Kinetic and thermodynamic studies of bovine serum albumin interaction with ascorbyl palmitate and ascorbyl stearate food additives using surface plasmon resonance

Publication year: 2018
Authors: Fathi F. a, Mohammadzadeh-Aghdash H. b, c, Sohrabi Y. b, c, Dehghan P. b, Ezzati J. N. D. a

a - Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
b - Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
c - Student research committee, Tabriz University of Medical Sciences, Tabriz, Iran

Published in: Food Chemistry, 2018, Vol. 246, p.228-232
doi: 10.1016/j.foodchem.2017.11.023

Ascorbyl palmitate (AP) and ascorbyl stearate (AS) are examples of food additives, which have extensive use in food industry. In this study, we evaluated the interaction of bovine serum albumin (BSA) with AP and AS using surface plasmon resonance (SPR). In order to immobilize BSA, carboxymethyl dextran hydrogel (CMD) Au chip was used. After activation of carboxylic groups, BSA was immobilized onto the CMD chip through covalent amide binding formation. AP and AS binding to immobilized BSA at different concentrations was assessed. The dose–response sensorgrams of BSA upon increasing concentration of AP and AS have been shown. The low value of equilibrium dissociation constant or affinity unit (KD) showed high affinity of both AP and AS to BSA. The KD value for binding of AP and AS to BSA were 4.09 × 10−5 and 1.89 × 10−5, at 25 °C. Overall, the attained results showed that AP and AS molecules can bind to BSA.

MP-SPR keywords: affinity, biosensor, bovine serum albumin (BSA), food additives, immobilization of BSA using amino coupling chemistry, interaction of ascorbyl palmitate and ascorbyl stearate, kinetics, measurement in different temperatures, thermodynamic study