Supported lipid layers characterization with MP-SPR

Application Note #139

Langmuir-blodgett

Figure 2. Change in SPR peak minimum angle during lipid deposition, From left to right: SLB formation on SiO2 surface, SLV formation on PEG-SAM surface and SLB formation on 6kDa dextran surface.

Multi-Parametric Surface Plasmon Resonance (MP-SPR) was used to characterize lipid layer structures adsorbed on a different substrates. Silicon dioxide and low molecule weight dextran surfaces supported formation of lipid bilayer (SLB) whereas thiolated polyethylene glycol supported vesicular layer formation (SLV). Thickness and refractive index of deposited lipid layer was calculated: SLB was about 5nm thick layer whereas SLV was 10nm thick layer.

Recommended instrument for this application:

200_otso_small.jpg 400_KONTIO_LOGO_24012019_cropped.jpg 210_vasa_small.jpg 410A_KAURIS_instrument.jpg  220A_naali_small.jpg 420_ilves_small.jpg
200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 
green-tick.png    green-tick.png   green-tick.png   

Further reading

  • If you are interested in lipids, we recommend to have a look at AN#157 about detection of toxin induced pore formation in membranes and AN#151 about measurement of soft and hard corona on liposomes.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see comparison of MP-SPR instruments? Click here.

  • Here are a few publications you can have a look at.

DOWNLOAD APPLICATION NOTE IN PDF


Back to all Application Notes