Publications: Biosensors


Monitoring biomolecular interaction between folic acid and bovine serum albumin

Publication year: 2020
Authors: Chilom C.G. a, David M. b, Florescu M. b
Published in: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2020, Vol. 230, p.118074
Folic acid is a bioactive food component whose deficiency can lead to a variety of health problems, while a high intake of folic acid can reduce the cytotoxicity of natural killer cells. The binding mechanism of folic acid to free bovine serum albumin (BSA) was studied using fluorescence, while the biomolecular interaction between confined-BSA and free folic acid was assessed by electrochemical methods and surface plasmon resonance. [...]

Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone

Publication year: 2020
Authors: Riikka Peltomaa a,b,1, Zdeněk Farka a,c, Matthias J. Mickert a,2, Julian C. Brandmeier a, Matěj Pastucha c, Antonín Hlaváček d, Mónica Martínez-Orts e, Ángeles Canales e, Petr Skládal c, Elena Benito-Peña b, María C. Moreno-Bondi b, Hans H. Gorris a
Published in: Biosensors and Bioelectronics, Vol. 170, p. 112683
Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) [...]

Functional Plasma Polymerized Surfaces for Biosensing

Publication year: 2020
Authors: Makhneva* E., Barillas L 1., Farka Z. 2, Pastucha M. 2, Skládal P. 2, Weltmann K.D. 1, Fricke K. 1
Published in: Applied Material Interfaces, 2020, Vol.12, 14, p.17100–17112
The capabilities of biosensors for fast, economic, and user-friendly analysis of complex samples has led to the exploitation of analytical devices for detection, quantification, and monitoring of specific chemical species for various applications. For a sufficiently high surface reactivity toward the adopted bioreceptors, a thin functional layer is required to enable coupling of the target biomolecules and to provide good stability in the presence of a sample matrix.[...]

Enhancing the Sensitivity of Biotinylated Surfaces by Tailoring the Design of the Mixed Self-Assembled Monolayer Synthesis

Publication year: 2020
Authors: Blasi D. ‡†, Sarcina L. ‡§, Tricase A. ‡§, Stefanachi A. ‖, Leonetti F.*‖, Alberga D. $, Mangiatordi* Ͱ, Manoli K. †§, Scamarcio G. ◊∫, Picca R.A. *†§, Torsi L. †§#
Published in: ACS Omega 2020, Vol. 5, 27, p. 16762–16771
Thiolated self-assembled monolayers (SAMs) are typically used to anchor on a gold surface biomolecules serving as recognition elements for biosensor applications. Here, the design and synthesis of N-(2-hydroxyethyl)-3-mercaptopropanamide (NMPA) in biotinylated mixed SAMs is proposed as an alternative strategy with respect to on-site multistep functionalization of SAMs prepared from solutions of commercially available thiols.[...]

Nickel (II) phthalocyanine-tetrasulfonic-Au nanoparticles nanocomposite film for tartrazine electrochemical sensing

Publication year: 2020
Authors: de Lima L. 1, Maciel C. 2, Ferreira A. 1, de Almeida J. 2, Ferreira M. 1*
Published in: Materials Letters, 2020 Vol. 262, p. 127186
In this work, we report the analytical detection of tartrazine through differential pulse voltammetry (DPV) using gold nanoparticles (AuNPs) and Nickel(II)-phthalocyanine-tetrasulfonic (NiTsPc). The Layer-by-Layer (LbL) film was constructed following the Au-(AuNPs-PAH/NiTsPc)5 architecture.[...]

Layer-by-Layer nanostructured films of magnetite nanoparticles and polypyrrole towards synergistic effect on methylparaben electrochemical detection

Publication year: 2020
Authors: de Lima L., Daikuzono C., Miyazaki C., Pereira E., Ferreira M.*
Published in: Applied Surface Science 2020, Vol. 505, p. 144278
The sustained use of cosmetics, hygiene products and medicines containing methylparaben (MeP) can be related to cases of cancer in women and children, which is why it is important to develop simple ways to monitor this compound. Gold electrodes were modified with Layer-by-Layer (LbL) films of magnetite nanoparticles (MNP) and polypyrrole (Ppy) for electrochemical determination of MeP.[...]

