Publications: Life science


SPRD: a surface plasmon resonance database of common factors for better experimental planning

Publication year: 2021
Authors: P.B. Tiwari 1, C. Bencheqroun 2, M. Lemus 1, T. Shaw 1, M. Kouassi-Brou 1,3, A. Alaoui 2, A. Üren 1
Published in: BMC Molecular and Cell Biology, Vol. 22, 17 (2021)
Surface plasmon resonance is a label-free biophysical technique that is widely used in investigating biomolecular interactions, including protein-protein, protein-DNA, and protein-small molecule binding. [...]

Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

Publication year: 2021
Authors: Xue Zhang a, Tapani Viitala b,1, Riina Harjumäki a,b, Alma Kartal-Hodzic b, Juan José Valle-Delgado a, Monika Österberg a
Published in: Journal of Colloid and Interface Science, Vol. 584 (2021), p. 310-319
The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. [...]

Terminal deoxynucleotidyl transferase-mediated formation of protein binding polynucleotides

Publication year: 2021
Authors: J. Ashley 1,2, A.-L. Schaap-Johansen 1, M. Mohammadniaei 1, M. Naseri 1, P. Marcatili 1, M. Prado 2, Y. Sun 1
Published in: Nucleic Acids Research, 2021, Vol. 49, Issue 2, p. 1065–1074
Terminal deoxynucleotidyl transferase (TdT) enzyme plays an integral part in the V(D)J recombination, allowing for the huge diversity n expression of immunoglobulins and T-cell receptors [...]

Characterisation of a unique linker segment of the Plasmodium falciparum cytosol localised Hsp110 chaperone

Publication year: 2021
Authors: G. Chakafana a,b, P.T.Mudau a, T.Zininga a,c, A.Shonhai a
Published in: International Journal of Biological Macromolecules, 2021, Vol.180, p. 272-285
Plasmodium falciparum expresses two essential cytosol localised chaperones; PfHsp70-1 and PfHsp70-z. PfHsp70-z (Hsp110 homologue) is thought to facilitate nucleotide exchange function of PfHsp70-1. PfHsp70-1 is a refoldase, [...]

Mutation of GGMP Repeat Segments of Plasmodium falciparum Hsp70-1 Compromises Chaperone Function and Hop Co-Chaperone Binding

Publication year: 2021
Authors: S. Makumire 1,2 , T. Harmfree Dongola 1, G. Chakafana 1,3, L. Tshikonwane 1, C. Tshikani Chauke 1, T. Maharaj 4, T. Zininga 1,4, A. Shonhai 1
Published in: International Journal of Molecular Sciences, 2021, Vol. 22(4), p. 2226
Parasitic organisms especially those of the Apicomplexan phylum, harbour a cytosol localised canonical Hsp70 chaperone. One of the defining features of this protein is the presence of GGMP repeat residues [...]

Membrane interactions of the anuran antimicrobial peptide HSP1-NH2: Different aspects of the association to anionic and zwitterionic biomimetic systems

Publication year: 2021
Authors: I.P.Gomes a, T.L.Santos a, Souza a., L.O.Nunes a, G.A.Cardoso a,b, C.O.Matos c, L.M.F.Costa a, L.M.Lião c, J.M.Resende b, R.M.Verly a
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, 2021, Vol. 1863 (1), p. 183449
Studies have suggested that antimicrobial peptides act by different mechanisms, such as micellisation, self-assembly of nanostructures and pore formation on the membrane surface. This work presents [...]

Adsorption and Conformation Behavior of Lysozyme on a Gold Surface Determined by QCM-D, MP-SPR, and FTIR

Publication year: 2021
Authors: Komorek P. 1, Martin E. 2, Jachimska B. 1
Published in: International Journal of Molecular Sciences, 2021, Vol. 22(3), p.1322
The physicochemical properties of protein layers at the solid–liquid interface are essential in many biological processes. This study aimed to link the structural analysis of adsorbed lysozyme [...]

Bacterial surface, biofilm and virulence properties of Listeriamonocytogenes strains isolated from smoked salmon and fish food contact surfaces

Publication year: 2021
Authors: Sudagidan M. a, Ozalp V.C. b, Öztürk O. c, Zafer Yurt M.N, a, Yavuz O. d, Tasbasi B.B. a, Ucak S. e, Mavili Z.S., Coban A. g, Aydin A. h
Published in: Food Bioscience, 2021, Vol. 41, p.101021
Biofilm formation is one of the defense mechanisms of bacteria against disinfectants and antimicrobials. The aim of this study was to determine biofilm-forming L.monocytogenes from fish processing [...]

