Regenerable avidin kit assays Part I: Working principle

Application Note #166

 

Figure 1. MP-SPR Navi™ Regenerable avidin kit principle. The surface can be completely regenerated back to the original biotin-PEG sensor whenever the user wants to change the ligand. Using mild regeneration conditions, only the analyte can be washed off the ligand, enabling next analyte concentration to be loaded.

MP-SPR Navi™ Regenerable avidin kit is an excellent tool in development of MP-SPR based assays. The kit allows the user to conduct precise and high quality measurements in shorter time and convenient experimental set-up. Besides having the ligand available with biotin-conjugation, one does not need to optimize buffers and immobilization conditions. Finally, the cost effectiveness of SPR measurements improves since the same sensor slide can be completely regenerated up to 100 times.

Recommended MP-SPR instruments for this application:

200_otso_small.jpg 400_KONTIO_LOGO_24012019_cropped.jpg 210_vasa_small.jpg 410A_KAURIS_instrument.jpg  220A_naali_small.jpg 420_ilves_small.jpg
200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 

 

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Further reading

  • Take a look at AN#168 to read more about Regenerable avidin kit assay and AN#155 of interaction studies.
  • If you are interested in reading more about crude sample measurements, click here.

  • Do you want to find out how to calculate kinetics and affinity out of MP-SPR measurements? Click here.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see MP-SPR instruments comparison? Click here.

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