Publications: Life science

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Membrane interactions of the anuran antimicrobial peptide HSP1-NH2: Different aspects of the association to anionic and zwitterionic biomimetic systems

Publication year: 2021
Authors: I.P.Gomes a, T.L.Santos a, A.N.de Souza a., L.O.Nunes a, G.A.Cardoso a,b, C.O.Matos c, L.M.F.Costa a, L.M.Lião c, J.M.Resende b, R.M.Verly a
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, 2021, Vol. 1863 (1), p. 183449
Studies have suggested that antimicrobial peptides act by different mechanisms, such as micellisation, self-assembly of nanostructures and pore formation on the membrane surface. This work presents [...]

Adsorption and Conformation Behavior of Lysozyme on a Gold Surface Determined by QCM-D, MP-SPR, and FTIR

Publication year: 2021
Authors: Komorek P. 1, Martin E. 2, Jachimska B. 1
Published in: International Journal of Molecular Sciences, 2021, Vol. 22(3), p.1322
The physicochemical properties of protein layers at the solid–liquid interface are essential in many biological processes. This study aimed to link the structural analysis of adsorbed lysozyme [...]

Bacterial surface, biofilm and virulence properties of Listeriamonocytogenes strains isolated from smoked salmon and fish food contact surfaces

Publication year: 2021
Authors: Sudagidan M. a, Ozalp V.C. b, Öztürk O. c, Zafer Yurt M.N, a, Yavuz O. d, Tasbasi B.B. a, Ucak S. e, Mavili Z.S., Coban A. g, Aydin A. h
Published in: Food Bioscience, 2021, Vol. 41, p.101021
Biofilm formation is one of the defense mechanisms of bacteria against disinfectants and antimicrobials. The aim of this study was to determine biofilm-forming L.monocytogenes from fish processing [...]

Design and synthesis of quinoline-pyrimidine inspired hybrids as potential plasmodial inhibitors

Publication year: 2021
Authors: Kayamba F. a. Malimabe T. b,c, Ademola I.K. d, Pooe O.J. e, Kushwaha N.D. a, Mahlalela M. a, van Zyl R.L. b,c, Gordon M. d, Mudau P.T. f, Zininga T. f,g, Shonhai A. g, Nyamori V.O. h, Karpoormath R. a
Published in: European Journal of Medicinal Chemistry, 2021, Vol. 217, p. 113330
Presently, artemisinin-based combination therapy (ACT) is the first-line therapy of Plasmodium falciparum malaria. With the emergence of malaria parasites that are resistant to ACT, alternative [...]

A designer FG-Nup that reconstitutes the selective transport barrier of the nuclear pore complex

Publication year: 2021
Authors: Fragasso A. 1, de Vries H.W. 2, Andersson J. 3, van der Sluis E.O. 1, van der Giessen E. 2, Dahlin A. 3, Onck P.R. 2, Dekker C. 1
Published in: Nature Communications, 2021, Vol. 12, 2010 (2021)
Nuclear Pore Complexes (NPCs) regulate bidirectional transport between the nucleus and the cytoplasm. Intrinsically disordered FG-Nups line the NPC lumen and form a selective barrier, where transport of [...]

Kinetic and thermodynamic insights into the interaction of Aβ1–42 with astaxanthin and aggregation behavior of Aβ1–42: Surface plasmon resonance, microscopic, and molecular docking studies

Publication year: 2021
Authors: Moharram Dehghani a, Razieh Jalal a,b, Mohammad-Reza Rashidi c
Published in: Biophysical Chemistry, 2021, Vol.275, p. 106612
Amyloid-β 1–42 (Aβ1–42) aggregation is considered as an important process in the pathology of Alzheimer's disease (AD). Astaxanthin (ATX), a xanthophyll carotenoid, has a broad range [...]

Supporting data on characterisation of linker switch mutants of Plasmodium falciparum heat shock protein 110 and canonical Hsp70

Publication year: 2021
Authors: Graham Chakafana a,b, Pertunia T. Mudau a, Tawanda Zininga a,c, Addmore Shonhai a
Published in: Data in Brief, 2021, Vol. 37, p. 107177
Here, we present data on characterisation of the linker of Plasmodium falciparum Hsp110 (PfHsp70-z) relative to the linker of canonical Hsp70s in support of a co-published article [1]. The linker of PfHsp70-z was [...]