Design and synthesis of quinoline-pyrimidine inspired hybrids as potential plasmodial inhibitors

Publication year: 2021
Authors: Kayamba F. a. Malimabe T. b,c, Ademola I.K. d, Pooe O.J. e, Kushwaha N.D. a, Mahlalela M. a, van Zyl R.L. b,c, Gordon M. d, Mudau P.T. f, Zininga T. f,g, Shonhai A. g, Nyamori V.O. h, Karpoormath R. a
Published in: European Journal of Medicinal Chemistry, 2021, Vol. 217, p. 113330
Presently, artemisinin-based combination therapy (ACT) is the first-line therapy of Plasmodium falciparum malaria. With the emergence of malaria parasites that are resistant to ACT, alternative [...]

Generic High-Capacity Electrochemical Capture and Release of Proteins by Polyelectrolyte Brushes

Publication year: 2020
Authors: Ferrand-Drake del Castillo G., Adali Z., L. N. Hailes R., Xiong K., Dahlin A.*
Published in: Preprint in Chem RXIV, 2020
We present a polyelectrolyte brush electrode with high-capacity for protein immobilization that captures and releases proteins by an electrochemical potential. Central to our concept is the use of polyelectrolyte brushes that switch reversibly by electrochemistry.[...]

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Publication year: 2020
Authors: Dedisch S. 12, Wiens A. 2, Davari M.D. 2, Söder D. 1, Rodriguez‐Emmenegger C. 13, Jakob F. 12, Schwaneberg U.*12
Published in: Biotechnology and Bioengineering, 2020, Vol. 117, Issue1, p. 49-61
Enzyme immobilization is extensively studied to improve enzyme properties in catalysis and analytical applications. Here, we introduce a simple and versatile enzyme immobilization platform based on adhesion‐promoting peptides, namely Matter‐tags. Matter‐tags immobilize enzymes in an oriented way as a dense monolayer.[...]

In situ analysis of liposome hard and soft protein corona structure and composition in a single label-free workflow

Publication year: 2020
Authors: Kari O. *ab, Ndika J. c, Parkkila P. b, Louna A. a, Lajunen T. ǂa, Puustinen A. d, Viitala T. *§b, Alenius H. ce, Urtti A. af
Published in: Nanoscale, 2020, Vol. 12, p. 1728
Methodological constraints have limited our ability to study protein corona formation, slowing nanomedicine development and their successful translation into the clinic. We determined hard and soft corona structural properties along with the corresponding proteomic compositions on liposomes in a label-free workflow: surface plasmon resonance and a custom biosensor for in situ structure determination on liposomes and corona separation, and proteomics using sensitive nanoliquid chromatography tandem mass spectrometry with open-source bioinformatics platforms.[...]

Surfactant Interactions with Protein-Coated Surfaces: Comparison between Colloidal and Macroscopically Flat Surfaces

Publication year: 2020
Authors: Mateos H. 1,*, Valentini A. 2, Lopez F. 3, Palazzo G. 4
Published in: Biomimetics 2020, Vol. 5(3), p. 31
Surface interactions with polymers or proteins are extensively studied in a range of industrial and biomedical applications to control surface modification, cleaning, or biofilm formation. In this study we compare surfactant interactions with protein-coated silica surfaces differing in the degree of curvature (macroscopically flat and colloidal nanometric spheres). The interaction with a flat surface was probed by means of surface plasmon resonance (SPR) while dynamic light scattering (DLS) was used to study the interaction with colloidal SiO2 (radius 15 nm).Surface interactions with polymers or proteins are extensively studied in a range of industrial and biomedical applications to control surface modification, cleaning, or biofilm formation. In this study we compare surfactant interactions with protein-coated silica surfaces differing in the degree of curvature (macroscopically flat and colloidal nanometric spheres). The interaction with a flat surface was probed by means of surface plasmon resonance (SPR) while dynamic light scattering (DLS) was used to study the interaction with colloidal SiO2 (radius 15 nm).[...]

Delineating the Molecular Basis of the Calmodulin–bMunc13-2 Interaction by Cross-Linking/Mass Spectrometry—Evidence for a Novel CaM Binding Motif in bMunc13-2

Publication year: 2020
Authors: Piotrowski C. 1, Moretti R. 2, Ihling C. 1,Haedicke A. 3†, Liepold T. 4, Lipstein N. 5, Meiler J. 2, Jahn O. 4,*, Sinz A. 1,*
Published in: Cells, 2020, Vol.9 (1) , p.136
Exploring the interactions between the Ca2+ binding protein calmodulin (CaM) and its target proteins remains a challenging task. Members of the Munc13 protein family play an essential role in short-term synaptic plasticity, modulated via the interaction with CaM at the presynaptic compartment. [...]

