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Poly(l-lactide-co-2-(2-methoxyethoxy)ethyl methacrylate): A biodegradable polymer with protein resistance

Publication year: 2014
Authors: Jinbao Xua (a), Xuelin Fan (b), Jinxian Yang (b), Chunfeng Ma (a),Xiaodong Ye (b), Guangzhao Zhanga,(b)
Published in: Colloids and Surfaces B: Biointerfaces, Vol. 116 (2014), p. 531–536
We have synthesized poly(l-lactide-co-2-(2-methoxyethoxy)ethyl methacrylate) (LA-co-MEO2MA) containing both degradable and protein resistant units via hybrid copolymerization with(1-tert-butyl-4,4,4-tris(dimethylamino)-2,2-bis[tris(dimethylamino)phophoranylidenamino]-2Λ5, Λ5-catenadi(phosphazene) (t-BuP4) as the catalyst. [...]

Study of the interactions of proteins with a solid surface using complementary acoustic and optical techniques

Publication year: 2014
Authors: Diaconu G1, Schäfer T1
Published in: Biointerphases 9, 029015 (2014)
Membrane water treatment processes suffer severely from (bio)fouling phenomena, defined as an undesired deposition and build-up of adsorbed materials, which alters the membrane performance. [...]

Triggering Protein Adsorption on Tailored Cationic Cellulose Surfaces

Publication year: 2014
Authors: Mohan T. 1, Niegelhell K. 4, Pinto Zarth C.S. 2, Kargl R. 3, Köstler S. 5, Ribitsch V. 1, Heinze T. 2, Spirk S. 1,4, Stana-Kleinschek K. 3
Published in: Biomacromolecules, 2014, Vol. 15 (11), p. 3931–3941
The equipment of cellulose ultrathin films with BSA (bovine serum albumin) via cationization of the surface by tailor-made cationic celluloses is described. In this way, matrices for controlled protein deposition are created, whereas the extent of protein affinity to these surfaces is controlled by the charge density and solubility of the tailored cationic cellulose derivative. [...]

Grafting hyaluronic acid onto gold surface to achieve low protein fouling in surface plasmon resonance biosensors

Publication year: 2014
Authors: Liu X., Huang R., Su R., Qi W., Wang L., He Z.
Published in: ACS Applied Materials and Interfaces, 2014, Vol. 6(15), p. 13034-42
Antifouling surfaces capable of reducing nonspecific protein adsorption from natural complex media are highly desirable in surface plasmon resonance (SPR) biosensors. A new protein-resistant surface [...]

Affibody conjugation onto bacterial cellulose tubes and bioseparation of human serum albumin

Publication year: 2014
Authors: Hannes Orelma,*a Luis O. Morales,a Leena-Sisko Johansson,a Ingrid C. Hoeger,b Ilari Filpponen,a Cristina Castro,c Orlando J. Rojasab and Janne Lainea
Published in: RSC Adv., 2014,4, p. 51440-51450
We attached anti-human serum albumin (anti-HSA) affibody ligands on bacterial cellulose (BC) by EDC–NHS-mediated covalent conjugation and physical adsorption and demonstrate their application for tubular biofiltration of blood proteins. [...]

Modification of surfaces with adsorption of amphiphilic polymers

Publication year: 2014
Authors: Katri Kontturi
Published in: Doctoral Dissertation
In this work the adsorption of amphiphilic polymers and their utilization in the modification of planar surfaces were examined. The approach was to systematically explore adsorption of very different types of [...]

Investigation of pH-Induced Protein Conformation Changes by Nanomechanical Deflection

Publication year: 2014
Authors: Thakur G. †, Jiang K. †, Lee D. †, Prashanthi K. †, Kim S. †‡, Thundat T. †
Published in: Langmuir, 2014, Vol. 30 (8), p. 2109–2116
Broad-spectrum biosensing technologies examine sensor signals using biomarkers, such as proteins, DNA, antibodies, specific cells, and macromolecules, based on direct- or indirect-conformational changes. [...]