An investigation of the synergistic effect between magnetite nanoparticles and polypyrrole in nanostructured layer‐by‐layer films

Publication year: 2020
Authors: de Lima L. 1,2, Maciel C. 3, Ferreira A. 1, Rubira R. 4, Constantino C. 4, Ferreira M. *1 1M.
Published in: Journal of Applied Polymer Science, 2020
In this paper, we report the controlled fabrication of layer‐by‐layer (LbL) films deposited on gold substrates with three different supramolecular architectures using polypyrrole (Ppy) and magnetite nanoparticles (Fe3O4‐np), besides conventional poly(allylamine hydrochloride) (PAH) e poly(vinyl sulfonic acid) (PVS) polyelectrolytes, demonstrating the synergistic effect between Ppy and Fe3O4‐np such as a result of their interaction.[...]

Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor

Publication year: 2020
Authors: Macchia E. 12, Sarcina L. 1, Picca R.A. 1, Manoli K. 1, Di Franco C. 1, Scamarcio G. 34 Torsi L. 125
Published in: Analytical and Bioanalytical Chemistry, 2020, Vol. 412, p. 811–818
Early diagnosis of the infection caused by human immunodeficiency virus type-1 (HIV-1) is vital to achieve efficient therapeutic treatment and limit the disease spreading when the viremia is at its highest level. To this end, a point-of-care HIV-1 detection carried out with label-free, low-cost, and ultra-sensitive screening technologies would be of great relevance.[...]

Assessment of Gold Bio-Functionalization for Wide-Interface Biosensing Platforms

Publication year: 2020
Authors: Sarcina L. 1,Torsi L. 123, Picca R.A. 12, Manoli K. 12*, Macchia E. 3
Published in: Sensors, 2020, Vol. 20, p. 3678
The continuous improvement of the technical potential of bioelectronic devices for biosensing applications will provide clinicians with a reliable tool for biomarker quantification down to the single molecule. Eventually, physicians will be able to identify the very moment at which the illness state begins, with a terrific impact on the quality of life along with a reduction of health care expenses.[...]

Maleic anhydride and acetylene plasma copolymer surfaces for SPR immunosensing

Publication year: 2019
Authors: Makhneva E. 1,2, Farka Z. 3, Pastucha M. 4,5, Obrusník A. 1,2, Horáčková V. 4, Skládal P. 4,5, Zajíčková L. 1,2
Published in: Analytical and Bioanalytical Chemisry, 2019
We report on the successful application of carboxyl-rich plasma polymerized (PP) films as a matrix layer for bioreceptor immobilization in surface plasmon resonance (SPR) immunosensing. Composition and [...]

Handbook of MP-SPR

Publication year: 2019
Authors: Marquart A., Kuncová-Kallio J., Albers M., Bombera R., Ståhlberg R.
Published in: Handbook of MP-SPR, 2019, Vol. 1, Based on fifth edition of Arnoud Marquart's Surface Plasmon Resonance book
Handbook of Multi-Parametric Surface Plasmon Resonance for Molecular Interaction Analysis - Theory and Practice

Ch. 11: Optical and Acoustic Label-free Instrumentation for Molecular Detection with a Focus on Food

Publication year: 2019
Authors: J. Kuncová-Kallio, S. Auer, A. Spehar, H.H. Qu, D. Spasic, J. Lammertyn
Published in: Rapid Antibody-based technologies is Food Analysis, 2019, Vol. 15, p. 223-255
Food Chemistry, Function and Analysis: Optical and Acoustic Label-free Instrumentation for Molecular Detection with a Focus on Food

Refractive Index and Sensing of Glucose Molarities Determined using Au-Cr K-SPR at 670/785 nm Wavelenght

Publication year: 2019
Authors: P. Susthitha Menon*, Budi Mulyanti, Nur Akmar Jamil, Chandra Wulandari, Harbi Setyo Nugroho, Gan Siew Mei, Noor Faizah Zainul Abidin, Lilik Hasanah, Roer Eka Pawinanto & Dilla Duryha Berhanuddin
Published in: Sains Malaysiana 48(6)(2019): 1259–1265

Trypanosoma cruzi Virulence Factors for the Diagnosis of Chagas’ Disease

Publication year: 2019
Authors: Cecilia Yamil Chain,*,† Dênio Emanuel Pires Souto,*,‡ María Laura Sbaraglini,§ Carlos A. Labriola,∥ María Antonieta Daza Millone,† Eduardo Alejandro Ramirez,† José Sebastián Cisneros,† Constanza Lopez-Albizu,⊥ Karenina Scollo,⊥ Lauro T. Kubota,# Andrés Mariano Ruiz,⊥ and María Elena Vela†
Published in: ACS Infectious Diseases
trans-Sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas disease, that have highly antigenic domains in their structure and were reported as potential tools for diagnosis of the illness. [...]

Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules

Publication year: 2019
Authors: Tasbasi BB 1, Guner BC 1, Sudagidan M 1, Ucak S 2, Kavruk M3, Ozalp VC 4.
Published in: Analytical Biochemistry
Lateral flow assay (LFA) type of biosensors have been popular due to cost-effectiveness and easy-interpretation for instant results, most common examples of applications being pregnancy tests, food safety or medical diagnostics. There are several examples of reports with high sensitivity, including pre-concentration of the sample by magnetic pull-down. However, sensitivity and direct detection designs with aptamers has been a limiting factor for developing aptamers-based LFA assays. [...]

A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders

Publication year: 2019
Authors: Ariana de Souza Moraes 1,2,3, Doralina Guimarães Brum3,4, Jéssica Cristiane Magalhães Ierich 1,2,3, Akemi Martins Higa1,2,3, Amanda Stefanie Jabur Assis2,3, Celina Massumi Miyazaki2, Flávio Makoto Shimizu5, Luís Antonio Peroni 6, M. Teresa Machini7, Amilton Antunes Barreira8,9, Marystela Ferreira 2, Osvaldo N. Oliveira Jr.5 & Fabio Lima Leite2,3
Published in: Scientific Reports
A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients’ prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). [...]

The sensitive detection of ODAM by using sandwich-type biosensors with a cognate pair of aptamers for the early diagnosis of periodontal disease

Publication year: 2019
Authors: Lee B.H. 1, Kim S.H. 1, Ko Y. 2, Park J.C. 3, Ji S. 4, Gu M.B. 5
Published in: Biosensors and Bioelectronics, 2019, Vol. 126, p. 122-128
This research aims to develop biosensors which could diagnose periodontal diseases in early phases and predict the illness stage of patients, in order to give them adequate treatment timely. [...]

Analytical Model To Describe the Effect of Polyethylene Glycol on Ionic Screening of Analyte Charges in Transistor-Based Immunosensing

Publication year: 2019
Authors: Haustein N., Gutierrez-Sanz O., Tarasov. A
Published in: ACS Sensors, 2019, Vol. 4, Issue 4, p. 874-882
Recently, the co-immobilization of polyethylene glycol has improved sensor responses of transistor-based immunosensing by approximately three times. However, there is currently [...]

Antibody-mediated biorecognition of myelin oligodendrocyte glycoprotein: computational evidence of demyelination-related epitopes

Publication year: 2019
Authors: Ierich J.C.M. 1,2, Brum D.G. 3, Moraes A.S. 1,2, Higa A.M. 1,2, Garcia P.S. 1,2, Miyazaki C.M. 4, Ferreira M. 4, Peroni L.A. 5, Oliveira G.S. 6, Franca E.F. 6, Freitas L.C.G. 7, Leite F.L. 8
Published in: Scientific Reports, 2019, Vol. 9, Issue 1, p. 2033
Antigen-antibody interaction is crucial in autoimmune disease pathogenesis, as multiple sclerosis and neuromyelitis optica. Given that, autoantibodies are essential [...]

Use of a Branched Linker for Enhanced Biosensing Properties in IgG Detection from Mixed Chinese Hamster Ovary Cell Cultures

Publication year: 2019
Authors: Islam N. a,b, Gurgel P.V. a,c, Rojas O.J. a,d, Carbonell R.G. a,e
Published in: Bioconjugate Chemistry, 2019, Vol. 30, p. 815−825
Tris(2-aminoethyl)-amine (TREN), a branched amine, was coupled to planar surfaces of alkanethiol self-assembled monolayers (SAMs) to increase [...]