Single-vesicle imaging reveals lipid-selective and stepwise membrane disruption by monomeric α-synuclein

Publication year: 2020
Authors: Hannestad J. a, Rocha S. b, Agnarsson B. a, Zhdanov V. ac, Wittung-Stafshede P. *b, Höök F. *a
Published in: Proceedings of National Academy of Sciences PNAS, 2020, Vol. 117, 25, p.14178-14186
The interaction of the neuronal protein α-synuclein with lipid membranes appears crucial in the context of Parkinson’s disease, but the underlying mechanistic details, including the roles of different lipids in pathogenic protein aggregation and membrane disruption, remain elusive. Here, we used single-vesicle resolution fluorescence and label-free scattering microscopy to investigate the interaction kinetics of monomeric α-synuclein with surface-tethered vesicles composed of different negatively charged lipids.[...]

Label-free characterization and real-time monitoring of cell uptake of extracellular vesicles

Publication year: 2020
Authors: Koponen A.ab, Kerkelä E. a, Rojalin T. b, Lázaro-Ibáñez E. b, Suutari T. b, Saari H.b, Siljander P.c, Yliperttula M. b, Laitinen S. a, Viitala T. b
Published in: Biosensors and Bioelectronics, 2020, Vol. 168, p. 112510
Extracellular vesicles (EVs) have the ability to function as molecular vehicles and could therefore be harnessed to deliver drugs to target cells in diseases such as cancer. The composition of EVs determines their function as well as their interactions with cells, which consequently affects the cell uptake efficacy of EVs.[...]

Heparin/Collagen Surface Coatings Modulate the Growth, Secretome and Morphology of Human Mesenchymal Stromal Cell Response to Interferon‐Gamma

Publication year: 2020
Authors: Cifuentes S. 1, Priyadarshani P. 23,Castilla‐Casadiego D. 4, Mortensen L. 23,Almodóvar J. 4, Domenech M. 15
Published in: Journal of Biomedical Materials Research, 2020
The therapeutic potential of human mesenchymal stromal cells (h‐MSC) is dependent on the viability and secretory capacity of cells both modulated by the culture environment. Our previous studies introduced heparin and collagen I (HEP/COL) alternating stacked layers as a potential substrate to enhance the secretion of immunosuppressive factors of h‐MSCs. [...]

Partitioning of Catechol Derivatives in Lipid Membranes: Implications for Substrate Specificity to Catechol-O-methyltransferase

Publication year: 2020
Authors: Parkkila P., Viitala T.
Published in: ACS Chemical Neuroscience, 2020, Vol.11, 6, p. 969–978
We have utilized multiparametric surface plasmon resonance and impendance-based quartz crystal microbalance instruments to study the distribution coefficients of catechol derivatives in cell model membranes. Our findings verify that the octanol–water partitioning coefficient is a poor descriptor of the total lipid affinity for small molecules which show limited lipophilicity in the octanol–water system.[...]

Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy

Publication year: 2020
Authors: Vilaró P. 1,†, Sampl C. 2,†,Teichert G. 2, Schlemmer W. 2, Hobisch M. 2, Weissl M. 2, Panizzolo L. 3, Ferreira F. 1, Spirk S. 2,*
Published in: Polymers, 2020, Vol. 12(12), p.3040
Interactions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively [...]

Diffusion and Protein Corona Formation of Lipid-Based Nanoparticles in the Vitreous Humor: Profiling and Pharmacokinetic Considerations

Publication year: 2020
Authors: Tavakoli S 1., Kari O.K. 1, Turunen T. 1, Lajunen T. 1, Schmitt M. 1, Lehtinen J. 1, Tasaka F. 2, Parkkila P. 1, Ndika J. 3, Viitala T. 1, Alenius H. 3,5, Urtti, A. 1,6,7 Subriz A. *7
Published in: Molecular Pharmaceutics, 2020
The vitreous humor is the first barrier encountered by intravitreally injected nanoparticles. Lipid-based nanoparticles in the vitreous are studied by evaluating their diffusion with single-particle tracking technology and by characterizing their protein coronae with surface plasmon resonance and high-resolution proteomics. Single-particle tracking results indicate that the vitreal mobility of the formulations is dependent on their charge.[...]