Drug interaction with cell monolayer measured with MP-SPR

Application Note #137

Langmuir-blodgett

Figure 1. MDCKII cell monolayer was deposited on a gold sensor slide. Drug interaction with the cell monolayer was measured in constant flow conditions.

Cell monolayer was deposited on a SPR sensor slide. MultiParametric Surface Plasmon Resonance (MP-SPR) was used to measure drugs (propranolol and D-mannitol) interaction with the cell monolayer in real time and in controlled flow conditions. It was possible to distinguish between paracellular and transcellular drug absorption routes.

Recommended instrument for this application:

200_otso_small.jpg 400_KONTIO_LOGO_24012019_cropped.jpg 210_vasa_small.jpg 410A_KAURIS_instrument.jpg  220A_naali_small.jpg 420_ilves_small.jpg
200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 
green-tick.png  green-tick.png green-tick.png  green-tick.png green-tick.png   

Further reading

  • If you are interested in living cell studie,s you may also view AN#156 about nanoparticle uptake by living cells and AN#154 about cell adhesion on implant material surface and cancer cell detection.

  • Do you want to see how MP-SPR instruments work? Click here.
  • Do you want to see comparison of MP-SPR instruments? Click here.
  • Here are a few publications you can have a look at.

DOWNLOAD APPLICATION NOTE IN PDF


Back to all Application Notes