Publications: Biosensors

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AuNPs network structures as a plasmonic matrix for ultrasensitive immunoassay based on surface plasmon resonance spectroscopy

Publication year: 2021
Authors: Junjie Hu a, Jialin Zhao a,b, Han Zhu a, Qiang Chen c, Xiaojun Hu a, Kwangnak Koh d, Hongxia Chen a
Published in: Sensors and Actuators B: Chemical, 2021, Vol. 340, p. 129948
Nanoparticle gap, degree of order, and the structure of the nanocomposite functionalized interface have a significant impact on sensing properties. Orderly arranged gold nanoparticles enhance [...]

Specific intracellular binding peptide as sPD-L1 antibody mimic: Robust binding capacity and intracellular region specific modulation upon applied to sensing research

Publication year: 2021
Authors: Junjie Hu a, Zhao-huan Zhang b, Zhongzheng Zhu c, Jie Chen a,d, Xiaojun Hu a, Hongxia Chen a
Published in: Biosensors and Bioelectronics, 2021, Vol. 185, p. 113269
Programmed death ligand 1 (PD-L1) immune checkpoint has been regarded as a new target for predicting cancer immunotherapy. As a transmembrane protein, PD-L1 has very low blood concentration and is likely [...]

Surface Plasmon Resonance Assay for Label-Free and Selective Detection of HIV-1 p24 Protein

Publication year: 2021
Authors: Sarcina L. 1, Mangiatordi G.F. 2, Torricelli F. 3, Bollella P. 1, Gounani Z. 4, Österbacka R. 4, Macchia E. 4, Torsi L. 1,4,5
Published in: Biosensors, 2021, Vol. 11(6), p. 180
The early detection of the human immunodeficiency virus (HIV) is of paramount importance to achieve efficient therapeutic treatment and limit the disease spreading. In this perspective, [...]

One dimensional magneto-optical nanocomplex from silver nanoclusters and magnetite nanorods containing ordered mesocages for sensitive detection of PD-L1

Publication year: 2021
Authors: Xing Huang a,1 Zhao-huan Zhang b,1 Jie Chen a,c, Zhihui Mao a,d, Han Zhu a, Yawen Liu c,d,Zhongzheng Zhu e, Hongxia Chen a
Published in: Biosensors and Bioelectronics, 2021, Vol. 189, p. 113385
Programmed death ligand 1 (PD-L1) is a typical immune checkpoint protein, whose up-regulation on the membrane of different tumor cells inhibits the immune response f T cells and leads to the escape of tumor cells. [...]

Surface Plasmon Resonance Assay for Label-Free and Selective Detection of Xylella Fastidiosa

Publication year: 2021
Authors: Sarcina L. 1, Macchia E. 2, Loconsole G. 3, D'Attoma G. 3, Saldarelli P. 3, Elicio V. 4, Palazzo G. 1, Torsi L. 1,2
Published in: Advanced NanoBiomed Research, 2021, p. 2100043
Xylella fastidiosa is among the most dangerous plant bacteria worldwide causing a variety of diseases, with huge economic impact on agriculture and environment. A surveillance tool, ensuring the highest possible sensitivity [...]

A simple and direct SPR platform combining three-in-one multifunctional peptides for ultra-sensitive detection of PD-L1 exosomes

Publication year: 2021
Authors: Mao Z.a,b, Zhao J.c, Chen J.b,d, Hu X.b, Koh K.e, Chen H.b
Published in: Sensors and Actuators B: Chemical, 2021, Vol.346, p. 130496
Exosomes are lipid-bilayer-enclosed extracellular vehicles (EVs) derived from all cells and circulating in the blood. Identification of cancer specific exosomes in body fluids has shown great potential for [...]

In-situ and real-time probing cellulase biosensor formation and its interaction with lignosulfonate in varied media

Publication year: 2021
Authors: Peipei Wang a,1, Tian Liu a,1, Yena Liu a,1, Jing Tian a, Xinyu Zhang a, Jiaqi Guo a, Yongcan Jin a, Huining Xiao b, Junlong Song a
Published in: Sensors and Actuators B: Chemical, Vol. 329 (2021), 129114
It is a challenge to interpret the signals obtained by Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) accompanied with in the significant changes of the density and/or refractivity of media. [...]

