Publications: Application Notes


Application Notes

AN#169 Diagnostic method for serological testing of covid-19 antibodies

Considering the indisputable need for Covid-19 related diagnostic tools, we have developed a dedicated serological testing method based on MP-SPR technology. [...]

AN#168 Regenerable avidin kit assays Part II: Biomolecule affinity and concentration studies

This application note introduces the MP-SPR Navi™ Regenerable avidin kit, which allows to the use of same sensor for multiple different targets and ligands up to 100 regeneration cycles. [...]

AN#167 In-situ capture of T-cells and analysis of membrane receptor affinity with biofunctionalized lipid sensor

Multi-Parametric Surface Plasmon Resonance (MP-SPR) was applied for characterization of interaction of T-cell receptors (TCR) from tumor-specific CD8+ T-cells. Intact live cells were captured onto biomimetic surfaces composed of artificial cell membranes functionalized with peptide-major histocompatibility complexes (pMHC). Real-time affinity analysis of immunological synapse formation between TCR and pMHC was completed from 4 different T-cell populations.

AN#166 Regenerable avidin kit assays Part I: Working principle

This Application Note introduces the working principle of MP-SPR Navi™ Regenerable avidin kit, which allows to use the same sensor for multiple capture of biotinylated ligands. [...]

AN#165 Cellulose fiber-based yarn development for capturing estrogen residues from aqueous matrices with MP-SPR

This application note introduces preparation of cellulose based material for hormone capture with MP-SPR...

AN#164 Affinity and kinetics of extracellular vesicles – protein interaction

Extracellular vesicles (EVs) are extensively studied as potential therapeutics, drug delivery systems and for non-invasive diagnostics of diseases, such as cancer. Early detection of ovarian cancer was explored using Multi-Parametric Surface Plasmon Resonance (MP-SPR) instrument. Tumor EVs (SKOV-3) were loaded onto surface-immobilized LXY30 peptide.[...]

AN#163 Characterization of micrometer thick layers of spin-coated chitin

Multi-Parametric Surface Plasmon Resonance (MP-SPR) instruments can measure optical properties and address surface interactions from very thin films (down to Ångstroms) up to micrometer thick layers. The measurement of thicker sensor coatings is based on a SPR waveguide mode. [...]

AN#162 Concentration analysis of nutritionally important proteins from milk

Concentrations of proteins were determined from milk and milk-based infant formula using Multi-Parametric Surface Plasmon Resonance (MP-SPR) instrument. [...]

AN#161 Polymerization kinetics and morphology changes of thermo-responsive polymer

Multi-Parametric Surface Plasmon Resonance (MP-SPR) measures surface changes in real-time. High sensitivity and minute volumes make the measurement [...]

AN#160 Biosensor for bacteria detection from powdered milk

Contamination of food by pathogenic bacteria is a serious threat to human health and thus biosensors for fast and accurate food quality control are extensively studied. [...]

AN#159 Material swelling studies using MP-SPR: Cellulose in water vapor and polymer barrier in solvent vapors

Multi-Parametric Surface Plasmon Resonance (MP-SPR) detects interaction of vapors with different materials in real-time and without help of any labels. As the method works with thin films and minute volumes, [...]

AN#158 Predicting cellulose nanocrystals dispersibility using MP-SPR

Cellulose nanocrystals (CNCs) are rod-shaped nanomaterials utilized in biomedical devices, emulsion stabilizers, rheological modifiers and in various other composite applications. [...]

AN#157 Detection of toxin induced pore formation in membranes

Pore forming toxins are a protein family which interacts with the cell membrane causing hemolytic pores that are lethal for their target cells. In water the proteins are firmly folded but upon interaction with the cell membrane lipids, [...]

AN#156 Nanoparticle uptake by cells measured using MP-SPR

Nanoparticles (NPs) are extensively studied as drug delivery systems. NPs enter the cells usually by active transport, i.e. endocytosis. Multi-Parametric Surface Plasmon Resonance (MP-SPR) is previously used for [...]

AN#155 Faster interaction measurements using MP-SPR KineticTitration

KineticTitration significantly reduces time required to run an assay with different concentrations. It is also useful for interactions that are difficult to regenerate or when regeneration damages the ligand on the surface. In the measurement, analyte samples are flown over the surface [...]

AN#154 Real-time cancer cell detection and cell adhesion on implant materials surface

Implants are covered with proteins and cells when introduced into the body. To understand interactions on these interfaces, in vitro tools are utilized. Real-time label-free platforms allowing dynamic [...]

AN#153 Metal-organic framework (MOF) characterization using MP-SPR

Metal-polyphenol complex (MPC) film deposition was monitored in situ using Multi-Parametric Surface Plasmon Resonance (MP-SPR). The sequential deposition of Tannic Acid (TA) and Fe(III) ions was performed [...]

