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Publications

Peer-reviewed publications

Different Oligomeric States of the Tumor Suppressor p53 Show Identical Binding Behavior Towards the S100beta Homodimer

Publication year: 2022
Authors: Wei A.A.J. a, Iacobucci C. a*, Schultze W. a, Ihling C.H. a, Arlt C.a*, Sinz A. a*
Published in: ChemBioChem, e202100665

The tumor suppressor protein p53 is a transcription factor that is referred to as the “guardian of the genome” and plays an important role in cancer development. P53 is active as a homotetramer; the S100β homodimer binds to the intrinsically disordered C-terminus of p53 affecting its transcriptional activity. The p53/S100β complex is regarded as highly…


Peer-reviewed publications

Accurate Correction of the “Bulk Response” in Surface Plasmon Resonance Sensing Provides New Insights on Interactions Involving Lysozyme and Poly(ethylene glycol)

Publication year: 2022
Authors: Svirelis J. a, Andersson J. a, Stradner A. b, Dahlin A. a*
Published in: ACS Sensors 2022

Surface plasmon resonance is a very well-established surface sensitive technique for label-free analysis of biomolecular interactions, generating thousands of publications each year. An inconvenient effect that complicates interpretation of SPR results is the “bulk response” from molecules in solution, which generate signals without really binding to the surface. Here we present a physical model for…


Peer-reviewed publications

Biofunctionalization of Graphene-Based FET Sensors through Heterobifunctional Nanoscaffolds: Technology Validation toward Rapid COVID-19 Diagnostics and Monitoring

Publication year: 2022
Authors: Piccinini E. *, Fenoy G.E. , Cantillo A.L. , Allegretto J.A. , Scotto J. , Piccinini J.M. , Marmisollé W.A. , Azzaroni O. *
Published in: Advanced Materials Interfaces, 2102526

The biofunctionalization of graphene field-effect transistors (GFETs) through vinylsulfonated-polyethyleneimine nanoscaffold is presented for enhanced biosensing of severe acute respiratory-related coronavirus 2 (SARS-CoV-2) spike protein and human ferritin, two targets of great importance for the rapid diagnostic and monitoring of individuals with COVID-19. The heterobifunctional nanoscaffold enables covalent immobilization of binding proteins and antifouling polymers while…


Peer-reviewed publications

Computer-Aided Engineering of Staphylokinase Toward Enhanced Affinity and Selectivity for Plasmin

Publication year: 2022
Authors: Nikitin D. a,b,1, Mican J. a,b,d,1, Toul M. a,b,1, Bednar D. a,b, Peskova M. c , Kittova P. c , Thalerova S. a,c, Vitecek J. a,c, Damborsky J. a,b, Mikulik R. a,d, Fleishman S.J. e , Prokop Z. a,b,*, Marek M. a,b,*
Published in: Computational and Structural Biotechnology Journal (2022)

Cardio- and cerebrovascular diseases are leading causes of death and disability, resulting in one of the highest socio-economic burdens of any disease type. The discovery of bacterial and human plasminogen activators and their use as thrombolytic drugs have revolutionized treatment of these pathologies. Fibrin-specific agents have an advantage over non-specific factors because of lower rates…


Peer-reviewed publications

Impact of polysaccharide and protein interactions on membrane fouling: Particle deposition and layer formation

Publication year: 2022
Authors: Zhang C. a, Bao Q. a, Wu H. a, Shao M. a, Wang X. a, Xu Q. a*
Published in: Chemosphere, 296, 134056

Membrane fouling, which limits the application of membrane bioreactors, has received considerable research attention in recent years. In this work, filtration modeling was performed in combination with surface plasmon resonance (SPR) analysis to investigate the membrane fouling mechanism. Sodium alginate (SA) and bovine serum albumin (BSA) were used to perform dead-end filtration on hydrophilic and…


Peer-reviewed publications

Rapid and sensitive detection of PD-L1 exosomes using Cu-TCPP 2D MOF as a SPR sensitizer

Publication year: 2022
Authors: Wang Y. a, Mao Z. b, Chen Q. c, Koh K. d, Hu X. a **, Chen H. a *
Published in: Biosensors and Bioelectronics, 201, 113954

Two-dimensional metal organic framework (2D MOF Cu-TCPP) with significantly enhanced photoelectric properties was synthesized by a simple hydrothermal method. The π-stacked electroactive porphyrin molecules of TCPP-based 2D MOF carry out charge transport in the MOF structure. The d-d band transition of Cu2+ and its 2D ultra-thin characteristics can produce excellent near-infrared light absorption to couple with SPR. Three key parameters including the refractive index sensitivity, detection…


Peer-reviewed publications

Aptamer-Assisted Protein Orientation on Silver Magnetic Nanoparticles: Application to Sensitive Leukocyte Cell-Derived Chemotaxin 2 Surface Plasmon Resonance Sensors

Publication year: 2022
Authors: Zhu H. a, Lu Y. a, Xia J. a , Liu Y. cd, Chen J. ac, Lee J. e, Koh K. f, Chen H. ab*
Published in: Analytical Chemistry, 94, 2109-2118

Leukocyte cell-derived chemotaxin 2 (LECT2) has been proved to be a potential biomarker for the diagnosis of liver fibrosis. In this work, a sensitive surface plasmon resonance (SPR) assay for LECT2 analysis was developed. Tyrosine kinase with immune globulin-like and epidermal growth factor-like domains 1 (Tie1) is an orphan receptor of LECT2 with a C-terminal…


Peer-reviewed publications

Novel diagnostic and prognostic factors for the advanced melanoma based on the glycosylation-related changes studied by biophysical profiling methods

Publication year: 2022
Authors: Sobiepanek A. a * .Kowalska P. D ab, Szota M. c , Grzywa T.M. def ., Nowak J g, Włodarski P.K. d, Galus R. h, Jachimska B. c, Kobiela T. a**
Published in: Biosensors and Bioelectronics, 203, 114046

Melanoma is a life-threatening disease due to the early onset of metastasis and frequent resistance to the applied treatment. For now, no single histological, immunohistochemical or serological biomarker was able to provide a precise predictive value for the aggressive behavior in melanoma patients. Thus, the search for quantifying methods allowing a simultaneous diagnosis and prognosis…


Peer-reviewed publications

Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance

Publication year: 2022
Authors: Norling K. a, Sjöberg M. a, Bally M b., Zhdanov V.P. a,c , Parveen N.* a, Höök F* a.
Published in: Langmuir 2022 38 (8), 2550-2560

The mechanical properties of biological nanoparticles play a crucial role in their interaction with the cellular membrane, in particular for cellular uptake. This has significant implications for the design of pharmaceutical carrier particles. In this context, liposomes have become increasingly popular, among other reasons due to their customizability and easily varied physicochemical properties. With currently…


Peer-reviewed publications

Combining Chemical Functionalization and FinFET Geometry for Field Effect Sensors as Accessible Technology to Optimize pH Sensing

Publication year: 2021
Authors: D.Rani 1, S.Rollo 1,2, W.Olthuis 2, S.Krishnamoorthy 1, C.P.García 1
Published in: Chemosensors 2021, Vol. 9(2), 20

Field Effect Transistors (FETs) have led the progress of applications measuring the acidity in aqueous solutions thanks to their accuracy, ease of miniaturization and capacity of multiplexing. The signal-to-noise ratio and linearity of the sensors are two of the most relevant figures of merit that can facilitate the improvements of these devices. In this work…