Surface plasmon resonance analysis for detecting non-structural protein 1 of dengue virus in Indonesia

Authors: Widoretno 1 2, Sjahrurachman A. 3, Dewi B.E. 4, Lischer K. 5 6, Pratami D.K. 7, Flamandita D. 5, Sahlan M. 5 6

1 - Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta 10430, Indonesia.
2 - The National Institute of Health Research Development, The Ministry of Health of the Republic of Indonesia, Central Jakarta, Jakarta Capital         Special Region 10560, Indonesia.
3 - Department of Microbiology, Faculty of Medicine, Universitas Indonesia, DKI Jakarta 10430, Indonesia.
4 - Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, DKI Jakarta 10430, Indonesia.
5 - Bioprocess Technology, Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java 16424,                   Indonesia.
6 - Research Center for Biomedical Engineering, Universitas Indonesia, Depok, West Java 16424, Indonesia.
7 - Lab of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Pancasila University, Jakarta 12640, Indonesia.

Published in: Saudi Journal of Biological Sciences, 2020, Vol. 8, p.1931-1937
doi: 10.1016/j.sjbs.2020.06.018

Dengue is an acute febrile disease caused by dengue virus (DENV) that is transmitted by Aedes sp., which causes serious health conditions in many countries. Non-structural protein 1 (NS1) is a co-factor for the RNA replication of this virus, which represents a new strategy for the identification of dengue. Prompt and accurate laboratory diagnosis of this infection is required to assist in patient triage and management, as well as prevent the spread of this infection. In the present study, we tested the potential of surface plasmon resonance (SPR) as a diagnostic tool for dengue infections. NS1 antigen protein was used as an analyte that targets anti-NS1 antibodies, with their interaction resulting in a change in the refractive index. In comparison to currently available gold-standard detection methods [enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR)], SPR showed a similar sensitivity but greater efficiency and simplicity in terms of infection detection. Out of 26 samples collected from patients with dengue in Indonesia, SPR was able to correctly identify all 16 positively infected individuals at a lower concentration and a shorter period of time compared to ELISA and RT-PCR. This study revealed that SPR is a promising tool for DENV detection and potentially other diseases as well.

MP-SPR keywords: antibody-antigen, binding, biosensor, dengue fever