Using gelatin protein to facilitate paper thermoformability

Publication year: 2014
Authors: A. Khakalo a, I. Filpponen a, L.S. Johansson a, A. Vishtal b, A.R. Lokanathan a,O. J. Rojas a,c, J. Laine a
Published in: Reactive & Functional Polymers, 2014
One of the main challenges of fiber-based packaging materials is the relatively poor elongation of cellulose under stress, which limits formability and molding in related products. [...]

Effects of Composition of Oligo(ethylene glycol)-Based Mixed Monolayers on Peptide Grafting and Human Immunoglobulin Detection

Publication year: 2014
Authors: Islam N. †, Gurgel P.V. †, Rojas O.J. †‡, Carbonell R.G. †§
Published in: Journal of Physical Chemistry, 2014, Vol. 118 (10), p. 5361–5373
Alkanethiols carrying ethylene glycol units (EGn, n = 3 or 6) with amine termini (EG3NH2 or EG6NH2) were coadsorbed with a “diluent”, hydroxyl-terminated alkanethiol (EG3OH), to form mixed self-assembled monolayers (SAMs). The mixed SAMs were characterized, and hexameric peptide ligand His-Trp-Arg-Gly-Trp-Val (HWRGWV), which shows affinity binding toward the Fc (constant fragment) of human immunoglobulin (IgG), was grafted onto different dilutions of EG6NH2–EG3OH mixed SAMs for preparation of IgG detection surfaces. [...]

Biodegradable Polyurethane Carrying Antifoulants for Inhibition of Marine Biofouling

Publication year: 2014
Authors: Jielin Ma †, Chunfeng Ma *†, Yun Yang †, Wentao Xu †, and Guangzhao Zhang *†‡
Published in: Industrial & Engineering Chemistry Research, 2014, 53 (32), p. 12753–12759
Biodegradable polyurethane with N-(2,4,6-trichlorophenyl)maleimide (TCPM) pendant groups has been prepared via a combination of a thiol–ene click reaction and a condensation reaction. [...]

An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes

Publication year: 2014
Authors: Ihalainen P. 1, Pettersson F. 2, Pesonen M. 2, Viitala T. 3, Määttänen A.1, Österbacka R. 2, Peltonen J. 1
Published in: Nanotechnology, 2014, Vol. 25, Issue 9
In this study, two different supramolecular recognition architectures for impedimetric detection of DNA hybridization have been formed on disposable paper-supported inkjet-printed gold electrodes. The gold electrodes were fabricated using a gold nanoparticle based ink. The first recognition architecture consists of subsequent layers of biotinylated self-assembly monolayer (SAM), streptavidin and biotinylated DNA probe. [...]

Tuning Cellulose Nanocrystal Gelation with Polysaccharides and Surfactants

Publication year: 2014
Authors: Zhen Hu, Emily D. Cranston, Robin Ng, and Robert Pelton*
Published in: Langmuir, 2014, 30 (10), pp 2684–2692
Gelation of cellulose nanocrystal (CNC) dispersions was measured as a function of the presence of four nonionic polysaccharides. Addition of hydroxyethyl cellulose (HEC), hydroxypropyl guar (HPG), or locust bean gum (LBG) to CNC dispersions [...]

Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance

Publication year: 2014
Authors: Islam N. 1, Shen F. 1, Gurgel P.V. 1, Rojas O.J. 2, Carbonell R.G. 3
Published in: Biosensors and Bioelectronics, Vol. 58, 2014, p. 380-387
This paper characterizes the potential of novel hexameric peptide ligands for on-line IgG detection in bioprocesses. Surface Plasmon Resonance (SPR) was used to study the binding of human IgG to the hexameric peptide ligand HWRGWV, which was covalently grafted to alkanethiol self-assembled monolayers (SAM) on gold surfaces. [...]

