Bacterial surface, biofilm and virulence properties of Listeriamonocytogenes strains isolated from smoked salmon and fish food contact surfaces

Publication year: 2021
Authors: Sudagidan M. a, Ozalp V.C. b, Öztürk O. c, Zafer Yurt M.N, a, Yavuz O. d, Tasbasi B.B. a, Ucak S. e, Mavili Z.S., Coban A. g, Aydin A. h
Affiliations:

a - KIT-ARGEM R&D Center, Konya Food and Agriculture University, Meram, 42080, Konya, Turkey
b - Medical School, Department of Medical Biology, Atilim University, 06830, Ankara, Turkey
c - Department of Physics, Izmir Institute of Technology, Urla, 35430, Izmir, Turkey
d - Scientific and Technology Application and Research Center, Mehmet Akif Ersoy University, 15030, Burdur, Turkey
e - Medical School, Department of Medical Biology, Istanbul Aydın University, 34295, Istanbul, Turkey
f - Faculty of Health Sciences, Department of Nutrition and Dietetics, Bahcesehir University, 34353, Istanbul, Turkey
g - Department of Gastronomy and Culinary Arts, Istanbul Gedik University, Kartal, 34876, Istanbul, Turkey
h - Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Istanbul University-Cerrahpasa, Avcilar, 34320, Istanbul, Turkey

Published in: Food Bioscience, 2021, Vol. 41, p.101021
doi: 10.1016/j.fbio.2021.101021

Biofilm formation is one of the defense mechanisms of bacteria against disinfectants and antimicrobials. The aim of this study was to determine biofilm-forming L.monocytogenes from fish processing and salmon surfaces. Biofilm formation at 15, 25, 37, and 40 °C from 1 to 6-days period, adhesion to glass, polypropylene and stainless-steel surfaces, bacterial surface charge and hydrophobicity was determined. Adhesion behavior of the strains was evaluated using Surface Plasmon Resonance (SPR) technique. Totally 32 L.monocytogenes strains belonging to serogroups IIa (n:17), IIc(n:14) and IVb(n:1) were detected from 1320 swabs and 16 smoked salmons. Biofilm formation tests revealed that 21 strains form biofilm on microplate by increasing time and temperature. Although all strains strongly formed biofilm on glass surfaces, two strains slightly adhered polypropylene surfaces. High surface roughness of stainless-steel FeCrNi alloy (Ra = 4.15 nm) and CoCrMo alloy (Ra = 10.75 nm) increased biofilm formation of L.monocytogenes on stainless-steel surfaces. Zeta potential results showed that non-biofilm formers were more negatively charged after 6-days and hydrophobicity couldn't give a distinct distribution among biofilm formers and non-formers. SPR analysis method was evaluated to distinguish biofilm formers to adhere SPR gold chip surfaces. PCR results revealed that all strains were positive for hylAiapactAplcAplcBfriflaAinlAinlBinlCinlJ, and lmo1386 genes. Additionally, all strains were susceptible to penicillin, ampicillin, meropenem, erythromycin and trimethoprim-sulfamethoxazole. Biofilm-forming, virulence properties of L.monocytogenes strains isolated from fish processing surfaces and smoked salmons were evaluated and SPR was used to differentiate biofilm formers as a sensitive technique for biofilm studies.


MP-SPR keywords: Au sensor slide, bacterial attachment, biofilm