Functional Plasma Polymerized Surfaces for Biosensing

Publication year: 2020
Authors: Makhneva* E., Barillas L 1., Farka Z. 2, Pastucha M. 2, Skládal P. 2, Weltmann K.D. 1, Fricke K. 1

1 − RG Bio Sensing Surfaces, Leibniz Institute for Plasma Science and Technology (INP), 17489 Greifswald, Germany
2 − RG Nanobiotechnology, Central European Institute of Technology (CEITEC), Masaryk University, 625 00 Brno, Czech Republic

Published in: Applied Material Interfaces, 2020, Vol.12, 14, p.17100–17112
doi: 10.1021/acsami.0c01443

The capabilities of biosensors for fast, economic, and user-friendly analysis of complex samples has led to the exploitation of analytical devices for detection, quantification, and monitoring of specific chemical species for various applications. For a sufficiently high surface reactivity toward the adopted bioreceptors, a thin functional layer is required to enable coupling of the target biomolecules and to provide good stability in the presence of a sample matrix. In this work, the generation of water-stable oxygen-rich plasma polymerized (pp) films deposited by atmospheric-pressure jet plasma for reliable immobilization of biomolecules is presented. Three types of pp films were developed and characterized. All of the obtained pp films were successfully used as a matrix layer in the SPR immunosensors, which provided excellent level of sensitivity, stability, and regenerability. The achieved results show that atmospheric pressure plasma-induced polymerization is a powerful alternative method for the preparation of matrix layers for a wide range of applications in the biological field.

MP-SPR keywords: antibody, antibody-antigen interaction, biosensor, immunosensor, interaction, plasma polymerization