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In situ analysis of liposome hard and soft protein corona structure and composition in a single label-free workflow

Publication year: 2020
Authors: Kari O. *ab, Ndika J. c, Parkkila P. b, Louna A. a, Lajunen T. ǂa, Puustinen A. d, Viitala T. *§b, Alenius H. ce, Urtti A. af
Published in: Nanoscale, 2020, Vol. 12, p. 1728
Methodological constraints have limited our ability to study protein corona formation, slowing nanomedicine development and their successful translation into the clinic. We determined hard and soft corona structural properties along with the corresponding proteomic compositions on liposomes in a label-free workflow: surface plasmon resonance and a custom biosensor for in situ structure determination on liposomes and corona separation, and proteomics using sensitive nanoliquid chromatography tandem mass spectrometry with open-source bioinformatics platforms.[...]

Nickel (II) phthalocyanine-tetrasulfonic-Au nanoparticles nanocomposite film for tartrazine electrochemical sensing

Publication year: 2020
Authors: de Lima L. 1, Maciel C. 2, Ferreira A. 1, de Almeida J. 2, Ferreira M. 1*
Published in: Materials Letters, 2020 Vol. 262, p. 127186
In this work, we report the analytical detection of tartrazine through differential pulse voltammetry (DPV) using gold nanoparticles (AuNPs) and Nickel(II)-phthalocyanine-tetrasulfonic (NiTsPc). The Layer-by-Layer (LbL) film was constructed following the Au-(AuNPs-PAH/NiTsPc)5 architecture.[...]

Surfactant Interactions with Protein-Coated Surfaces: Comparison between Colloidal and Macroscopically Flat Surfaces

Publication year: 2020
Authors: Mateos H. 1,*, Valentini A. 2, Lopez F. 3, Palazzo G. 4
Published in: Biomimetics 2020, Vol. 5(3), p. 31
Surface interactions with polymers or proteins are extensively studied in a range of industrial and biomedical applications to control surface modification, cleaning, or biofilm formation. In this study we compare surfactant interactions with protein-coated silica surfaces differing in the degree of curvature (macroscopically flat and colloidal nanometric spheres). The interaction with a flat surface was probed by means of surface plasmon resonance (SPR) while dynamic light scattering (DLS) was used to study the interaction with colloidal SiO2 (radius 15 nm).Surface interactions with polymers or proteins are extensively studied in a range of industrial and biomedical applications to control surface modification, cleaning, or biofilm formation. In this study we compare surfactant interactions with protein-coated silica surfaces differing in the degree of curvature (macroscopically flat and colloidal nanometric spheres). The interaction with a flat surface was probed by means of surface plasmon resonance (SPR) while dynamic light scattering (DLS) was used to study the interaction with colloidal SiO2 (radius 15 nm).[...]

Layer-by-Layer nanostructured films of magnetite nanoparticles and polypyrrole towards synergistic effect on methylparaben electrochemical detection

Publication year: 2020
Authors: de Lima L., Daikuzono C., Miyazaki C., Pereira E., Ferreira M.*
Published in: Applied Surface Science 2020, Vol. 505, p. 144278
The sustained use of cosmetics, hygiene products and medicines containing methylparaben (MeP) can be related to cases of cancer in women and children, which is why it is important to develop simple ways to monitor this compound. Gold electrodes were modified with Layer-by-Layer (LbL) films of magnetite nanoparticles (MNP) and polypyrrole (Ppy) for electrochemical determination of MeP.[...]

Delineating the Molecular Basis of the Calmodulin–bMunc13-2 Interaction by Cross-Linking/Mass Spectrometry—Evidence for a Novel CaM Binding Motif in bMunc13-2

Publication year: 2020
Authors: Piotrowski C. 1, Moretti R. 2, Ihling C. 1,Haedicke A. 3†, Liepold T. 4, Lipstein N. 5, Meiler J. 2, Jahn O. 4,*, Sinz A. 1,*
Published in: Cells, 2020, Vol.9 (1) , p.136
Exploring the interactions between the Ca2+ binding protein calmodulin (CaM) and its target proteins remains a challenging task. Members of the Munc13 protein family play an essential role in short-term synaptic plasticity, modulated via the interaction with CaM at the presynaptic compartment. [...]

An investigation of the synergistic effect between magnetite nanoparticles and polypyrrole in nanostructured layer‐by‐layer films

Publication year: 2020
Authors: de Lima L. 1,2, Maciel C. 3, Ferreira A. 1, Rubira R. 4, Constantino C. 4, Ferreira M. *1 1M.
Published in: Journal of Applied Polymer Science, 2020
In this paper, we report the controlled fabrication of layer‐by‐layer (LbL) films deposited on gold substrates with three different supramolecular architectures using polypyrrole (Ppy) and magnetite nanoparticles (Fe3O4‐np), besides conventional poly(allylamine hydrochloride) (PAH) e poly(vinyl sulfonic acid) (PVS) polyelectrolytes, demonstrating the synergistic effect between Ppy and Fe3O4‐np such as a result of their interaction.[...]

