Publications: Life science


Glycotriazole-peptides derived from the peptide HSP1: synergistic effect of triazole and saccharide rings on the antifungal activity

Publication year: 2017
Authors: Junior E.F.C. 1, Guimarães C.F.R.C. 2, Franco L.L. 3, Alves R.J. 3, Kato K.C. 4, Martins H.R. 4, de Souza Filho J.D. 2, Bemquerer M.P. 5, Munhoz V.H.O. 6, Resende J.M. 2, Verly R.M. 7
Published in: Amino Acids, 2017, Vol. 49(8), p. 1389-1400
This work proposes a strategy that uses solid-phase peptide synthesis associated with copper(I)-catalyzed azide alkyne cycloaddition reaction to promote the glycosylation of an antimicrobial peptide (HSP1) [...]

Surface plasmon resonance and molecular docking studies of bovine serum albumin interaction with neomycin: kinetic and thermodynamic analysis

Publication year: 2017
Authors: Sharifi M. 1, Dolatabadi J.E.N. 2, Fathi F. 2,3, Zakariazadeh M. 4, Barzegar A. 4, Rashidi M. 2, Tajalli H. 1, Rashidi M.-R. 2
Published in: BioImpacts, 2017, Vol. 7(2), p. 91–97
Introduction: The interactions between biomacromolecules such as serum albumin (SA) and various drugs have attracted increasing research attention in recent years. [...]

Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance

Publication year: 2017
Authors: Mauricio E. Di Ianni 1, Germán A. Islan 2, Cecilia Y. Chain 3, Guillermo R. Castro 2, Alan Talevi 1, María E. Vela 3
Published in: Journal of Nanomaterials, Vol. 2017, 11 pages
The applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development [...]

Binding Kinetics and Lateral Mobility of HSV-1 on End-Grafted Sulfated Glycosaminoglycans

Publication year: 2017
Authors: Peerboom N. 1, Block S. 2, Altgärde N. 1, Wahlsten O. 1, Möller S. 3, Schnabelrauch M. 3, Trybala E. 4, Bergström T. 4, Bally M. 5
Published in: Biophysics Journal, 2017, Vol. 113, Issue 6, p. 1223-1234
Many viruses, including herpes simplex (HSV), are recruited to their host cells via interaction between their envelope glycoproteins and cell-surface glycosaminoglycans (GAGs). This initial attachment [...]

Differential Effect of Bilayer Thickness on Sticholysin Activity

Publication year: 2017
Authors: Palacios-Ortega J. 1, 2, García-Linares S. 1, 2, Rivera-de-Torre E. 1, Gavilanes J.G. 1, Martínez-del-Pozo A. 1, Slotte J.P. 2
Published in: Langmuir, 2017, Vol. 33 (41), p. 11018–11027
In this study, we examined the influence of bilayer thickness on the activity of the actinoporin toxins sticholysin I and II (StnI and StnII) at 25 °C. Bilayer thickness was varied using dimonounsaturated phosphatidylcholine (PC) analogues [...]

Oligomerization Alters Binding Affinity Between Amyloid Beta and a Modulator of Peptide Aggregation

Publication year: 2017
Authors: Hilt S. 1, Rojalin T. 2, 3, Viitala T. 3, Koivuniemi A.3, Bunker A. 3, Wachsmann-Hogiu S. 2, 4, Kálai T. 5, Hideg K. 5, Yliperttula M. 3, 6, Voss J. C. 1
Published in: The Journal of Physical Chemistry C, 2017, Vol. 121 (43), p. 23974–23987
The soluble oligomeric form of the amyloid beta (Aβ) peptide is the major causative agent in the molecular pathogenesis of Alzheimer’s disease (AD). We have previously developed a pyrroline-nitroxyl fluorene compound (SLF) that blocks [...]

Taste masking effect of chlorogenic acid (CGA) on bitter drugs evaluated by taste sensor and surface plasmon resonance on the basis of CGA-Drug interactions

Publication year: 2017
Authors: Shiraishi S.1, Haraguchi T., Nakamura S., Li D., Kojima H., Yoshida M., Uchida T.
Published in: Chemical and Pharmaceutical Bulletin, Vol. 65 (2017), No. 2, p. 127-13
The purpose of this study was to evaluate the taste-masking effects of chlorogenic acid (CGA) on bitter drugs using taste sensor measurements and surface plasmon resonance (SPR) analysis of CGA-drug interactions. [...]

