Determine biofunctional layer binding capacity with MP-SPR.
Immunosensor development.

Application Note #132

Langmuir-blodgett


Figure 3
. (A) MP-SPR signal response after injecting 1–20 μg/mL bio-CRP antigen over MuOH:Biotin-PEG-thiol (85:15 mol%)/streptavidin sensor surface.

MP-SPR is an effective reference tool for immunosensor development.

MP-SPR is utilized to determine affinity biosensor surface binding capacity of different parts of the immunosensor. Streptavidin binding capacity to the MBP-thiol SAM was 366 ± 2 ng/cm2 and Bio-CRP antigen binding capacity to the MBP-thiol /streptavidin surface was 105 ng/cm2. The MP-SPR also allowed for concentration optimization for the sensor system.

Recommended instrument for this application:

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200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 
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Further reading

  • If you are interested in biosensor development, see also AN#117 about detection of DNA and AN#160 about bacteria detection from powdered milk.

  • Do you want to find out more about MP-SPR theory? Click here.

  • Do you want to see comparison of MP-SPR instruments? Click here.

  • Here are a few publications you can have a look at.

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