AN#152 Drug delivery nanocarrier studies using MP-SPR

Nanocarriers are being extensively studied as drug delivery systems for cancer and gene therapy amongst others. Block copolymeric micelles (LMP) were developed as mucoadhesive ocular drug delivery vehicles. [...]

AN#151 MP-SPR measurements of soft and hard corona on nanoparticle in 100% serum

When nanocarriers are introduced into the blood circulation, they are rapidly covered with a protein corona, which is a complex layer of biomolecules such as proteins, lipids, and other plasma components. [...]

AN#150 Organophosphonates characterization using MP-SPR

Phosphonic acids adsorption behavior was studied using Multi-Parametric Surface Plasmon Resonance (MP-SPR). Tetradecylphosphonic acid (TPA) adsorption onto differently treated gold surfaces was measured. [...]

AN#149 Polymer characterization using MP-SPR - Adsorption studies and layer thickness

Polymers are extensively studied and often interactions play a key role in the product functionality. Two separate polymer interaction studies were conducted using label-free real-time Multi-Parametric Surface Plasmon Resonance (MP-SPR). [...]

AN#147 Analyzing dissociation kinetics of IgG from protein A using MP-SPR and PureKinetics™

IgG dissociation kinetics from immobilized Protein A was studied using real-time Multi-Parametric Surface Plasmon Resonance (MP-SPR). Various dissociation buffers were tested to determine the most efficient solution. [...]

AN#146 Atomic-scale resolution graphene layer characterization in air using MP-SPR

Chemical-vapour-deposition (CVD)-grown graphene films were characterized with non-invasive Multi-Parametric Surface Plasmon Resonance (MP-SPR). Both layer thickness and refractive index were obtained simultaneously [...]

AN#145 Virus interaction studies using MP-SPR

To develop cancer vaccines, a virus-peptide interaction was measured with Multi-Parametric Surface Plasmon Resonance (MP-SPR). Viruses were coated with peptides in order to increase virus immunogenicity. [...]

AN#144 Small molecular weight drug-protein interaction measured with MP-SPR

The interaction of a small molecular weight drug (indomethacin) to Human serum albumin (HSA) was measured with label-free Multi-Parametric Surface Plasmon Resonance (MP-SPR). HSA was attached to a sensor surface with amine coupling chemistry. [...]

AN#143 Real-time monitoring of antibody binding by parallel MP-SPR, potentiometry and impedance spectroscopy

Multi-Parametric Surface Plasmon Resonance (MP-SPR) equipped with an electrochemistry (EC) flow-cell was used to run potentiometric and impedance measurements in parallel with the MP-SPR measurement.[...]

AN#142 Real-time monitoring of metal stripping and deposition with EC-MP-SPR

Multi-Parametric Surface Plasmon Resonance (MP-SPR) enables combined electrochemical and SPR measurements (EC-MP-SPR). In this study, MP-SPR was utilized to follow real-time copper deposition and stripping processes under constant current (galvanostatic) conditions. [...]

AN#141 In vitro monitoring of drug release kinetics with real-time MP-SPR

A real-time Multi-Parametric Surface Plasmon Resonance (MP-SPR) instrument was used to monitor the release rate of a small molecular weight drug, Perphenazine, from thin EUDRAGIT®-based polymer films. The release kinetics was clearly faster [...]

AN#140 Self-assembly of gold nanoparticles measured with MP-SPR

Gold nanoparticles (AuNPs) exhibits interesting optical and electronic properties that find application in sensors, catalysis, electronics, photonics, solar cells, cancer diagnosis and therapy. Controlled attachment of Au nanoparticles at a solid interface is required for many of these applications. [...]

AN#139 Supported lipid layers characterization with MP-SPR

Supported lipid membranes can be used in vitro experiments to mimic biological barriers, such as cell membranes. It is also possible to incorporate other molecules, like receptors or membrane proteins into the lipid layer structure to mimic specific functionality of the biological membrane. [...]

AN#138 Antibody and antigen interaction measured with MP-SPR

Protein - antibody interactions are extremely important research area for pharmaceutical industry and protein research. Antibody-based biotechnology drugs have been shown to be effective treatments to several obstinate diseases. Multi-Parametric Surface Plasmon Resonance (MP-SPR) is sensitive tool to determine molecule interactions. [...]

AN#137 Drug interaction with cell monolayer measured with MP-SPR

In vitro cell assays are widely used during drug discovery. Traditionally these assays needs labelled materials and the analysis is based on post-detection with e.g. UV-, fluorescence or mass spectroscopy. Multi-Parametric SPR enables real time measurements [...]