Development of a novel biosensor for Creatine Kinase (CK-MB) using Surface Plasmon Resonance (SPR)

Publication year: 2021
Authors: André L.Ferreira a,1, Lucas F.de Lima b,1, Ariana S.Moraes a,1, Rafael J.G.Rubira c,1, Carlos J.L.Constantino c, Fabio L.Leite a, Adriana O.Delgado-Silva a, Marystela Ferreira a
Published in: Applied Surface Science, Vol. 554 (2021), 149565
In this paper, we report a new biosensor for detection of the cardiac biomarker Creatine Kinase (CK-MB) using Surface Plasmon Surface (SPR) technique, with the purpose of the diagnosis of Acute Myocardial Infarction (AMI). [...]

Combining Chemical Functionalization and FinFET Geometry for Field Effect Sensors as Accessible Technology to Optimize pH Sensing

Publication year: 2021
Authors: D.Rani 1, S.Rollo 1,2, W.Olthuis 2, S.Krishnamoorthy 1, C.P.García 1
Published in: Chemosensors 2021, Vol. 9(2), 20
Field Effect Transistors (FETs) have led the progress of applications measuring the acidity in aqueous solutions thanks to their accuracy, ease of miniaturization and capacity of multiplexing. [...]

Enhancing the Sensitivity of Biotinylated Surfaces by Tailoring the Design of the Mixed Self-Assembled Monolayer Synthesis

Publication year: 2020
Authors: Blasi D. ‡†, Sarcina L. ‡§, Tricase A. ‡§, Stefanachi A. ‖, Leonetti F.*‖, Alberga D. $, Mangiatordi* Ͱ, Manoli K. †§, Scamarcio G. ◊∫, Picca R.A. *†§, Torsi L. †§#
Published in: ACS Omega 2020, Vol. 5, 27, p. 16762–16771
Thiolated self-assembled monolayers (SAMs) are typically used to anchor on a gold surface biomolecules serving as recognition elements for biosensor applications. Here, the design and synthesis of N-(2-hydroxyethyl)-3-mercaptopropanamide (NMPA) in biotinylated mixed SAMs is proposed as an alternative strategy with respect to on-site multistep functionalization of SAMs prepared from solutions of commercially available thiols.[...]

Mild reduction-promoted sandwich aptasensing for simple and versatile detection of protein biomarkers

Publication year: 2020
Authors: Bing Han, Langjian Dong, Lingling Li, Lingjun Sha, Ya Cao*, Jing Zhao*
Published in: Sensors and Actuators B: Chemical, 2020, Vol. 325, p. 128762
Sandwich format has drawn considerable attention in aptasensing in view of simple design, convenient and stable application, and easy coupling with signal amplification strategies; however, developing [...]

Nickel (II) phthalocyanine-tetrasulfonic-Au nanoparticles nanocomposite film for tartrazine electrochemical sensing

Publication year: 2020
Authors: de Lima L. 1, Maciel C. 2, Ferreira A. 1, de Almeida J. 2, Ferreira M. 1*
Published in: Materials Letters, 2020 Vol. 262, p. 127186
In this work, we report the analytical detection of tartrazine through differential pulse voltammetry (DPV) using gold nanoparticles (AuNPs) and Nickel(II)-phthalocyanine-tetrasulfonic (NiTsPc). The Layer-by-Layer (LbL) film was constructed following the Au-(AuNPs-PAH/NiTsPc)5 architecture.[...]

A label-free lead(II) ion sensor based on surface plasmon resonance and DNAzyme-gold nanoparticle conjugates

Publication year: 2020
Authors: Huanan Wu 1, Shuokang Wang 1, Sam Fong Yau Li 2, Qi Bao 1, Qiyong Xu 1
Published in: Analytical and Bioanalytical Chemistry, 2020, Vol. 412, p.7525–7533
Detection of lead(II) (Pb2+) ions in water is important for the protection of human health and environment. The growing demand for onsite detection still faces challenges for sensitive and easy-to-use methods. [...]