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Publication year: 2021
Authors: Wenqian Lin 1, Jinlai Yang 2, Yayue Zheng 1, Caoxing Huang 1, Qiang Yong 1
Published in: Biotechnology for Biofuels, 2021, Vol. 14, Article number: 143 (2021)
During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. [...]

SPRD: a surface plasmon resonance database of common factors for better experimental planning

Publication year: 2021
Authors: P.B. Tiwari 1, C. Bencheqroun 2, M. Lemus 1, T. Shaw 1, M. Kouassi-Brou 1,3, A. Alaoui 2, A. Üren 1
Published in: BMC Molecular and Cell Biology, Vol. 22, 17 (2021)
Surface plasmon resonance is a label-free biophysical technique that is widely used in investigating biomolecular interactions, including protein-protein, protein-DNA, and protein-small molecule binding. [...]

Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

Publication year: 2021
Authors: Xue Zhang a, Tapani Viitala b,1, Riina Harjumäki a,b, Alma Kartal-Hodzic b, Juan José Valle-Delgado a, Monika Österberg a
Published in: Journal of Colloid and Interface Science, Vol. 584 (2021), p. 310-319
The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. [...]

Terminal deoxynucleotidyl transferase-mediated formation of protein binding polynucleotides

Publication year: 2021
Authors: J. Ashley 1,2, A.-L. Schaap-Johansen 1, M. Mohammadniaei 1, M. Naseri 1, P. Marcatili 1, M. Prado 2, Y. Sun 1
Published in: Nucleic Acids Research, 2021, Vol. 49, Issue 2, p. 1065–1074
Terminal deoxynucleotidyl transferase (TdT) enzyme plays an integral part in the V(D)J recombination, allowing for the huge diversity n expression of immunoglobulins and T-cell receptors [...]

Characterisation of a unique linker segment of the Plasmodium falciparum cytosol localised Hsp110 chaperone

Publication year: 2021
Authors: G. Chakafana a,b, P.T.Mudau a, T.Zininga a,c, A.Shonhai a
Published in: International Journal of Biological Macromolecules, 2021, Vol.180, p. 272-285
Plasmodium falciparum expresses two essential cytosol localised chaperones; PfHsp70-1 and PfHsp70-z. PfHsp70-z (Hsp110 homologue) is thought to facilitate nucleotide exchange function of PfHsp70-1. PfHsp70-1 is a refoldase, [...]

Mutation of GGMP Repeat Segments of Plasmodium falciparum Hsp70-1 Compromises Chaperone Function and Hop Co-Chaperone Binding

Publication year: 2021
Authors: S. Makumire 1,2 , T. Harmfree Dongola 1, G. Chakafana 1,3, L. Tshikonwane 1, C. Tshikani Chauke 1, T. Maharaj 4, T. Zininga 1,4, A. Shonhai 1
Published in: International Journal of Molecular Sciences, 2021, Vol. 22(4), p. 2226
Parasitic organisms especially those of the Apicomplexan phylum, harbour a cytosol localised canonical Hsp70 chaperone. One of the defining features of this protein is the presence of GGMP repeat residues [...]

Delineating the Molecular Basis of the Calmodulin–bMunc13-2 Interaction by Cross-Linking/Mass Spectrometry—Evidence for a Novel CaM Binding Motif in bMunc13-2

Publication year: 2020
Authors: Piotrowski C. 1, Moretti R. 2, Ihling C. 1,Haedicke A. 3†, Liepold T. 4, Lipstein N. 5, Meiler J. 2, Jahn O. 4,*, Sinz A. 1,*
Published in: Cells, 2020, Vol.9 (1) , p.136
Exploring the interactions between the Ca2+ binding protein calmodulin (CaM) and its target proteins remains a challenging task. Members of the Munc13 protein family play an essential role in short-term synaptic plasticity, modulated via the interaction with CaM at the presynaptic compartment. [...]

