Regenerable avidin kit assays Part II: Biomolecule affinity and concentration studies

Application Note #168

 

Binding of different PIPs binding to ORP2.

MP-SPR Navi™ Regenerable avidin kit is an excellent tool in development of MP-SPR based assays. Here, the specific binding affinity of lipid based molecules towards sensor-captured protein was analyzed. Also, concentration analysis of diagnostically relevant biomarkers were performed in human serum samples. Utilizing Regenerable avidin kit reduces time in assa development since there is no need to do traditional immobilization conditions optimization which is typically needed for covalent immobilization over dextran sensor slides.

Recommended MP-SPR instruments for this application:

200_otso_small.jpg 400_KONTIO_LOGO_24012019_cropped.jpg 210_vasa_small.jpg 410A_KAURIS_instrument.jpg  220A_naali_small.jpg 420_ilves_small.jpg
200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 
green-tick.png green-tick.png green-tick.png   green-tick.png green-tick.png

Further reading

  • Take a look at AN#162 to read more about concentration studies and AN#155 of interaction studies.
  • If you are interested in reading more about crude sample measurements, click here.

  • Do you want to find out how to calculate kinetics and affinity out of MP-SPR measurements? Click here.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see MP-SPR instruments comparison? Click here.

  • Here is the original publication.

DOWNLOAD APPLICATION NOTE IN PDF


Back to all Application Notes