Controlled Immobilization Strategies to Probe Short Hyaluronan-Protein Interactions

Publication year: 2016
Authors: Burcu Baykal Minsky 1,2, Christiane H. Antoni 1,2, Heike Boehm 1,2,3
Published in: Nature Scientific Reports, 2016, Vol. 6, article n. 21608
Well-controlled grafting of small hyaluronan oligosaccharides (sHA) enables novel approaches to investigate biological processes such as angiogenesis, immune reactions and cancer metastasis. [...]

Raman Spectrocopy and surface plasmon resonance as photonic tools for biopharmaceutical applications

Publication year: 2016
Authors: Rojalin Tatu
Published in: Doctoral dissertation (article-based), University of Helsinki
Biophotonics is an emerging area of scientific research that uses light of photons to probe biological specimens, such as tissues, cells and molecules. The field of biophotonics is broad and considerably multidisciplinary. [...]

Phenylboronic-Acid-Based Polymeric Micelles for Mucoadhesive Anterior Segment Ocular Drug Delivery

Publication year: 2016
Authors: Prosperi-Porta G. 1, Kedzior S. 2, Muirhead B. 1, Sheardown H. 1,2
Published in: Biomacromolecules, 2016, Vol. 17(4), p. 1449-57
Topical drug delivery to the front of the eye is extremely inefficient due to effective natural protection mechanisms such as precorneal tear turnover and the relative impermeability of the cornea and sclera tissues. This causes low ocular drug bioavailability, requiring large frequent doses that result in high systemic exposure and side effects.[...]

Toxin-induced pore formation is hindered by intermolecular hydrogen bonding in sphingomyelin bilayers

Publication year: 2016
Authors: Sara García-Linares a, b, Juan Palacios-Ortega a,b, Tomokazu Yasuda b,c, Mia Åstrand b, José G. Gavilanes a, Álvaro Martínez-del-Pozo a, J. Peter Slotte b
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 6, p. 1189–1195
Sticholysin I and II (StnI and StnII) are pore-forming toxins that use sphingomyelin (SM) for membrane binding. We examined how hydrogen bonding among membrane SMs affected the StnI- and StnII-induced pore formation process, resulting in bilayer permeabilization. [...]

Regulation of Sticholysin II-Induced Pore Formation by Lipid Bilayer Composition, Phase State, and Interfacial Properties

Publication year: 2016
Authors: Palacios-Ortega J. 1,2, García-Linares S. 1,2, Åstrand M. 2, Al Sazzad M.A. 2, Gavilanes J.G. 1, Martínez-Del-Pozo Á. 1, Slotte J.P. 2
Published in: Langmuir, 2016, 32(14), p. 3476-84
Sticholysin II (StnII) is a pore-forming toxin that uses sphingomyelin (SM) as the recognition molecule in targeting membranes. After StnII monomers bind to SM, several toxin monomers act in concert to oligomerize into a functional pore. [...]

On-line coupling of surface plasmon resonance optical sensing to size-exclusion chromatography for affinity assessment of antibody samples

Publication year: 2016
Authors: Dina Lakayan a, b, Rob Haselberg a, Wilfried M.A. Niessen a, c, Govert W. Somsen a, Jeroen Kool a
Published in: Journal of Chromatography A, Vol. 1452, p. 81–88
Surface plasmon resonance (SPR) is an optical technique that measures biomolecular interactions. Stand-alone SPR cannot distinguish different binding components present in one sample. Moreover, sample matrix components may show non-specific binding to the sensor surface [...]

Immunological boosting and personalization of oncolytic virotherapies for cancer treatment

Publication year: 2016
Authors: Mari Hirvinen
Published in: Doctoral Dissertation
Cancer is the leading cause of death worldwide creating a need for novel cancer treatments that are more efficient but also safer and more specific. Oncolytic viruses (OVs) have shown a solid safety profile in clinical trials. OVs are nowadays considered immunotherapies because of to their ability to stimulate the host immune system to fight against cancer. [...]

Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation

Publication year: 2016
Authors: Otto K. Kari 1, Tatu Rojalin 2, Stefano Salmaso 3, Michela Barattin 4, Hanna Jarva 5, Seppo Meri 6, Marjo Yliperttula 7, Tapani Viitala 8, Arto Urtti 9
Published in: Drug Delivery and Translational Research, p. 1-13
When nanocarriers are administered into the blood circulation, a complex biomolecular layer known as the “protein corona” associates with their surface. Although the drivers of corona formation are not known, it is widely accepted that this layer mediates biological interactions of the nanocarrier with its surroundings. [...]

Feasibility Study of the Permeability and Uptake of Mesoporous Silica Nanoparticles across the Blood-Brain Barrier

Publication year: 2016
Authors: Habib Baghirov 1,2,3,4, Didem Karaman 2,5, Tapani Viitala 6, Alain Duchanoy 2, Yan-Ru Lou 6, Veronika Mamaeva 1, Evgeny Pryazhnikov 7, Leonard Khiroug 7, Catharina de Lange Davies 4, Cecilia Sahlgren 1,3,8, Jessica M. Rosenholm 2,5
Published in: PLoS ONE 11(8): e0160705
Drug delivery into the brain is impeded by the blood-brain-barrier (BBB) that filters out the vast majority of drugs after systemic administration. In this work, we assessed the transport, uptake and cytotoxicity of promising drug nanocarriers, mesoporous silica nanoparticles (MSNs), [...]

Surfaces Mimicking Glycosaminoglycans Trigger Different Response of Stem Cells via Distinct Fibronectin Adsorption and Reorganization

Publication year: 2016
Authors: Araújo A.R. 1,2, Soares da Costa D. 1,2, Amorim S. 1.2, Reis R.L. 1,2, Pires R.A. 1,2 Pashkuleva I. 1,2
Published in: ACS Applied Materials & Interfaces, 2016, Vol. 8 (42), p. 28428–28436
We report on the utility of a platform created by self-assembled monolayers to investigate the influence of the degree of sulfation of glycosaminoglycans (GAGs) on their interactions with fibronectin (Fn) and the impact [...]

Optimization of lipodisk properties by modification of the extent and density of the PEG corona

Publication year: 2016
Authors: Zetterberg M.M. 1, Ahlgren S. 1, Agmo Hernández V. 1, Parveen N. 2, Edwards K. 1
Published in: Journal of Colloid and Interface Science, Vol. 484 (2016), p. 86–96
Lipodisks are nanosized flat, circular, phospholipid bilayers that are edge-stabilized by polyethylene glycol-conjugated lipids (PEG-lipids). Over the last decade, lipodisks stabilized [...]

Real-Time Label-Free Monitoring of Nanoparticle Cell Uptake

Publication year: 2016
Authors: Suutari T. 1, Silen T. 1, S.en Karaman D. 2,3, Saari H. 1, Desai D. 2, Kerkelä E. 4, Laitinen S. 4, Hanzlikova M. 1, Rosenholm J.M. 2, Yliperttula M. 1,5, Viitala T. 6
Published in: Small
The surface plasmon resonance technique in combination with whole cell sensing is used for the first time for real-time label-free monitoring of nanoparticle cell uptake. The uptake kinetics of several types of nanoparticles [...]

Differential Effect of Membrane Composition on the Pore-Forming Ability of Four Different Sea Anemone Actinoporins

Publication year: 2016
Authors: García-Linares S. 1, 2, Rivera-de-Torre E. 1, Morante K. 3, Tsumoto K. 3, Caaveiro J.M.M. 3, Gavilanes J.G. 1, Slotte J.P. 2, Martínez-del-Pozo A. 1
Published in: Biochemistry, 2016, Vol. 55 (48), p. 6630–6641
Sea anemone actinoporins constitute a protein family of multigene pore-forming toxins (PFT). Equinatoxin II (EqtII), fragaceatoxin C (FraC), and sticholysins I and II (StnI and StnII, respectively), produced by three different sea anemone species, are the only actinoporins [...]