Publications: Biosensors

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Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Publication year: 2015
Authors: Miyazaki C.M. 1, Pereira T.P. 1, Tavares Mascagni D.B. 2, de Moraes M.L. 3, Ferreira M. 1
Published in: Materials Science and Engineering: C, 2015, Vol. 58, p. 310–315
In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. [...]

Recognition-driven assembly of self-limiting supramolecular protein nanoparticles displaying enzymatic activity

Publication year: 2015
Authors: Piccinini E. a, Pallarola D. a, Battaglini F. b, Azzaroni O. *a
Published in: Chemical Communications, 2015, Issue 79, p. 14754-7
We report the recognition-driven assembly of self-limiting protein nanoparticles displaying enzymatic activity. Solution self-assembly of concanavalin A lectin and glycoenzyme glucose oxidase leads to [...]

SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis

Publication year: 2015
Authors: Souto D.E. 1, Fonseca A.M. 2, Barragan J.T. 1, Luz Rde C. 3, Andrade H.M. 2, Damos F.S. 3, Kubota L.T. 4
Published in: Biosensors and Bioelectronics, 2015, Vol. 70, p. 275-81
In this work, an SPR immunosensor was developed to elucidate the reaction kinetics between a protein of unknown function in Leishmania infantum (hypothetical C1 protein) and specific antibodies of [...]

UV crosslinked poly(acrylic acid): a simple method to bio-functionalize electrolyte-gated OFET biosensors

Publication year: 2015
Authors: M. Y. Mulla a, P. Seshadri a, L. Torsi a, K. Manoli a, A. Mallardi b, N. Ditaranto a, M. V. Santacroce c, C. Di Franco c, G. Scamarcio c and M. Magliulo, *a
Published in: Journal of Materials Chemistry B, 2015, Vol. 3, p. 5049-5057
A simple and time-saving wet method to endow the surface of organic semiconductor films with carboxyl functional groups is presented. A thin layer of poly(acrylic acid) (pAA) is spin-coated directly on the electronic channel of [...]

Surface Dynamics Study of DNA Loop Formation Using Surface Plasmon Resonance

Publication year: 2015
Authors: Mary Arugula, Fuling Yang, Subramaniam Somasundaram, Christopher J Easley, Curtis Shannon and Aleksandr Simonian
Published in: ECS Transactions, 2015, Vol. 66, Issue 36, p. 19-29
Electrochemical proximity assay (ECPA), was recently developed for direct protein quantitation as low as 158 fM. In this assay, the loop-like DNA structure holds methylene blue (MB-DNA) in close proximity with the gold surface when target is present, ensuring efficient electron transfer. However, for studying DNA binding [...]

Biosensors for Detecting Genetically Modified Organisms in Food and Feed

Publication year: 2015
Authors: Mary Arugula, Alina Chanysheva, Kiril Vaglenov and Aleksandr Simonian
Published in: ECS Transactions, 2015, Vol. 66, Issue 36, p. 31-38
The detection of genetically modified organisms is of growing interest given their considerable presence in vast variety of food and feed ingredients, as well as their environmental impact. In this study we demonstrate real-time, label- free detection of DNA from genetically modified, Roundup Ready (RR) soybeans by surface plasmon resonance (SPR). [...]

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces

Publication year: 2015
Authors: Zhou L. a, Arugula M.A. a, Easley C. b, Shannon C. b, Simonian A.L. a
Published in: ECS Transactions, 2015, Vol. 69 (38), p. 9-16
With increasing significance of characterization in functional interfaces, a Multi-Parametric/Multimodal Spectroscopy Apparatus was proposed here to enhance the power of interface characterization sensing techniques. [...]

Using QCM and SPR for the Kinetic Evaluation of the Binding Between A New Recombinant Chimeric Protein and Specific Antibodies of the Visceral Leishmaniasis

Publication year: 2015
Authors: Souto D.E., Faria A.R., de Andrade H.M., Kubota L.T.
Published in: Current Protein and Peptide Science, 2015, Vol. 16, p. 782-790
In the present study, the surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate the binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies [...]

