Real-time characterization of polyelectrolyte multilayers
with MP-SPR

Application Note #111

polyelectrolyte measurement


Figure 3
. SPR curve of pure gold sensor slide in buffer (black curve), and curves with increasing number of PE-layers from left to right (orange curves; first PEI layer, after that alternating PSS and PDADMAC).

Multi-Parametric Surface Plasmon Resonance (MP-SPR) was utilized for precise real-time characterization of polyelectrolyte multilayer build-up. Not only kinetics of adsorption was monitored but also thickness of the each deposited layer was calculated. Thickness of the layers increased exponentially from 1.5nm.

Polyelectrolyte multilayers (PEM) are specific examples of Layer-byLayer (LbL) nanofabrications, where well-defined nanoscale film structures are built on surfaces by selfassembly. PEMs are formed by sequential deposition of oppositely charged macromolecules that bind to each other by electrostatic interactions (Fig1). The layer buildup is dependent on many parameters, such as temperature, pH, ionic strength of the solutions, and the type of materials used for deposition, and these can also be used to control the structure and properties of the forming layer [1,2]. Materials like nanoparticles, carbon nanotubes, light, pH or temperature responsive polymers can be used to build PEMs with desired functionalities or properties. There are a multitude of applications of PEMs in optics, sensing, filtration, coating, composite materials and drug delivery.

Recommended instrument for this application:

200_otso_small.jpg 400_KONTIO_LOGO_24012019_cropped.jpg 210_vasa_small.jpg 410A_KAURIS_instrument.jpg  220A_naali_small.jpg 420_ilves_small.jpg
200 OTSO 400 KONTIO 210A VASA  410A KAURIS  220A NAALI 420A ILVES 
     green-tick.png    green-tick.png  

Further reading

  • If you are interested in polyelectrolytes, see also AN#128 about thickness
    and refractive index of polyelectrolytes and AN#159 about polymer swelling in vapors.

  • Do you want to find out how to calculate thickness out of MP-SPR measurements? Click here.

  • Do you want to see MP-SPR instruments range? Click here.

  • Here are a few publications you can have a look at.

DOWNLOAD APPLICATION NOTE IN PDF


Back to all Application Notes