Single-vesicle imaging reveals lipid-selective and stepwise membrane disruption by monomeric α-synuclein

Publication year: 2020
Authors: Hannestad J. a, Rocha S. b, Agnarsson B. a, Zhdanov V. ac, Wittung-Stafshede P. *b, Höök F. *a
Published in: Proceedings of National Academy of Sciences PNAS, 2020, Vol. 117, 25, p.14178-14186
The interaction of the neuronal protein α-synuclein with lipid membranes appears crucial in the context of Parkinson’s disease, but the underlying mechanistic details, including the roles of different lipids in pathogenic protein aggregation and membrane disruption, remain elusive. Here, we used single-vesicle resolution fluorescence and label-free scattering microscopy to investigate the interaction kinetics of monomeric α-synuclein with surface-tethered vesicles composed of different negatively charged lipids.[...]

Label-free characterization and real-time monitoring of cell uptake of extracellular vesicles

Publication year: 2020
Authors: Koponen A.ab, Kerkelä E. a, Rojalin T. b, Lázaro-Ibáñez E. b, Suutari T. b, Saari H.b, Siljander P.c, Yliperttula M. b, Laitinen S. a, Viitala T. b
Published in: Biosensors and Bioelectronics, 2020, Vol. 168, p. 112510
Extracellular vesicles (EVs) have the ability to function as molecular vehicles and could therefore be harnessed to deliver drugs to target cells in diseases such as cancer. The composition of EVs determines their function as well as their interactions with cells, which consequently affects the cell uptake efficacy of EVs.[...]

Heparin/Collagen Surface Coatings Modulate the Growth, Secretome and Morphology of Human Mesenchymal Stromal Cell Response to Interferon‐Gamma

Publication year: 2020
Authors: Cifuentes S. 1, Priyadarshani P. 23,Castilla‐Casadiego D. 4, Mortensen L. 23,Almodóvar J. 4, Domenech M. 15
Published in: Journal of Biomedical Materials Research, 2020
The therapeutic potential of human mesenchymal stromal cells (h‐MSC) is dependent on the viability and secretory capacity of cells both modulated by the culture environment. Our previous studies introduced heparin and collagen I (HEP/COL) alternating stacked layers as a potential substrate to enhance the secretion of immunosuppressive factors of h‐MSCs. [...]

Partitioning of Catechol Derivatives in Lipid Membranes: Implications for Substrate Specificity to Catechol-O-methyltransferase

Publication year: 2020
Authors: Parkkila P., Viitala T.
Published in: ACS Chemical Neuroscience, 2020, Vol.11, 6, p. 969–978
We have utilized multiparametric surface plasmon resonance and impendance-based quartz crystal microbalance instruments to study the distribution coefficients of catechol derivatives in cell model membranes. Our findings verify that the octanol–water partitioning coefficient is a poor descriptor of the total lipid affinity for small molecules which show limited lipophilicity in the octanol–water system.[...]

Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy

Publication year: 2020
Authors: Vilaró P. 1,†, Sampl C. 2,†,Teichert G. 2, Schlemmer W. 2, Hobisch M. 2, Weissl M. 2, Panizzolo L. 3, Ferreira F. 1, Spirk S. 2,*
Published in: Polymers, 2020, Vol. 12(12), p.3040
Interactions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively [...]

Diffusion and Protein Corona Formation of Lipid-Based Nanoparticles in the Vitreous Humor: Profiling and Pharmacokinetic Considerations

Publication year: 2020
Authors: Tavakoli S 1., Kari O.K. 1, Turunen T. 1, Lajunen T. 1, Schmitt M. 1, Lehtinen J. 1, Tasaka F. 2, Parkkila P. 1, Ndika J. 3, Viitala T. 1, Alenius H. 3,5, Urtti, A. 1,6,7 Subriz A. *7
Published in: Molecular Pharmaceutics, 2020
The vitreous humor is the first barrier encountered by intravitreally injected nanoparticles. Lipid-based nanoparticles in the vitreous are studied by evaluating their diffusion with single-particle tracking technology and by characterizing their protein coronae with surface plasmon resonance and high-resolution proteomics. Single-particle tracking results indicate that the vitreal mobility of the formulations is dependent on their charge.[...]