Investigation of pH-Induced Protein Conformation Changes by Nanomechanical Deflection

Publication year: 2014
Authors: Thakur G. †, Jiang K. †, Lee D. †, Prashanthi K. †, Kim S. †‡, Thundat T. †
Published in: Langmuir, 2014, Vol. 30 (8), p. 2109–2116
Broad-spectrum biosensing technologies examine sensor signals using biomarkers, such as proteins, DNA, antibodies, specific cells, and macromolecules, based on direct- or indirect-conformational changes. [...]

Grafting hyaluronic acid onto gold surface to achieve low protein fouling in surface plasmon resonance biosensors

Publication year: 2014
Authors: Liu X., Huang R., Su R., Qi W., Wang L., He Z.
Published in: ACS Applied Materials and Interfaces, 2014, Vol. 6(15), p. 13034-42
Antifouling surfaces capable of reducing nonspecific protein adsorption from natural complex media are highly desirable in surface plasmon resonance (SPR) biosensors. A new protein-resistant surface [...]

Effects of Composition of Oligo(ethylene glycol)-Based Mixed Monolayers on Peptide Grafting and Human Immunoglobulin Detection

Publication year: 2014
Authors: Islam N. †, Gurgel P.V. †, Rojas O.J. †‡, Carbonell R.G. †§
Published in: Journal of Physical Chemistry, 2014, Vol. 118 (10), p. 5361–5373
Alkanethiols carrying ethylene glycol units (EGn, n = 3 or 6) with amine termini (EG3NH2 or EG6NH2) were coadsorbed with a “diluent”, hydroxyl-terminated alkanethiol (EG3OH), to form mixed self-assembled monolayers (SAMs). The mixed SAMs were characterized, and hexameric peptide ligand His-Trp-Arg-Gly-Trp-Val (HWRGWV), which shows affinity binding toward the Fc (constant fragment) of human immunoglobulin (IgG), was grafted onto different dilutions of EG6NH2–EG3OH mixed SAMs for preparation of IgG detection surfaces. [...]

An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes

Publication year: 2014
Authors: Ihalainen P. 1, Pettersson F. 2, Pesonen M. 2, Viitala T. 3, Määttänen A.1, Österbacka R. 2, Peltonen J. 1
Published in: Nanotechnology, 2014, Vol. 25, Issue 9
In this study, two different supramolecular recognition architectures for impedimetric detection of DNA hybridization have been formed on disposable paper-supported inkjet-printed gold electrodes. The gold electrodes were fabricated using a gold nanoparticle based ink. The first recognition architecture consists of subsequent layers of biotinylated self-assembly monolayer (SAM), streptavidin and biotinylated DNA probe. [...]

Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance

Publication year: 2014
Authors: Islam N. 1, Shen F. 1, Gurgel P.V. 1, Rojas O.J. 2, Carbonell R.G. 3
Published in: Biosensors and Bioelectronics, Vol. 58, 2014, p. 380-387
This paper characterizes the potential of novel hexameric peptide ligands for on-line IgG detection in bioprocesses. Surface Plasmon Resonance (SPR) was used to study the binding of human IgG to the hexameric peptide ligand HWRGWV, which was covalently grafted to alkanethiol self-assembled monolayers (SAM) on gold surfaces. [...]