Layer-by-Layer nanostructured films of magnetite nanoparticles and polypyrrole towards synergistic effect on methylparaben electrochemical detection

Publication year: 2020
Authors: de Lima L., Daikuzono C., Miyazaki C., Pereira E., Ferreira M.*
Published in: Applied Surface Science 2020, Vol. 505, p. 144278
The sustained use of cosmetics, hygiene products and medicines containing methylparaben (MeP) can be related to cases of cancer in women and children, which is why it is important to develop simple ways to monitor this compound. Gold electrodes were modified with Layer-by-Layer (LbL) films of magnetite nanoparticles (MNP) and polypyrrole (Ppy) for electrochemical determination of MeP.[...]

An investigation of the synergistic effect between magnetite nanoparticles and polypyrrole in nanostructured layer‐by‐layer films

Publication year: 2020
Authors: de Lima L. 1,2, Maciel C. 3, Ferreira A. 1, Rubira R. 4, Constantino C. 4, Ferreira M. *1 1M.
Published in: Journal of Applied Polymer Science, 2020
In this paper, we report the controlled fabrication of layer‐by‐layer (LbL) films deposited on gold substrates with three different supramolecular architectures using polypyrrole (Ppy) and magnetite nanoparticles (Fe3O4‐np), besides conventional poly(allylamine hydrochloride) (PAH) e poly(vinyl sulfonic acid) (PVS) polyelectrolytes, demonstrating the synergistic effect between Ppy and Fe3O4‐np such as a result of their interaction.[...]

Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor

Publication year: 2020
Authors: Macchia E. 12, Sarcina L. 1, Picca R.A. 1, Manoli K. 1, Di Franco C. 1, Scamarcio G. 34 Torsi L. 125
Published in: Analytical and Bioanalytical Chemistry, 2020, Vol. 412, p. 811–818
Early diagnosis of the infection caused by human immunodeficiency virus type-1 (HIV-1) is vital to achieve efficient therapeutic treatment and limit the disease spreading when the viremia is at its highest level. To this end, a point-of-care HIV-1 detection carried out with label-free, low-cost, and ultra-sensitive screening technologies would be of great relevance.[...]

Assessment of Gold Bio-Functionalization for Wide-Interface Biosensing Platforms

Publication year: 2020
Authors: Sarcina L. 1,Torsi L. 123, Picca R.A. 12, Manoli K. 12*, Macchia E. 3
Published in: Sensors, 2020, Vol. 20, p. 3678
The continuous improvement of the technical potential of bioelectronic devices for biosensing applications will provide clinicians with a reliable tool for biomarker quantification down to the single molecule. Eventually, physicians will be able to identify the very moment at which the illness state begins, with a terrific impact on the quality of life along with a reduction of health care expenses.[...]

Monitoring biomolecular interaction between folic acid and bovine serum albumin

Publication year: 2020
Authors: Chilom C.G. a, David M. b, Florescu M. b
Published in: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2020, Vol. 230, p.118074
Folic acid is a bioactive food component whose deficiency can lead to a variety of health problems, while a high intake of folic acid can reduce the cytotoxicity of natural killer cells. The binding mechanism of folic acid to free bovine serum albumin (BSA) was studied using fluorescence, while the biomolecular interaction between confined-BSA and free folic acid was assessed by electrochemical methods and surface plasmon resonance. [...]

Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone

Publication year: 2020
Authors: Riikka Peltomaa a,b,1, Zdeněk Farka a,c, Matthias J. Mickert a,2, Julian C. Brandmeier a, Matěj Pastucha c, Antonín Hlaváček d, Mónica Martínez-Orts e, Ángeles Canales e, Petr Skládal c, Elena Benito-Peña b, María C. Moreno-Bondi b, Hans H. Gorris a
Published in: Biosensors and Bioelectronics, Vol. 170, p. 112683
Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) [...]

Personalized immunoglobulin aptamers for detection of multiple myeloma minimal residual disease in serum

Publication year: 2020
Authors: Claudia Tapia-Alveal, Timothy R. Olsen, Tilla S. Worgall
Published in: Communications Biology, Vol. 3 (2020), 781
Multiple myeloma (MM) is a neoplasm of plasma cells that secrete patient specific monoclonal immunoglobulins. A recognized problem in MM treatment is the early recognition of minimal residual disease (MRD), the major cause of relapse. [...]