Publications: Biosensors

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Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils

Publication year: 2017
Authors: Vuoriluoto M. 1, Orelma H. 1, Lundahl M. 1, Borghe M. 1, Rojas O.J.1,2,3
Published in: Biomacromolecules, 2017, Vol.18 (6), p. 1803–1813
We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). [...]

Surface plasmon resonance biosensor for enzymatic detection of small analytes

Publication year: 2017
Authors: Miyazaki C.M., Shimizu F.M., Mejía-Salazar J.R., Oliveira O.N., Ferreira M.
Published in: Nanotechnology, 2017, Vol. 28(14), p. 145501
Surface plasmon resonance (SPR) biosensing is based on the detection of small changes in the refractive index on a gold surface modified with molecular recognition materials, thus being mostly limited to detecting large molecules. [...]

Early-stage detection of VE-cadherin during endothelial differentiation of human mesenchymal stem cells using SPR biosensor

Publication year: 2017
Authors: Fathi F. 1, Rezabakhsh A. 2, Rahbarghazi R. 3, Rashidi M.R. 4
Published in: Biosensors and Bioelectronics, 2017, Vol. 96, p. 358-366
Surface plasmon resonance (SPR) biosensors are most commonly applied for real-time dynamic analysis and measurement of interactions in bio-molecular studies and cell-surface analysis without the need for labeling processes. [...]

Ionic strength-controlled hybridization and stability of hybrids of KRAS DNA single-nucleotides: A surface plasmon resonance study

Publication year: 2017
Authors: Giamblanco N. 1, Petralia S. 2, Conoci S. 3, Messineo C. 4, Marletta G. 4
Published in: Colloids and Surfaces B: Biointerfaces, 2017, Vol. 158, p. 41-46
The discrimination of a fully matched, unlabeled KRAS wild-type (WT) (C-G) target sample with respect to three of the most frequent KRAS codon mutations (G12 S (C-A), G12 R (C-C), G12C (C-T)) was investigated using an [...]

Label-free C-reactive protein electronic detection with an electrolyte-gated organic field-effect transistor-based immunosensor

Publication year: 2016
Authors: Magliulo M. 1, De Tullio D. 2, Vikholm-Lundin I. 3, Albers W.M. 4, Munter T. 5, Manoli K. 2, Palazzo G. 2, Torsi L. 2
Published in: Analytical and Bioanalytical Chemistry, 2016, Vol. 408(15), p. 3943-52
In this contribution, we propose a label-free immunosensor, based on a novel type of electrolyte-gated field-effect transistor (EGOFET), for ultrasensitive detection of the C-reactive protein (CRP). [...]

Amine-appended polyaniline as a water dispersible electroactive polyelectrolyte and its integration into functional self-assembled multilayers

Publication year: 2016
Authors: Waldemar A. Marmisollé a, Eliana Mazaa b, Sergio Moya b, Omar Azzaroni a
Published in: Electrochimica Acta, 2016, Vol. 210, p. 435–444
We present an effective and simple method for the synthesis of poly(3-aminobenzylamine) (PABA) using a chemical oxidation strategy in aqueous solution. The polymer was characterized by NMR, LDI-TOF mass spectrometry, UV-visible, XPS and ATR-FTIR spectroscopies. [...]

Kinetic studies of bovine serum albumin interaction with PG and TBHQ using surface plasmon resonance

Publication year: 2016
Authors: Fathi F. a,b,c, Ezzati Nazhad Dolatanbadi J. a, Rashidi M.R. a, Omidi Y. a
Published in: International Journal of Biological Macromolecules, 2016, Vol. 91, p. 1045–1050
Propyl gallate (PG) and tert-butylhydroquinone (TBHQ) are examples of phenolic antioxidant agents, which have widespread uses in food industry. In this study, for the first time, we report on the interaction of PG and TBHQ [...]

Layer-by-layer assembly of functionalized reduced graphene oxide for direct electrochemistry and glucose detection

Publication year: 2016
Authors: Mascagni D.B. a, Miyazaki C.M. b, da Cruz N.C. a, de Moraes M.L. c, Riul A. Jr. d, Ferreira M. b
Published in: Materials Science and Engineering: C, 2016, Vol. 68, p. 739–745
We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. [...]

Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk

Publication year: 2016
Authors: Farka Z. 1, Juřík T. 1, 2, Pastucha M. 1, 2, Skládal P.1, 2
Published in: Analytical Chemistry, 2016, Vol. 88 (23), p. 11830–11836
Contamination of food by pathogenic bacteria has always been a serious threat for human health. The amount of food exports and imports has been increasing in recent years which requires precise food quality control [...]

SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System

Publication year: 2016
Authors: Millone M.A.D. 1, Ramirez E.A. 1, Chain C.Y. 1, Crivaro A. 2, Romanin D. 2, Rumbo M. 2, Docena G. 2, Cocco M.D. 3, Pedano M.L. 3, Fainstein A. 3, Montoya J. 4, Vela M.E. 1, Salvarezza R.C. 1
Published in: Journal of Nanomaterials, Vol. 2016, Article ID 5432656, 9 p.
Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins. These substances typically have low molecular weight, [...]