Single-vesicle imaging reveals lipid-selective and stepwise membrane disruption by monomeric α-synuclein

Publication year: 2020
Authors: Hannestad J. a, Rocha S. b, Agnarsson B. a, Zhdanov V. ac, Wittung-Stafshede P. *b, Höök F. *a
Published in: Proceedings of National Academy of Sciences PNAS, 2020, Vol. 117, 25, p.14178-14186
The interaction of the neuronal protein α-synuclein with lipid membranes appears crucial in the context of Parkinson’s disease, but the underlying mechanistic details, including the roles of different lipids in pathogenic protein aggregation and membrane disruption, remain elusive. Here, we used single-vesicle resolution fluorescence and label-free scattering microscopy to investigate the interaction kinetics of monomeric α-synuclein with surface-tethered vesicles composed of different negatively charged lipids.[...]

Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor

Publication year: 2020
Authors: Macchia E. 12, Sarcina L. 1, Picca R.A. 1, Manoli K. 1, Di Franco C. 1, Scamarcio G. 34 Torsi L. 125
Published in: Analytical and Bioanalytical Chemistry, 2020, Vol. 412, p. 811–818
Early diagnosis of the infection caused by human immunodeficiency virus type-1 (HIV-1) is vital to achieve efficient therapeutic treatment and limit the disease spreading when the viremia is at its highest level. To this end, a point-of-care HIV-1 detection carried out with label-free, low-cost, and ultra-sensitive screening technologies would be of great relevance.[...]

Label-free characterization and real-time monitoring of cell uptake of extracellular vesicles

Publication year: 2020
Authors: Koponen A.ab, Kerkelä E. a, Rojalin T. b, Lázaro-Ibáñez E. b, Suutari T. b, Saari H.b, Siljander P.c, Yliperttula M. b, Laitinen S. a, Viitala T. b
Published in: Biosensors and Bioelectronics, 2020, Vol. 168, p. 112510
Extracellular vesicles (EVs) have the ability to function as molecular vehicles and could therefore be harnessed to deliver drugs to target cells in diseases such as cancer. The composition of EVs determines their function as well as their interactions with cells, which consequently affects the cell uptake efficacy of EVs.[...]

Assessment of Gold Bio-Functionalization for Wide-Interface Biosensing Platforms

Publication year: 2020
Authors: Sarcina L. 1,Torsi L. 123, Picca R.A. 12, Manoli K. 12*, Macchia E. 3
Published in: Sensors, 2020, Vol. 20, p. 3678
The continuous improvement of the technical potential of bioelectronic devices for biosensing applications will provide clinicians with a reliable tool for biomarker quantification down to the single molecule. Eventually, physicians will be able to identify the very moment at which the illness state begins, with a terrific impact on the quality of life along with a reduction of health care expenses.[...]

Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44

Publication year: 2020
Authors: Amorim S.abc, Pashkuleva I. ab, Reis C. defg, Reis R.abc, Pires R. abc
Published in: Journal of Materilals Chemistry, 2020, issue 17
We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) – a multi-signaling biomolecule whose bioactivity depends on its molecular weight.

Heparin/Collagen Surface Coatings Modulate the Growth, Secretome and Morphology of Human Mesenchymal Stromal Cell Response to Interferon‐Gamma

Publication year: 2020
Authors: Cifuentes S. 1, Priyadarshani P. 23,Castilla‐Casadiego D. 4, Mortensen L. 23,Almodóvar J. 4, Domenech M. 15
Published in: Journal of Biomedical Materials Research, 2020
The therapeutic potential of human mesenchymal stromal cells (h‐MSC) is dependent on the viability and secretory capacity of cells both modulated by the culture environment. Our previous studies introduced heparin and collagen I (HEP/COL) alternating stacked layers as a potential substrate to enhance the secretion of immunosuppressive factors of h‐MSCs. [...]

Acceleration of biofilm formation in start-up of sequencing batch biofilm reactor using carriers immobilized with Pseudomonas stutzeri strain XL-2

Publication year: 2020
Authors: Zhang P., Ding X., Zhao B. ⁎, An Q., Guo J.S.
Published in: Bioresource Technology, 2020, vol. 314
P. stutzeri strain XL-2 initially immobilized on polypropylene carriers accelerated the biofilm formation in startup of sequencing batch biofilm reactor (SBBR) (denoted R1). The biofilm formation in R1 was approximately completed in 36 days, which was shorter than that of 48 days in an identical SBBR (denoted R2) without strain XL-2.P. stutzeri strain XL-2 initially immobilized on polypropylene carriers accelerated the biofilm formation in startup of sequencing batch biofilm reactor (SBBR) (denoted R1). The biofilm formation in R1 was approximately completed in 36 days, which was shorter than that of 48 days in an identical SBBR (denoted R2) without strain XL-2.P. stutzeri strain XL-2 initially immobilized on polypropylene carriers accelerated the biofilm formation in startup of sequencing batch biofilm reactor (SBBR) (denoted R1). The biofilm formation in R1 was approximately completed in 36 days, which was shorter than that of 48 days in an identical SBBR (denoted R2) without strain XL-2.[...]

