Publications: Life science

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SPRD: a surface plasmon resonance database of common factors for better experimental planning

Publication year: 2021
Authors: P.B. Tiwari 1, C. Bencheqroun 2, M. Lemus 1, T. Shaw 1, M. Kouassi-Brou 1,3, A. Alaoui 2, A. Üren 1
Published in: BMC Molecular and Cell Biology, Vol. 22, 17 (2021)
Surface plasmon resonance is a label-free biophysical technique that is widely used in investigating biomolecular interactions, including protein-protein, protein-DNA, and protein-small molecule binding. [...]

Adhesion and Structural Changes of PEGylated Lipid Nanocarriers on Silica Surfaces

Publication year: 2021
Authors: Grad P. 1, Edwards K. 1, Hernández V.A. 2
Published in: Physchem, 2021, Vol. 1(2), p. 133-151
PEGylated lipid nanoparticles have a continuously expanding range of applications, particularly within pharmaceutical areas. Hereby, it is shown with the help of [...]

Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

Publication year: 2021
Authors: Xue Zhang a, Tapani Viitala b,1, Riina Harjumäki a,b, Alma Kartal-Hodzic b, Juan José Valle-Delgado a, Monika Österberg a
Published in: Journal of Colloid and Interface Science, Vol. 584 (2021), p. 310-319
The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. [...]

Membrane interactions of Ocellatins. Where do antimicrobial gaps stem from?

Publication year: 2021
Authors: J.Muñoz‑López 1,2, J.C.L.Oliveira 1, D.A.G.R. Michel 3, C.S. Ferreira 3, F.Gomes Neto 4, E.S.Salnikov 2, R.M.Verly 3, B.Bechinger 2,5, J.M.Resende 1
Published in: Amino Acids, Vol. 53, p. 1241–1256 (2021)
The antimicrobial peptides Ocellatin-LB1, -LB2 and -F1, isolated from frogs, are identical from residue 1 to 22, which correspond to the -LB1 sequence, whereas -LB2 carries an extra N and -F1 additional NKL residues [...]

Terminal deoxynucleotidyl transferase-mediated formation of protein binding polynucleotides

Publication year: 2021
Authors: J. Ashley 1,2, A.-L. Schaap-Johansen 1, M. Mohammadniaei 1, M. Naseri 1, P. Marcatili 1, M. Prado 2, Y. Sun 1
Published in: Nucleic Acids Research, 2021, Vol. 49, Issue 2, p. 1065–1074
Terminal deoxynucleotidyl transferase (TdT) enzyme plays an integral part in the V(D)J recombination, allowing for the huge diversity n expression of immunoglobulins and T-cell receptors [...]

Adsorption Behavior of Arabinogalactan-Proteins (AGPs) from Acacia senegal Gum at a Solid–Liquid Interface

Publication year: 2021
Authors: Dvantes A. 1, Nigen M. 2, Sanchez C. 2, Renard D. 1
Published in: Langmuir, 2021, Vol. 37 (35), p. 10547–10559
Adsorption of five different hyperbranched arabinogalactan-protein (AGP) fractions from Acacia senegal gum was thoroughly studied at the solid–liquid interface using a quartz crystal microbalance with dissipation monitoring [...]

Characterisation of a unique linker segment of the Plasmodium falciparum cytosol localised Hsp110 chaperone

Publication year: 2021
Authors: G. Chakafana a,b, P.T.Mudau a, T.Zininga a,c, A.Shonhai a
Published in: International Journal of Biological Macromolecules, 2021, Vol.180, p. 272-285
Plasmodium falciparum expresses two essential cytosol localised chaperones; PfHsp70-1 and PfHsp70-z. PfHsp70-z (Hsp110 homologue) is thought to facilitate nucleotide exchange function of PfHsp70-1. PfHsp70-1 is a refoldase, [...]

Hyaluronic Acid Oligomer Immobilization as an Angiogenic Trigger for the Neovascularization of TE Constructs

Publication year: 2021
Authors: A.L. Silva 1,2, P.S. Babo 1,2, M.T. Rodrigues 1,2, A.I. Gonçalves 1,2, R. Novoa-Carballal 1,2, R.A. Pires 1,2, J. Rouwkema 3, R.L. Reis 1,2, M.E.Gomes 1,2
Published in: ACS Applied Bio Materials, 2021, Vol. 4 (8), p. 6023–6035
Tissue engineered (TE) substitutes of clinically relevant sizes need an adequate vascular system to ensure function and proper tissue integration after implantation. However, the predictable vascularization [...]

Mutation of GGMP Repeat Segments of Plasmodium falciparum Hsp70-1 Compromises Chaperone Function and Hop Co-Chaperone Binding

Publication year: 2021
Authors: S. Makumire 1,2 , T. Harmfree Dongola 1, G. Chakafana 1,3, L. Tshikonwane 1, C. Tshikani Chauke 1, T. Maharaj 4, T. Zininga 1,4, A. Shonhai 1
Published in: International Journal of Molecular Sciences, 2021, Vol. 22(4), p. 2226
Parasitic organisms especially those of the Apicomplexan phylum, harbour a cytosol localised canonical Hsp70 chaperone. One of the defining features of this protein is the presence of GGMP repeat residues [...]

Insight into dual fluorescence effects induced by molecular aggregation occurring in membrane model systems containing 1,3,4-thiadiazole derivatives

Publication year: 2021
Authors: David M. 1, Budziak-Wieczorek I. 2, Karcz D. 3, Florescu M. 1, Matwijczuk A. 4
Published in: European Biophysics Journal (2021)
This work reports on biophysical insights into the excited state intramolecular proton transfer (ESIPT) processes taking place in three 1,3,4-thiadiazole derivatives that served as model compounds, [...]

