Early-stage detection of VE-cadherin during endothelial differentiation of human mesenchymal stem cells using SPR biosensor

Publication year: 2017
Authors: Fathi F. 1, Rezabakhsh A. 2, Rahbarghazi R. 3, Rashidi M.R. 4
Affiliations:

1 - Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 - Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
3 - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
4 - Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran; Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Published in: Biosensors and Bioelectronics, 2017, Vol. 96, p. 358-366
doi: 10.1016/j.bios.2017.05.018

Surface plasmon resonance (SPR) biosensors are most commonly applied for real-time dynamic analysis and measurement of interactions in bio-molecular studies and cell-surface analysis without the need for labeling processes. Up to present, SPR application in stem cell biology and biomedical sciences was underused. Herein, a very simple and sensitive method was developed to evaluate human mesenchymal stem cells trans-differentiation to endothelial lineage of over a period of 14 days based on VE-cadherin biomarker. The SPR signals increased with the increase of the amount of VE-cadherin expression on the cell surface during cell differentiation process. The method was able to detect ≈27 cells permm2. No significant effect was observed on the cell viability during the cell attachment to the surface of immune-reactive biochips and during the SPR analysis. Using this highly sensitive SPR method, it was possible to sense the early stage of endothelial differentiation on day 3 in label-free form, whereas flow cytometry and fluorescent microscopy methods were found unable to detect the cell differentiation at the same time. Therefore, the proposed method can rapidly and accurately detect cell differentiation in live cells and label-free manner without any need of cell breakage and has great potential for both diagnostic and experimental approaches.


MP-SPR keywords: antibody coupling on self-assembly layer (SAM) using amine coupling chemistry, antibody – cell interactions, biomarker, cell capture by antibody, detecting VE-cadherin expression in cells, detection of stem cells differentiation, early detection of differentiation, label-free detection of differentiation, vascular endothelial-cadherin (VE-cadherin) antibody