Incorporation of gold nanoparticles into Langmuir-Blodgett films of polyaniline and montmorillonite for enhanced detection of metallic ions

Publication year: 2016
Authors: A. De Barros a, b, C.J.L. Constantino c, J.R.R. Bortoleto d, N.C. Da Cruz d, M. Ferreira b
Published in: Sensors and Actuators B: Chemical, Vol. 236, 2016, p. 408–417
We report on effects from incorporating gold nanoparticles (AuNPs) into Langmuir-Blodgett (LB) films of emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay (OMt). [...]

HRP-Mimicking DNAzyme-Catalyzed in Situ Generation of Polyaniline To Assist Signal Amplification for Ultrasensitive Surface Plasmon Resonance Biosensing

Publication year: 2016
Authors: Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
Published in: Analytical Chemistry, 2017, Vol. 89 (1), p. 673–680
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. [...]

Adsorption Studies of Organophosphonic Acids on Differently Activated Gold Surfaces

Publication year: 2016
Authors: Katrin Niegelhell †, Simon Leimgruber †‡, Thomas Grießer §, Christian Brandl ∥, Boril Chernev ∥, Robert Schennach ⊥, Gregor Trimmel †, and Stefan Spirk †
Published in: Langmuir, 2016, Vol. 32 (6), p. 1550–1559
In this study, the formation of self-assembled monolayers consisting of three organophosphonic acids (vinyl-, octyl-, and tetradecylphosphonic acid) from isopropanol solutions onto differently activated gold surfaces is studied in situ and in real time using multiparameter surface plasmon resonance (MP-SPR). [...]

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces

Publication year: 2015
Authors: Zhou L. a, Arugula M.A. a, Easley C. b, Shannon C. b, Simonian A.L. a
Published in: ECS Transactions, 2015, Vol. 69 (38), p. 9-16
With increasing significance of characterization in functional interfaces, a Multi-Parametric/Multimodal Spectroscopy Apparatus was proposed here to enhance the power of interface characterization sensing techniques. [...]

Using QCM and SPR for the Kinetic Evaluation of the Binding Between A New Recombinant Chimeric Protein and Specific Antibodies of the Visceral Leishmaniasis

Publication year: 2015
Authors: Souto D.E., Faria A.R., de Andrade H.M., Kubota L.T.
Published in: Current Protein and Peptide Science, 2015, Vol. 16, p. 782-790
In the present study, the surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) techniques were employed to kinetically evaluate the binding affinity of a new recombinant chimeric protein (CP10) toward anti-Leishmania infantum antibodies [...]

Synergy between Polyaniline and OMt Clay Mineral in Langmuir–Blodgett Films for the Simultaneous Detection of Traces of Metal Ions

Publication year: 2015
Authors: de Barros A. 1,2, Ferreira M. 3, Constantino C.J.L. 4, Bortoleto J.R.R. 5, Ferreira M. 2
Published in: ACS, Applied Materials and Interfaces, 2015, Vol. 7 [12], p. 6828–6834
We report on Langmuir–Blodgett (LB) films made with emeraldine salt polyaniline (PAni-ES) and organophilic montmorillonite clay mineral (OMt), where synergy between the components was reached to yield an enhanced performance in detecting trace levels of cadmium (Cd2+), lead (Pb2+) and copper (Cu2+). [...]

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

Publication year: 2015
Authors: Miyazaki C.M. 1, Pereira T.P. 1, Tavares Mascagni D.B. 2, de Moraes M.L. 3, Ferreira M. 1
Published in: Materials Science and Engineering: C, 2015, Vol. 58, p. 310–315
In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. [...]

Recognition-driven assembly of self-limiting supramolecular protein nanoparticles displaying enzymatic activity

Publication year: 2015
Authors: Piccinini E. a, Pallarola D. a, Battaglini F. b, Azzaroni O. *a
Published in: Chemical Communications, 2015, Issue 79, p. 14754-7
We report the recognition-driven assembly of self-limiting protein nanoparticles displaying enzymatic activity. Solution self-assembly of concanavalin A lectin and glycoenzyme glucose oxidase leads to [...]

SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis

Publication year: 2015
Authors: Souto D.E. 1, Fonseca A.M. 2, Barragan J.T. 1, Luz Rde C. 3, Andrade H.M. 2, Damos F.S. 3, Kubota L.T. 4
Published in: Biosensors and Bioelectronics, 2015, Vol. 70, p. 275-81
In this work, an SPR immunosensor was developed to elucidate the reaction kinetics between a protein of unknown function in Leishmania infantum (hypothetical C1 protein) and specific antibodies of [...]

UV crosslinked poly(acrylic acid): a simple method to bio-functionalize electrolyte-gated OFET biosensors

Publication year: 2015
Authors: M. Y. Mulla a, P. Seshadri a, L. Torsi a, K. Manoli a, A. Mallardi b, N. Ditaranto a, M. V. Santacroce c, C. Di Franco c, G. Scamarcio c and M. Magliulo, *a
Published in: Journal of Materials Chemistry B, 2015, Vol. 3, p. 5049-5057
A simple and time-saving wet method to endow the surface of organic semiconductor films with carboxyl functional groups is presented. A thin layer of poly(acrylic acid) (pAA) is spin-coated directly on the electronic channel of [...]