Surface modification of cellulose nanocrystals with cetyltrimethylammonium bromide

Publication year: 2014
Authors: Tiffany Abitbol, Heera Marway and, Emily D. Cranston Tiffany Abitbol (, Heera Marway (, Emily D. Cranston (
Published in: Special Issue: NANOCELLULOSE; Nordic Pulp & Paper Research Journal Vol 29 no (1) 2014
Cellulose nanocrystals (CNCs) prepared by sulfuric acid hydrolysis of cotton were surface modified with cetyltrimethylammonium bromide (CTAB). Essentially, the counterions of the CNC surface sulfate ester groups are exchanged for cetyltrimethyl-ammonium (CTA+), which acts as a bulky, amphiphilic cation. [...]

Specific binding of immunoglobulin G with bioactive short peptides supported on antifouling copolymer layers for detection in quartz crystal microgravimetry and surface plasmon resonance

Publication year: 2013
Authors: Yanxia Zhang †, Nafisa Islam ‡, Ruben G. Carbonell ‡, Orlando J. Rojas †‡
Published in: Analytical Chemistry, Vol. 85, Issue 2, 2013, p. 1106–1113
A new peptide-based system supported on copolymer brushes grafted from gold sensors and with resistance to nonspecific adsorption is reported for selective binding of human immunoglobulin G (IgG). [...]

Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development

Publication year: 2013
Authors: Ihalainen P. 1, Majumdar H. 2,†, Viitala T. 3, Törngren B. 1, Närjeoja T. 4, Määttänen A. 1, Sarfraz J. 1, Härmä H. 4, Yliperttula M. 3, Österbacka R. 2, Peltonen J. 1
Published in: Biosensors, 2013, Vol. 3, p. 1-17
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRPantibody are grown on printed gold electrodes on disposable paper-substrates. [...]

Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor

Publication year: 2013
Authors: Chang Zeng (a), Xin Huang (a), Jiangmin Xu (a), Guifen Li (a), Jie Ma (a), Hai-Feng Ji (b), Shuifang Zhu (a), Hongjun Chen (a)
Published in: Analytical Biochemistry, Vol. 440 (2013), p. 18–22
We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maizechlorotic mottle virus (MCMV). [...]

Non-labelled surface sensitive techniques as platforms for pharmaceutical nanotechnology research

Publication year: 2013
Authors: Huamin Liang, 2013
Published in: Doctor's Dissertation (article-based)
Insufficient delivery of drugs to the target sites like tumors and cells has been a barrier for achieving satisfying therapeutic effects in many diseases. Distribution and exposure of drugs to normal and healthy tissues may enhance the possibility of side effects and toxicity in vivo. Nanoparticle (NP) drug delivery systems have been developed to enable targeting of drugs to target sites and at the same time also reduce or even eliminate the distribution and exposure of drugs to non-targeted sites (normal and healthy tissues). The interactions of ligand attached NPs with specific receptors on the cell surface enable intracellular delivery of drugs. [...]

Specific water uptake of thin films from nanofibrillar cellulose

Publication year: 2013
Authors: Katri S. Kontturi (a), Eero Kontturi (a), Janne Laine (a)
Published in: J.Mater. Chem. A, 2013
Swelling of NFC films was surveyed by quartz crystal microbalance with dissipation monitoring coupled with H2O/D2O exchange and surface plasmon resonance. The influence of charged groups on swelling was investigated by neutralizing the charge of NFC with cationic polyelectrolytes or increasing the charge with carboxymethyl cellulose (CMC). [...]

Elucidating the Signal Responses of Multi-Parametric Surface Plasmon Resonance Living Cell Sensing: A Comparison between Optical Modeling and Drug–MDCKII Cell Interaction Measurements

Publication year: 2013
Authors: Tapani Viitala 1, Niko Granqvist 1, Susanna Hallila 1, Marjo Yliperttula 1, Manuela Raviña 2
Published in: PLoS ONE, 2013, Vol. 8(8), e72192
In vitro cell-based assays are widely used during the drug discovery and development process to test the biological activity of new drugs. Most of the commonly used cell-based assays, however, lack the ability to measure in real-time or under dynamic conditions (e.g. constant flow). In this study a multi-parameter surface plasmon resonance approach in combination with living cell sensing has been utilized for monitoring drug-cell interactions in real-time, under constant flow and without labels. [...]