Publications: Biosensors

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Rapid and Highly Sensitive Detection of Dopamine Using Conjugated Oxaborole-Based Polymer and Glycopolymer Systems

Publication year: 2017
Authors: Jiang K., Wang Y., Thaku G., Kotsuchibashi Y., Naicker S., Narain R., Thundat T.
Published in: ACS Applied Materials & Interfaces, 2017, Vol. 9 (18), p. 15225–15231
A conjugated polymer interface consisting of an oxaborole containing polymer and a glycopolymer was used for achieving very high selectivity in dopamine (DA) detection. [...]

Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils

Publication year: 2017
Authors: Vuoriluoto M. 1, Orelma H. 1, Lundahl M. 1, Borghe M. 1, Rojas O.J.1,2,3
Published in: Biomacromolecules, 2017, Vol.18 (6), p. 1803–1813
We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). [...]

Surface plasmon resonance biosensor for enzymatic detection of small analytes

Publication year: 2017
Authors: Miyazaki C.M., Shimizu F.M., Mejía-Salazar J.R., Oliveira O.N., Ferreira M.
Published in: Nanotechnology, 2017, Vol. 28(14), p. 145501
Surface plasmon resonance (SPR) biosensing is based on the detection of small changes in the refractive index on a gold surface modified with molecular recognition materials, thus being mostly limited to detecting large molecules. [...]

Early-stage detection of VE-cadherin during endothelial differentiation of human mesenchymal stem cells using SPR biosensor

Publication year: 2017
Authors: Fathi F. 1, Rezabakhsh A. 2, Rahbarghazi R. 3, Rashidi M.R. 4
Published in: Biosensors and Bioelectronics, 2017, Vol. 96, p. 358-366
Surface plasmon resonance (SPR) biosensors are most commonly applied for real-time dynamic analysis and measurement of interactions in bio-molecular studies and cell-surface analysis without the need for labeling processes. [...]

Ionic strength-controlled hybridization and stability of hybrids of KRAS DNA single-nucleotides: A surface plasmon resonance study

Publication year: 2017
Authors: Giamblanco N. 1, Petralia S. 2, Conoci S. 3, Messineo C. 4, Marletta G. 4
Published in: Colloids and Surfaces B: Biointerfaces, 2017, Vol. 158, p. 41-46
The discrimination of a fully matched, unlabeled KRAS wild-type (WT) (C-G) target sample with respect to three of the most frequent KRAS codon mutations (G12 S (C-A), G12 R (C-C), G12C (C-T)) was investigated using an [...]

Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer

Publication year: 2017
Authors: Yanchao Mayang 1,2, Xiwen He 1,2, Langxing Chen 1,2, Yukui Zhang 1,2,3
Published in: Microchimica Acta, 2017, Vol. 184 (8), p. 2749–2757
A surface plasmon resonance sensor whose surface has been modified with boronic acid groups was developed in order to bind and detect transferrin. 4-Mercaptophenylboronic acid (4-MPBA) was utilized [...]

Integration of Biorecognition Elements on PEDOT Platforms through Supramolecular Interactions

Publication year: 2017
Authors: Sappia L.D. 1,2, Piccinini E. 3, Marmisollé W. 3, Santilli N. 1, Maza E. 4, Moya S. 4, Battaglini F. 5, Madrid R.E. 1,2, Azzaroni O. 3
Published in: Advanced Materials Interfaces, 2017, 1700502
The rapidly emerging field of organic bioelectronics exploits the functional versatility of conducting polymers to transduce biological recognition events into electronic signals. For the majority of biosensors [...]

Investigation of graphene-on-metal substrates for SPR-based sensor using finite-difference time domain

Publication year: 2017
Authors: Said F. A. 1, Menon P. S. 1, Rajendran V. 2, Shaari S. 1, Majlis B. Y. 1
Published in: IET Nanobiotechnology, 2017, Vol. 11, Issue 8, p. 981 – 986
In this study, the authors investigated the effects of a single layer graphene as a coating layer on top of metal thin films such as silver, gold, aluminum and copper using finite-difference time domain method. [...]

Amine-appended polyaniline as a water dispersible electroactive polyelectrolyte and its integration into functional self-assembled multilayers

Publication year: 2016
Authors: Waldemar A. Marmisollé a, Eliana Mazaa b, Sergio Moya b, Omar Azzaroni a
Published in: Electrochimica Acta, 2016, Vol. 210, p. 435–444
We present an effective and simple method for the synthesis of poly(3-aminobenzylamine) (PABA) using a chemical oxidation strategy in aqueous solution. The polymer was characterized by NMR, LDI-TOF mass spectrometry, UV-visible, XPS and ATR-FTIR spectroscopies. [...]

Kinetic studies of bovine serum albumin interaction with PG and TBHQ using surface plasmon resonance

Publication year: 2016
Authors: Fathi F. a,b,c, Ezzati Nazhad Dolatanbadi J. a, Rashidi M.R. a, Omidi Y. a
Published in: International Journal of Biological Macromolecules, 2016, Vol. 91, p. 1045–1050
Propyl gallate (PG) and tert-butylhydroquinone (TBHQ) are examples of phenolic antioxidant agents, which have widespread uses in food industry. In this study, for the first time, we report on the interaction of PG and TBHQ [...]