Effect of gold nanoparticles on the structure and electron-transfer characteristics of glucose oxidase redox polyelectrolyte-surfactant complexes

Publication year: 2014
Authors: Cortez M.L. a,b, Marmisollé W. a, Pallarola D. b, Pietrasanta L.I. c, Murgida D.H. a, Ceolín M. b, Azzaroni O. b, Battaglini F. a
Published in: Chemistry A European Journal, 2014, Vol. 20, Issue 41, p. 13037–13404
Efficient electrical communication between redox proteins and electrodes is a critical issue in the operation and development of amperometric biosensors. The present study explores the advantages of a nanostructured redox-active polyelectrolyte-surfactant complex containing [Os(bpy)2Clpy](2+) (bpy=2,2'-bipyridine, py= pyridine) as the redox centers and gold nanoparticles (AuNPs) as nanodomains for boosting the electron-transfer propagation throughout the assembled film in the presence of glucose oxidase (GOx). [...]

Label-Enhanced Surface Plasmon Resonance: A New Concept for Improved Performance in Optical Biosensor Analysis

Publication year: 2013
Authors: Niko Granqvist 1,2, Anders Hanning 3, Lars Eng 3, Jussi Tuppurainen 2, Tapani Viitala 1
Published in: Sensors, 2013, Vol. 13(11), p. 15348-15363
Surface plasmon resonance (SPR) is a well-established optical biosensor technology with many proven applications in the study of molecular interactions as well as in surface and material science. SPR is usually applied [...]

Specificity and regenerability of short peptide ligands supported on polymer layers for immunoglobulin G binding and detection

Publication year: 2013
Authors: Zhang Y. 1, Islam N. 2, Carbonell R.G. 2, Rojas O.J. 1,2
Published in: ACS Applied Materials and Interfaces, 2013, Vol.5 (16), p. 8030-7
We demonstrate the specificity, regenerability, and excellent storage stability of short peptide-based systems for detection of immunoglobulin G (IgG). The bioactive component consisted of [...]

Specific binding of immunoglobulin G with bioactive short peptides supported on antifouling copolymer layers for detection in quartz crystal microgravimetry and surface plasmon resonance

Publication year: 2013
Authors: Yanxia Zhang †, Nafisa Islam ‡, Ruben G. Carbonell ‡, Orlando J. Rojas †‡
Published in: Analytical Chemistry, Vol. 85, Issue 2, 2013, p. 1106–1113
A new peptide-based system supported on copolymer brushes grafted from gold sensors and with resistance to nonspecific adsorption is reported for selective binding of human immunoglobulin G (IgG). [...]

Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development

Publication year: 2013
Authors: Ihalainen P. 1, Majumdar H. 2,†, Viitala T. 3, Törngren B. 1, Närjeoja T. 4, Määttänen A. 1, Sarfraz J. 1, Härmä H. 4, Yliperttula M. 3, Österbacka R. 2, Peltonen J. 1
Published in: Biosensors, 2013, Vol. 3, p. 1-17
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRPantibody are grown on printed gold electrodes on disposable paper-substrates. [...]

Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor

Publication year: 2013
Authors: Chang Zeng (a), Xin Huang (a), Jiangmin Xu (a), Guifen Li (a), Jie Ma (a), Hai-Feng Ji (b), Shuifang Zhu (a), Hongjun Chen (a)
Published in: Analytical Biochemistry, Vol. 440 (2013), p. 18–22
We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maizechlorotic mottle virus (MCMV). [...]

Structural and Functional Characteristics of Chimeric Avidins Physically Adsorbed onto Functionalized Polythiophene Thin Films, Albers et al. ACS Applied Materials & Interfaces

Publication year: 2012
Authors: Willem M. Albers †, Jani M. Pelto †, Clèment Suspenè ‡, Juha A.Määttä §, Abderrahim Yassar ⊥, Vesa P. Hytönen §#, Inger M.Vikholm-Lundin †, Kirsi Tappura †
Published in: ACS Applied Materials and Interfaces, 2012, Vol. 4, p. 4067 − 4077
Stabilized bioreceptor layers are of great importance in the design of novel biosensors. In earlier work, chimeric avidins enabled immobilization of biotinylated anti-bodies onto gold surfaces with greater stability compared to more conventional avidins (wild-type avidin and streptavidin). [...]