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Comparative Characterization of Plasmodium falciparum Hsp70-1 Relative to E. coli DnaK Reveals the Functional Specificity of the Parasite Chaperone

Publication year: 2020
Authors: Lebepe C. 1, Matambanadzo P. 1, Makhoba X. 2, Achilonu I. 3, Zininga T. 1,4, Shonhai A. 1,*
Published in: Biomolecules 2020, Vol. 10(6), p. 856
Hsp70 is a conserved molecular chaperone. How Hsp70 exhibits specialized functions across species remains to be understood. Plasmodium falciparum Hsp70-1 (PfHsp70-1) and Escherichia coli DnaK are cytosol localized molecular chaperones that are important for the survival of these two organisms. [...]

PEDOT-polyamine composite films for bioelectrochemical platforms -flexible and easy to derivatize

Publication year: 2020
Authors: Sappia L. +a, Piccinini E. +a, von Binderling C. a, Knoll W. bcd, Marmisollé W. *a, Azzorini O. *acd
Published in: Material Sciece and Engineering: C, 2020, Vol. 109, p.110575
We report a straightforward route for the preparation of flexible, electrochemically stable and easily functionalizable poly(3,4-ethylenedioxythiophene) (PEDOT) composite films deposited on PET foils as biosensing platforms. For this purpose, poly(allylamine) hydrochloride (PAH) was blended with PEDOT to provide amine-bearing sites for further biofunctionalization as well as to improve the mechanical properties of the films.[...]

It takes two to tango - The case of thebaine 6-O-demethylase

Publication year: 2020
Authors: Kachhap S. a*, Wojdyla Z. a*, Komorek P. a, Kluza A. a, Kurpiewska K. ab, Jachimska B. a,Borowski T.a
Published in: nternational Journal of Biological Macromolecules, 2020, Vol. 163, p. 718-729
Thebaine 6-O-demethylase (T6ODM) is an Fe(II)/2-oxoglutarate-dependent dioxygenase catalysing two oxidative O-demethylation reactions in morphine biosynthesis. Its crystal structure revealed a large active site pocket which is at least two times larger than necessary to accommodate a substrate (thebaine or oripavine) molecule.[...]

Surface Preconditioning Influences the Antifouling Capabilities of Zwitterionic and Nonionic Polymer Brushes

Publication year: 2020
Authors: Víšová I. 1, Vrabcová M. 1, Forinová M. 1, Zhigunová Y. 1, Mironov V. 1, Houska . 1, Bittrich E. 2, Eichhorn K. 2, Hashim H. 3,4, Schovánek P. 1,5, Dejneka A. 1, Vaisocherová - Lísalová H. 1
Published in: Langmuir, 2020, Vol. 36, 29, 8485–8493
Polymer brushes not only represent emerging surface platforms for numerous bioanalytical and biological applications but also create advanced surface-tethered systems to mimic real-life biological processes. In particular, zwitterionic and nonionic polymer brushes have been intensively studied because of their extraordinary resistance to nonspecific adsorption of biomolecules (antifouling characteristics) as well as the ability to be functionalized with bioactive molecules.[...]

Label-Free Analysis with Multiple Parameters Separates G Protein-Coupled Receptor Signaling Pathways

Publication year: 2020
Authors: Temu Suutari*, Sabrina N. Rahman, Henry F. Vischer, Dick van Iperen, Arto Merivaara, Marjo Yliperttula, Rob Leurs, Jeroen Kool, Tapani Viitala*
Published in: Analytical Chemistry, 2020, Vol. 92, 21, p. 14509–14516
Real-time label-free techniques are used to profile G protein-coupled receptor (GPCR) signaling pathways in living cells. However, interpreting the label-free signal responses is challenging, [...]

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

Publication year: 2020
Authors: Víšová I., Smolková B., Uzhytchak M., Vrabcová M., Zhigunova Y., Houska M. ,Surman F., de los Santos Pereira A.,Lunov O., Dejneka A., Vaisocherová‐Lísalová H.
Published in: Macromolecular Bioscience, 2020, Vol. 20, 3, p. 1900351
Ultra‐low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra‐low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied.[...]