Layer-by-layer assembly of functionalized reduced graphene oxide for direct electrochemistry and glucose detection

Publication year: 2016
Authors: Mascagni D.B. a, Miyazaki C.M. b, da Cruz N.C. a, de Moraes M.L. c, Riul A. Jr. d, Ferreira M. b
Published in: Materials Science and Engineering: C, 2016, Vol. 68, p. 739–745
We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. [...]

Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk

Publication year: 2016
Authors: Farka Z. 1, Juřík T. 1, 2, Pastucha M. 1, 2, Skládal P.1, 2
Published in: Analytical Chemistry, 2016, Vol. 88 (23), p. 11830–11836
Contamination of food by pathogenic bacteria has always been a serious threat for human health. The amount of food exports and imports has been increasing in recent years which requires precise food quality control [...]

SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System

Publication year: 2016
Authors: Millone M.A.D. 1, Ramirez E.A. 1, Chain C.Y. 1, Crivaro A. 2, Romanin D. 2, Rumbo M. 2, Docena G. 2, Cocco M.D. 3, Pedano M.L. 3, Fainstein A. 3, Montoya J. 4, Vela M.E. 1, Salvarezza R.C. 1
Published in: Journal of Nanomaterials, Vol. 2016, Article ID 5432656, 9 p.
Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins. These substances typically have low molecular weight, [...]

Incorporation of gold nanoparticles into Langmuir-Blodgett films of polyaniline and montmorillonite for enhanced detection of metallic ions

Publication year: 2016
Authors: A. De Barros a, b, C.J.L. Constantino c, J.R.R. Bortoleto d, N.C. Da Cruz d, M. Ferreira b
Published in: Sensors and Actuators B: Chemical, Vol. 236, 2016, p. 408–417
We report on effects from incorporating gold nanoparticles (AuNPs) into Langmuir-Blodgett (LB) films of emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay (OMt). [...]

HRP-Mimicking DNAzyme-Catalyzed in Situ Generation of Polyaniline To Assist Signal Amplification for Ultrasensitive Surface Plasmon Resonance Biosensing

Publication year: 2016
Authors: Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
Published in: Analytical Chemistry, 2017, Vol. 89 (1), p. 673–680
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. [...]

Adsorption Studies of Organophosphonic Acids on Differently Activated Gold Surfaces

Publication year: 2016
Authors: Katrin Niegelhell †, Simon Leimgruber †‡, Thomas Grießer §, Christian Brandl ∥, Boril Chernev ∥, Robert Schennach ⊥, Gregor Trimmel †, and Stefan Spirk †
Published in: Langmuir, 2016, Vol. 32 (6), p. 1550–1559
In this study, the formation of self-assembled monolayers consisting of three organophosphonic acids (vinyl-, octyl-, and tetradecylphosphonic acid) from isopropanol solutions onto differently activated gold surfaces is studied in situ and in real time using multiparameter surface plasmon resonance (MP-SPR). [...]

Label-free C-reactive protein electronic detection with an electrolyte-gated organic field-effect transistor-based immunosensor

Publication year: 2016
Authors: Magliulo M. 1, De Tullio D. 2, Vikholm-Lundin I. 3, Albers W.M. 4, Munter T. 5, Manoli K. 2, Palazzo G. 2, Torsi L. 2
Published in: Analytical and Bioanalytical Chemistry, 2016, Vol. 408(15), p. 3943-52
In this contribution, we propose a label-free immunosensor, based on a novel type of electrolyte-gated field-effect transistor (EGOFET), for ultrasensitive detection of the C-reactive protein (CRP). [...]

Using QCM and SPR for the Kinetic Evaluation of the Binding Between A New Recombinant Chimeric Protein and Specific Antibodies of the Visceral Leishmaniasis

Publication year: 2015
Authors: Souto D.E., Faria A.R., de Andrade H.M., Kubota L.T.
Published in: Current Protein and Peptide Science, 2015, Vol. 16, p. 782-790
In the present study, the surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate the binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies [...]

Synergy between Polyaniline and OMt Clay Mineral in Langmuir–Blodgett Films for the Simultaneous Detection of Traces of Metal Ions

Publication year: 2015
Authors: de Barros A. 1,2, Ferreira M. 3, Constantino C.J.L. 4, Bortoleto J.R.R. 5, Ferreira M. 2
Published in: ACS, Applied Materials and Interfaces, 2015, Vol. 7 [12], p. 6828–6834
We report on Langmuir–Blodgett (LB) films made with emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay mineral (OMt), where synergy between the components was reached to yield an enhanced performance in detecting trace levels of cadmium (Cd2+), lead (Pb2+) and copper (Cu2+). [...]

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Publication year: 2015
Authors: Miyazaki C.M. 1, Pereira T.P. 1, Tavares Mascagni D.B. 2, de Moraes M.L. 3, Ferreira M. 1
Published in: Materials Science and Engineering: C, 2015, Vol. 58, p. 310–315
In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. [...]