Adsorption Behavior of Arabinogalactan-Proteins (AGPs) from Acacia senegal Gum at a Solid–Liquid Interface

Publication year: 2021
Authors: Dvantes A. 1, Nigen M. 2, Sanchez C. 2, Renard D. 1
Affiliations:

1 - UR BIA, INRAE Pays de la Loire, 3 impasse Yvette Cauchois, La Géraudière, CS 71627, F-44316 Nantes Cedex 3, France
2 - UR BIA, INRAE Pays de la Loire, 3 impasse Yvette Cauchois, La Géraudière, CS 71627, F-44316 Nantes Cedex 3, France

Published in: Langmuir, 2021, Vol. 37 (35), p. 10547–10559
doi: 10.1021/acs.langmuir.1c01619

Adsorption of five different hyperbranched arabinogalactan-protein (AGP) fractions from Acacia senegal gum was thoroughly studied at the solid–liquid interface using a quartz crystal microbalance with dissipation monitoring (QCM-D), surface plasmon resonance (SPR), and atomic force microscopy (AFM). The impact of the protein/sugar ratio, molecular weight, and aggregation state on the adsorption capacity was investigated by studying AGP fractions with different structural and biochemical features. Adsorption on a solid surface would be primarily driven by the protein moiety of the AGPs through hydrophobic forces and electrostatic interactions. Increasing ionic strength allows the decrease in electrostatic repulsions and, therefore, the formation of high-coverage films with aggregates on the surface. However, the maximum adsorption capacity was not reached by fractions with a higher protein content but by a fraction that contains an average protein quantity and presents a high content of high-molecular-weight AGPs. The results of this thorough study highlighted that the AGP surface adsorption process would depend not only on the protein moiety and high-molecular-weight AGP content but also on other parameters such as the structural accessibility of proteins, the molecular weight distribution, and the AGP flexibility, allowing structural rearrangements on the surface and spreading to form a viscoelastic film.


MP-SPR keywords: layer thickness, protein adsorption, refractive index