Virus interaction studies using MP-SPR

Application Note # 145

Figure 1. Peptides PolyK-SIINFEKL and SIINFEKL interaction with oncolytic adenoviruses (OAds).

To develop cancer vaccines, a virus-peptide interaction was measured with Multi-Parametric Surface Plasmon Resonance (MP-SPR). Viruses were coated with peptides in order to increase virus immunogenicity. Oncolytic adenoviruses (OAds) were attached on the sensor surface. Tumor-specific major histocompatibility complex (MHC) peptides attachment to the OAds was measured in real-time. Modified positive polyK-SIINFEKL peptide bound to the virus surface (binding constant 2.88 x 10 M), whereas unmodified neutral SIINFEKL peptide did not bind.

Recommended instrument for this application:

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200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 
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Further reading

  • You can read more about lipid layers in AN#139, small molecules interacting with protein in AN#144 or small molecules interacting with with living cells in AN#137.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see comparison MP-SPR instruments? Click here.

  • Here are a few publications you can have a look at. The original publication can be found here.

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