Asymmetric flow field-flow fractionation coupled to surface plasmon resonance detection for analysis of therapeutic proteins in blood serum

Publication year: 2020
Authors: Mats Leeman 1, Willem M. Albers 2, Radoslaw Bombera 2, Johana Kuncova-Kallio 2, Jussipekka Tuppurainen 2, Lars Nilsson 3
Published in: Analytical and Bioanalytical Chemistry, 2020
Coupling of surface plasmon resonance (SPR) detection to asymmetric flow field-flow fractionation (AF4) offers the possibility to study active fractions of bio-separations on real samples, such as [...]

Generic High-Capacity Electrochemical Capture and Release of Proteins by Polyelectrolyte Brushes

Publication year: 2020
Authors: Ferrand-Drake del Castillo G., Adali Z., L. N. Hailes R., Xiong K., Dahlin A.*
Published in: Preprint in Chem RXIV, 2020
We present a polyelectrolyte brush electrode with high-capacity for protein immobilization that captures and releases proteins by an electrochemical potential. Central to our concept is the use of polyelectrolyte brushes that switch reversibly by electrochemistry.[...]

Functional Plasma Polymerized Surfaces for Biosensing

Publication year: 2020
Authors: Makhneva* E., Barillas L 1., Farka Z. 2, Pastucha M. 2, Skládal P. 2, Weltmann K.D. 1, Fricke K. 1
Published in: Applied Material Interfaces, 2020, Vol.12, 14, p.17100–17112
The capabilities of biosensors for fast, economic, and user-friendly analysis of complex samples has led to the exploitation of analytical devices for detection, quantification, and monitoring of specific chemical species for various applications. For a sufficiently high surface reactivity toward the adopted bioreceptors, a thin functional layer is required to enable coupling of the target biomolecules and to provide good stability in the presence of a sample matrix.[...]

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Publication year: 2020
Authors: Dedisch S. 12, Wiens A. 2, Davari M.D. 2, Söder D. 1, Rodriguez‐Emmenegger C. 13, Jakob F. 12, Schwaneberg U.*12
Published in: Biotechnology and Bioengineering, 2020, Vol. 117, Issue1, p. 49-61
Enzyme immobilization is extensively studied to improve enzyme properties in catalysis and analytical applications. Here, we introduce a simple and versatile enzyme immobilization platform based on adhesion‐promoting peptides, namely Matter‐tags. Matter‐tags immobilize enzymes in an oriented way as a dense monolayer.[...]

Enhancing the Sensitivity of Biotinylated Surfaces by Tailoring the Design of the Mixed Self-Assembled Monolayer Synthesis

Publication year: 2020
Authors: Blasi D. ‡†, Sarcina L. ‡§, Tricase A. ‡§, Stefanachi A. ‖, Leonetti F.*‖, Alberga D. $, Mangiatordi* Ͱ, Manoli K. †§, Scamarcio G. ◊∫, Picca R.A. *†§, Torsi L. †§#
Published in: ACS Omega 2020, Vol. 5, 27, p. 16762–16771
Thiolated self-assembled monolayers (SAMs) are typically used to anchor on a gold surface biomolecules serving as recognition elements for biosensor applications. Here, the design and synthesis of N-(2-hydroxyethyl)-3-mercaptopropanamide (NMPA) in biotinylated mixed SAMs is proposed as an alternative strategy with respect to on-site multistep functionalization of SAMs prepared from solutions of commercially available thiols.[...]

Self-assembled hybrid nanocomposite films of carbon dots and hydrolyzed collagen

Publication year: 2019
Authors: Tarciane da S. Pinto a, Priscilla N.S. Rodrigues a, Luiz E.S. Marinho a, Rodrigo M. Verly a, J.P. Bretas Roa a, Luiz C.A. de Oliveira b, Fabiano V. Pereira b, Mariana T.Q. de Magalhães c,d, João P. de Mesquita a
Published in: Materials Chemistry and Physics, 2019, Vol. 230, p. 44-53
In the present work, hydrophilic carbon dots (CD) prepared by cotton cellulose were used in the building of self-assembled films with hydrolyzed collagen (CL) to verify their properties, especially molecular interactions and morphology. [...]