Partitioning of Catechol Derivatives in Lipid Membranes: Implications for Substrate Specificity to Catechol-O-methyltransferase

Publication year: 2020
Authors: Parkkila P., Viitala T.
Published in: ACS Chemical Neuroscience, 2020, Vol.11, 6, p. 969–978
We have utilized multiparametric surface plasmon resonance and impendance-based quartz crystal microbalance instruments to study the distribution coefficients of catechol derivatives in cell model membranes. Our findings verify that the octanol–water partitioning coefficient is a poor descriptor of the total lipid affinity for small molecules which show limited lipophilicity in the octanol–water system.[...]

Diffusion and Protein Corona Formation of Lipid-Based Nanoparticles in the Vitreous Humor: Profiling and Pharmacokinetic Considerations

Publication year: 2020
Authors: Tavakoli S 1., Kari O.K. 1, Turunen T. 1, Lajunen T. 1, Schmitt M. 1, Lehtinen J. 1, Tasaka F. 2, Parkkila P. 1, Ndika J. 3, Viitala T. 1, Alenius H. 3,5, Urtti, A. 1,6,7 Subriz A. *7
Published in: Molecular Pharmaceutics, 2020
The vitreous humor is the first barrier encountered by intravitreally injected nanoparticles. Lipid-based nanoparticles in the vitreous are studied by evaluating their diffusion with single-particle tracking technology and by characterizing their protein coronae with surface plasmon resonance and high-resolution proteomics. Single-particle tracking results indicate that the vitreal mobility of the formulations is dependent on their charge.[...]

Monitoring biomolecular interaction between folic acid and bovine serum albumin

Publication year: 2020
Authors: Chilom C.G. a, David M. b, Florescu M. b
Published in: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2020, Vol. 230, p.118074
Folic acid is a bioactive food component whose deficiency can lead to a variety of health problems, while a high intake of folic acid can reduce the cytotoxicity of natural killer cells. The binding mechanism of folic acid to free bovine serum albumin (BSA) was studied using fluorescence, while the biomolecular interaction between confined-BSA and free folic acid was assessed by electrochemical methods and surface plasmon resonance. [...]

Following in Situ the Degradation of Mesoporous Silica in Biorelevant Conditions: At Last, a Good Comprehension of the Structure Influence

Publication year: 2020
Authors: Bindini E. 1,2, Chehadi Z. 1, Faustini M. 1, Albouy P. 3, Grosso D. 4, Cattoni A. 2, Chanéac C. 1, Azzaroni O. 5, Sanchez C. 1, Boissiè C. 1
Published in: ACS Applied Materials and Interfaces, 2020, Vol.12, p.13598–13612
Mesoporous silica nanoparticles (MSNs) have seen a fast development as drug delivery carriers thanks to their tunable porosity and high loading capacity. The employ of MSNs in biomedical applications requires a good understanding of their degradation behavior both to control drug release and to assess possible toxicity issues on human health.[...]

Synergy between Zwitterionic Polymers and Hyaluronic Acid Enhances Antifouling Performance

Publication year: 2019
Authors: Yinqiang Xia,† Vahid Adibnia,‡ Cancan Shan,† Renliang Huang,§ Wei Qi,†,# Zhimin He,† Guojun Xie,∥ Mateusz Olszewski,∥ Gregory De Crescenzo,⊥ Krzysztof Matyjaszewski,*,∥ Xavier Banquy,*,‡ and Rongxin Su*,†,#
Published in: Langmuir
Challenges associated with nonspecific adsorption of proteins on sensor surfaces have steered the development of novel antifouling materials and strategies. Inspired by human synovial fluid composition and structure, we designed synergistic antifouling coatings with mixtures of hyaluronic acid (HA) and a zwitterionic bottlebrush polymer (BB). [...]

Handbook of MP-SPR

Publication year: 2019
Authors: Marquart A., Kuncová-Kallio J., Albers M., Bombera R., Ståhlberg R.
Published in: Handbook of MP-SPR, 2019, Vol. 1, Based on fifth edition of Arnoud Marquart's Surface Plasmon Resonance book
Handbook of Multi-Parametric Surface Plasmon Resonance for Molecular Interaction Analysis - Theory and Practice

Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-co-glycolic Acid Nanoparticles for Oral Colon Targeting

Publication year: 2019
Authors: Mohamed A. Akl,†,‡ Alma Kartal-Hodzic,† Teemu Suutari,† Timo Oksanen,† Isabella Monia Montagner,§ Antonio Rosato,§,∥ Hatem R. Ismael,‡ Mohsen I. Afouna,‡ Paolo Caliceti,⊥ Marjo Yliperttula,†,⊥ Ahmed M. Samy,‡ Francesca Mastrotto,⊥ Stefano Salmaso,⊥ and Tapani Viitala*,†,#
Published in: ASC Omega
The exploitation of curcumin for oral disease treatment is limited by its low solubility, poor bioavailability, and low stability. Surface-functionalized poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) have shown promising results to ameliorate selective delivery of drugs to the gastrointestinal tract. [...]