Antibody and antigen interaction measured with MP-SPR

Application Note #138

Langmuir-blodgett


Figure 3.
Anti-HSA binding to the immobilized HSA with four different concentration. Injection is started at time point zero and arrow is showing ending point. Calculated binding constants for the interaction is presented in the table.

Human serum albumin antibody (anti-HSA) interaction with immobilized human serum albumin (HSA) was measured using Multi-Parametric Surface Plasmon Resonance (MP-SPR). As assumed, commercial anti-HSA binds strongly to the HSA and dissociates extremely slowly from the HSA. Steady state binding as well as kinetic of the binding was calculated for the interaction.

Recommended instrument for this application:

200_otso_small.jpg 400_KONTIO_LOGO_24012019_cropped.jpg 210_vasa_small.jpg 410A_KAURIS_instrument.jpg  220A_naali_small.jpg 420_ilves_small.jpg
200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 
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Further reading

  • If you are interested in antibody - protein interactions, we recommend to have a look at AN#147 about PureKinetics™ and AN#155 about faster interaction measurements using KineticTitration.

  • Do you want to see how MP-SPR instruments work? Click here.

  • Do you want to see comparison MP-SPR instruments? Click here.

  • Here are a few publications you can have a look at.

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