the flow channels of two complementary surface-sensitive characterization techniques: surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). Since the footprint of the flow channels of the two devices is specified by their function, the flow behavior can only be varied either by altering the height ofthe flow channel, or altering the volumetric rate of flow (flow rate) through the channel. The relevant quantity that must be calibrated is the shear strain on the measurement surface (center and bottom) of the flow channel. Our CFD modeling shows that the flow behavior is in the Stokes flow regime. We were thus able to generate a scaling expression with parameters for flow rate and flow channel height for each of the two devices: fQCM= 2.64*fSPR(hQCM/hSPR)^2; where fQCM and fSPR are the flow rates in the SPR and QCM flow channels respectively, and hQCM/hSPR is the ratio of the heights of the two channels. We demonstrate the success of our calibration procedure through the combined use of commercially available SPR and QCM flow channel devices on both a biomolecular interaction system of surf ace immobilized biotin and streptavidin and a targeted drug delivery model system of biotinylated liposomes interacting with a streptavidin functionalized surface.

MP-SPR keywords: biotin, hydration, liposome, mass transport, MP-SPR, QCM, shear stress, streptavidin, validation