In-situ and real-time probing cellulase biosensor formation and its interaction with lignosulfonate in varied media

Publication year: 2021
Authors: Peipei Wang a,1, Tian Liu a,1, Yena Liu a,1, Jing Tian a, Xinyu Zhang a, Jiaqi Guo a, Yongcan Jin a, Huining Xiao b, Junlong Song a

a - Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources and Joint International Research Lab of Lignocellulosic Functional Materials, Nanjing Forestry University, Nanjing 210037, China
b - Department of Chemical Engineering, University of New Brunswick, Fredericton, NB, E3B 5A3 Canada

Published in: Sensors and Actuators B: Chemical, Vol. 329 (2021), 129114
doi: 10.1016/j.snb.2020.129114

It is a challenge to interpret the signals obtained by Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) accompanied with in the significant changes of the density and/or refractivity of media. The work presented herein focuses on demonstrating a universal in-situ and real-time monitoring methodology for ultrathin film formation on gold chips in varied media. Biomimetic model cellulase/enzyme film was immobilized in-situ on gold surface of sensors, followed by revealing its interaction with lignosulphonate in the media changed from ethanol-based to aqueous ones. Combining the techniques of Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and Multi-parametric Surface Plasmon Resonance (MP-SPR) along with respective software and models enabled us to precisely characterize the thickness, viscous and shear elastic moduli, and the coupled solvent for individual layer formed over the entire process, which are unachievable with conventional methods. The methodology provides real-time monitoring of filming process and offers us an in-situ qualify control approach to the biomimetic model films formed on different substrates including gold chip.

MP-SPR keywords: Au sensor slide, enzyme, layer thickness, ultrathin film