Membrane interactions of the anuran antimicrobial peptide HSP1-NH2: Different aspects of the association to anionic and zwitterionic biomimetic systems

Publication year: 2021
Authors: I.P.Gomes a, T.L.Santos a, A.N.de Souza a., L.O.Nunes a, G.A.Cardoso a,b, C.O.Matos c, L.M.F.Costa a, L.M.Lião c, J.M.Resende b, R.M.Verly a
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, 2021, Vol. 1863 (1), p. 183449
Studies have suggested that antimicrobial peptides act by different mechanisms, such as micellisation, self-assembly of nanostructures and pore formation on the membrane surface. This work presents [...]

Lipid Cubic Systems for Sustained and Controlled Delivery of Antihistamine Drugs

Publication year: 2021
Authors: Dully M. 1, Ceresnakova M. 1, Murray D. 2, Souliname T. 1, Hudson S.P. 1
Published in: Molecular Pharmaceutic, 2021, Vol. 18(10), p. 3777–3794
Antihistamines are capable of blocking mediator responses in allergic reactions including allergic rhinitis and dermatological reactions. By incorporating various H1 receptor antagonists into [...]

Adsorption and Conformation Behavior of Lysozyme on a Gold Surface Determined by QCM-D, MP-SPR, and FTIR

Publication year: 2021
Authors: Komorek P. 1, Martin E. 2, Jachimska B. 1
Published in: International Journal of Molecular Sciences, 2021, Vol. 22(3), p.1322
The physicochemical properties of protein layers at the solid–liquid interface are essential in many biological processes. This study aimed to link the structural analysis of adsorbed lysozyme [...]

Bacterial surface, biofilm and virulence properties of Listeriamonocytogenes strains isolated from smoked salmon and fish food contact surfaces

Publication year: 2021
Authors: Sudagidan M. a, Ozalp V.C. b, Öztürk O. c, Zafer Yurt M.N, a, Yavuz O. d, Tasbasi B.B. a, Ucak S. e, Mavili Z.S., Coban A. g, Aydin A. h
Published in: Food Bioscience, 2021, Vol. 41, p.101021
Biofilm formation is one of the defense mechanisms of bacteria against disinfectants and antimicrobials. The aim of this study was to determine biofilm-forming L.monocytogenes from fish processing [...]

Design and synthesis of quinoline-pyrimidine inspired hybrids as potential plasmodial inhibitors

Publication year: 2021
Authors: Kayamba F. a. Malimabe T. b,c, Ademola I.K. d, Pooe O.J. e, Kushwaha N.D. a, Mahlalela M. a, van Zyl R.L. b,c, Gordon M. d, Mudau P.T. f, Zininga T. f,g, Shonhai A. g, Nyamori V.O. h, Karpoormath R. a
Published in: European Journal of Medicinal Chemistry, 2021, Vol. 217, p. 113330
Presently, artemisinin-based combination therapy (ACT) is the first-line therapy of Plasmodium falciparum malaria. With the emergence of malaria parasites that are resistant to ACT, alternative [...]

A designer FG-Nup that reconstitutes the selective transport barrier of the nuclear pore complex

Publication year: 2021
Authors: Fragasso A. 1, de Vries H.W. 2, Andersson J. 3, van der Sluis E.O. 1, van der Giessen E. 2, Dahlin A. 3, Onck P.R. 2, Dekker C. 1
Published in: Nature Communications, 2021, Vol. 12, 2010 (2021)
Nuclear Pore Complexes (NPCs) regulate bidirectional transport between the nucleus and the cytoplasm. Intrinsically disordered FG-Nups line the NPC lumen and form a selective barrier, where transport of [...]

Kinetic and thermodynamic insights into the interaction of Aβ1–42 with astaxanthin and aggregation behavior of Aβ1–42: Surface plasmon resonance, microscopic, and molecular docking studies

Publication year: 2021
Authors: Moharram Dehghani a, Razieh Jalal a,b, Mohammad-Reza Rashidi c
Published in: Biophysical Chemistry, 2021, Vol.275, p. 106612
Amyloid-β 1–42 (Aβ1–42) aggregation is considered as an important process in the pathology of Alzheimer's disease (AD). Astaxanthin (ATX), a xanthophyll carotenoid, has a broad range [...]

Supporting data on characterisation of linker switch mutants of Plasmodium falciparum heat shock protein 110 and canonical Hsp70

Publication year: 2021
Authors: Graham Chakafana a,b, Pertunia T. Mudau a, Tawanda Zininga a,c, Addmore Shonhai a
Published in: Data in Brief, 2021, Vol. 37, p. 107177
Here, we present data on characterisation of the linker of Plasmodium falciparum Hsp110 (PfHsp70-z) relative to the linker of canonical Hsp70s in support of a co-published article [1]. The linker of PfHsp70-z was [...]

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Publication year: 2021
Authors: Wenqian Lin 1, Jinlai Yang 2, Yayue Zheng 1, Caoxing Huang 1, Qiang Yong 1
Published in: Biotechnology for Biofuels, 2021, Vol. 14, Article number: 143 (2021)
During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. [...]

Surface Plasmon Resonance Monitoring of Mono-Rhamnolipid Interaction with Phospholipid-Based Liposomes

Publication year: 2021
Authors: Belkilani M. 1,2.3, Shokouhi M. 4, Farre C. 1, Chevalier Y. 1, Minot S. 1, Bessueille F. 1, Abdelghani A. 3, Jaffrezic-Renault N. 1, Chaix C. 1
Published in: Langmuir, 2021, Vol. 37 (26), p. 7975–7985
The interactions of mono-rhamnolipids (mono-RLs) with model membranes were investigated through a biomimetic approach using phospholipid-based liposomes immobilized on a gold substrate [...]