Affinity and kinetics of
extracellular vesicles – protein interaction

Application Note #164

 

Figure 2. Affinity and kinetic constants of peptide – extracellular vesicle interaction was determined using TraceDrawer™ for MP-SPR Navi™. The solid black line shows measured data, and the dashed orange line represents the fit to sensogram from sequential injections of three different populations of EVs having A) 1%, B) 5%, and C) 10% coverage of α3β1 integrin.

Extracellular vesicles (EVs) are extensively studied as potential therapeutics, drug delivery systems and for non-invasive diagnostics of diseases, such as cancer. Early detection of ovarian cancer was explored using Multi-Parametric Surface Plasmon Resonance (MP-SPR) instrument. Tumor EVs (SKOV-3) were loaded onto surface-immobilized LXY30 peptide. Affinity and kinetic constants of the interaction, bound mass and layer thickness
were determined based on MP-SPR measurement. LXY30 peptide exhibits a high affinity to EVs expressing α3β1 integrin and offers possibility for diagnostic applications.

Recommended MP-SPR instruments for this application:

200_otso_small.jpg 400_KONTIO_LOGO_24012019_cropped.jpg 210_vasa_small.jpg 410A_KAURIS_instrument.jpg  220A_naali_small.jpg 420_ilves_small.jpg
200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 
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Further reading:

  • See also AN#156 on extracellular vesicles uptake by cells, AN#152  on interactions of nanoparticles and AN#151 about measurement of soft and hard corona on nanoparticle in 100% serum.
  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see MP-SPR instruments comparison? Click here.

  • Here is the original publication.

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