Handbook of MP-SPR

Publication year: 2019
Authors: Marquart A., Kuncová-Kallio J., Albers M., Bombera R., Ståhlberg R.
Published in: Handbook of MP-SPR, 2019, Vol. 1
Handbook of Multi-Parametric Surface Plasmon Resonance for Molecular Interaction Analysis - Theory and Practice

Interface Interaction Behavior of Self-Terminated Oligomer Electrode Additives for a Ni-Rich Layer Cathode in Lithium-Ion Batteries: Voltage and Temperature Effects

Publication year: 2019
Authors: Fu-Ming Wang,*,†,‡,§,∥ Tibebu Alemu,† Nan-Hung Yeh,† Xing-Chun Wang,† Yi-Wen Lin,† Chun-Chuan Hsu,† Yung-Jen Chang,† Chia-Hao Liu,† Chiao-I Chuang,† Li-Hao Hsiao,† Jin-Ming Chen,⊥ Shu-Chih Haw,⊥ Wei-Ling Chen,† Quoc-Thai Pham,† and Chia-Hung Su
Published in: ASC Applied Materials and Interfaces
Self-terminated oligomer additives synthesized from bismaleimide and barbituric acid derivatives improve the safety and performance of lithium-ion batteries (LIBs). [...]

The sensitive detection of ODAM by using sandwich-type biosensors with a cognate pair of aptamers for the early diagnosis of periodontal disease

Publication year: 2019
Authors: Lee B.H. 1, Kim S.H. 1, Ko Y. 2, Park J.C. 3, Ji S. 4, Gu M.B. 5
Published in: Biosensors and Bioelectronics, 2019, Vol. 126, p. 122-128
This research aims to develop biosensors which could diagnose periodontal diseases in early phases and predict the illness stage of patients, in order to give them adequate treatment timely. [...]

Layer-by-Layer nanostructured films of magnetite nanoparticles and polypyrrole towards synergistic effect on methylparaben electrochemical detection

Publication year: 2019
Authors: Lucas F. de Lima, Cristiane M. Daikuzono, Celina M. Miyazaki, Elisabete A. Pereira, Marystela Ferreira
Published in: Applied Surface Science
The sustained use of cosmetics, hygiene products and medicines containing methylparaben (MeP) can be related to cases of cancer in women and children, which is why it is important to develop simple ways to monitor this compound.

ORP2 interacts with phosphoinositides and controls the subcellular distribution of cholesterol

Publication year: 2019
Authors: Koponen A. a, Arora A. a, Takahashi K. b, Kentala H. a, Kivelä A.M. a, Jääskeläinen E. a, Peränen J. b, Somerharju P. c, Ikonen E. b, Viitala T. d, Olkkonen V.M. a,b
Published in: Biochimie, 2019, Vol. 158, p. 90-101
ORP2 is a sterol-binding protein with documented functions in lipid and glucose metabolism, Akt signaling, steroidogenesis, cell adhesion, migration and proliferation. [...]

Analytical Model To Describe the Effect of Polyethylene Glycol on Ionic Screening of Analyte Charges in Transistor-Based Immunosensing

Publication year: 2019
Authors: Haustein N., Gutierrez-Sanz O., Tarasov. A
Published in: ACS Sensors, 2019, Vol. 4, Issue 4, p. 874-882
Recently, the co-immobilization of polyethylene glycol has improved sensor responses of transistor-based immunosensing by approximately three times. However, there is currently [...]

Design and evaluation of a multiplexed angular-scanning surface plasmon resonance system employing line-laser optics and CCD detection in combination with multi-ligand sensor chips

Publication year: 2019
Authors: Lakayan D. a, Tuppurainen J. c, Suutari T.E. a,d, van Iperen D.J. e, Somsen G.W. a, Kool J. a
Published in: Sensors and Actuators B: Chemical, 2019, Vol. 282, p. 243-250
An angle-scanning Kretschmann configuration SPR instrument allowing multiplexed analysis is presented. Laser light was guided through optics that converted the collimated light into a line-shaped beam, which was [...]

Antibody-mediated biorecognition of myelin oligodendrocyte glycoprotein: computational evidence of demyelination-related epitopes

Publication year: 2019
Authors: Ierich J.C.M. 1,2, Brum D.G. 3, Moraes A.S. 1,2, Higa A.M. 1,2, Garcia P.S. 1,2, Miyazaki C.M. 4, Ferreira M. 4, Peroni L.A. 5, Oliveira G.S. 6, Franca E.F. 6, Freitas L.C.G. 7, Leite F.L. 8
Published in: Scientific Reports, 2019, Vol. 9, Issue 1, p. 2033
Antigen-antibody interaction is crucial in autoimmune disease pathogenesis, as multiple sclerosis and neuromyelitis optica. Given that, autoantibodies are essential [...]