AN#136 Polymer collapse due to pH or electric potential change monitored with MP-SPR

Conductive polymers (CP) are utilized in drug delivery systems, microelectronics, photonics and also for material in biomedical engineering. CP polymer poly (3,4-ethylenedioxythiophene) (PEDOT) was deposited on a sensor slide and above that Poly (acrylic acid) (PAA) brush which is a promising biointerface material. [...]

AN#133 Characterization of atomic layer deposited metal films and nanolaminates

Miniaturization of optics, electronics and photovoltaics is an on-going process in todays industries. For effective application and manufacturing development is highly important that the materials can be characterized effectively. Characterization of nanoscale metal layers [...]

AN#132 Determine biofunctional layer binding capacity with MP-SPR. Immunosensor development.

MP-SPR is utilized to determine affinity biosensor surface binding capacity of different parts of the immunosensor. When developing new type of biosensors, it is usually of prime importance to evaluate the performance against an established method. Surface plasmon resonance has been utilized for biosensor studies [...]

AN#130 SPR Navi™ Immobilizer MP-SPR

SPR Navi™ Immobilizer is compatible with the SPR Navi™ sensor slides and SPR Navi™ sensor slide holder. The immobilizer forms two channels (1 µL each), which copy the shape of the SPR Navi™ standard flow-cell. The SPR Navi™Immobilizer [...]

AN#129 Comparing QCM & MP-SPR. A flowrate study.

The difference in the hydrodynamic flow conditions for SPR and QCM measurement chambers is often disregarded when SPR andQCM data is used in combination. This causes non-definable differences between the data from these two sources. [...]

AN#128 Determine layer unique refractive index and thickness with MP-SPR

Multi-Parametric Surface Plasmon Resonance (MP-SPR) can be used to determine an unique thickness and a refractive index (RI) of ultrathin films without prior knowledge of the RI or the thickness of the layer. Polyelectrolyte multilayer was measured using MP-SPR two wavelength method. [...]

AN#127 Thin film deposition MP-SPR

Thin film deposition is a growing field ranging from chemical vapor deposition (CVD), atomic layer deposition (ALD), sputtering, spin coating, Langmuir-Blodgget deposition and others. MP-SPR provides a unique tool for measurements of ultrathin (0.5-5 nm) and thin (5-100 nm) films. Unlike other techniques, MP-SPR is [...]

AN#126 Protein interaction with cellulose MP-SPR

Nanocellulose is a promising new type of material for point-of-care diagnostic devices and other low cost consumer analysis devices. The prevention of non-specific adsorption of biomolecules is a key to detection sensitivity of the system and evaluation of its suitability for give application. Hence, a BSA adsorption study was performed. [...]

AN#125 Binding kinetics of antibodies MP-SPR

PKCε (protein kinase Cε) is involved in diseases such as cancer, type II diabetes and Alzheimer's disease. Therefore, inhibitors and activators of PKCε are promising for research and drug development. Llama single chain antibodies (VHHs) are a new category of monoclonal antibodies that can specifically activate or inhibit human PKCε. [...]

AN#120 Electrochemical SPR of copper electrodeposition

Electrochemistry can be utilized as a detection method or driving force for chemical reactions. Combining electrochemistry with SPR can be utilized in monitoring electrochemical reactions in situ, or it can be used as an complementary detection method to provide additional informationa about the reaction. [...]

AN#117 Quantitative detection of DNA MP-SPR

dNA assays can be applied for detecting genetic material in order to detect genetic disorders, mutations, gene transfection or species from a large variety of samples. By assaying specific single stranded oligonucleotides of over 20 nucleotides in length, it is possible to detect and quantify [...]

AN#116 Characterization of graphene oxide films with MP-SPR

Single-sheet graphene (SG) has high electrical and thermal conduction and high tensile strength. Due to its electrical properties, optical transparency and good chemical resistance, it is a promising material to replace indium tin oxide (ITO) and fluorine tin oxide (FTO) in solar cells and LEDs. [...]

AN#115 Polymer layers MP-SPR & QCM-D

Amphiphilic polymers tend to self assemble and create organized structures. This property is used to prepare functional surfaces, such as those with tailored surface wetting, lubrication and colloidal stability. These in turn are used in drug delivery, emulsifiers in paints etc. [...]

AN#112 In situ monitoring of metal-organic framework assembly with MP-SPR

Metal organic frameworks (MOFs) are crystalline supramolecular assemblies. MOFs have high potential for hydrogen storage, drug delivery, gas sensing, optics and catalysis. A MOF composed of copper and benzenetricarboxylic acid was synthesized and characterized in situ using MP-SPR method. [...]

AN#111 Real-time characterization of polyelectrolyte multilayers with MP-SPR

Polyelectrolyte multilayers (PEMs) are soft nanoscale films that are built by layer-by-layer (LbL) assembly in solution on a substrate. Materials such as nanoparticles, carbon nanotubes, light-, pH- or temperature-active polymers are used to develop functional materials and coatings. [...]