Publications

It is a challenge to interpret the signals obtained by Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) accompanied with in the significant changes of the density and/or refractivity of media. [...]

In this paper, we report a new biosensor for detection of the cardiac biomarker Creatine Kinase (CK-MB) using Surface Plasmon Surface (SPR) technique, with the purpose of the diagnosis of Acute Myocardial Infarction (AMI). [...]

Surface plasmon resonance is a label-free biophysical technique that is widely used in investigating biomolecular interactions, including protein-protein, protein-DNA, and protein-small molecule binding. [...]

Optical brightening agents (OBAs) are commonly used in textile and paper industry to adjust product brightness and color appearence. Continuous production processes lead to short residence time of the dyes in the fiber suspension, [...]

The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. [...]

In this study, the physicochemical characterization of lysozyme adsorbed on gold was investigated. Through the use of MP-SPR it was possible to establish that the orientation of molecules changes from side-on to between or end-on with increasing surface coverage.[...]

Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) [...]

A Kretschmann-based surface plasmon resonance (SPR) sensing method was used to determine the refractive index and thickness of graphene thin films. The measured complex refractive index (n,k) values of graphene on Au at 670 nm and 785 nm wavelength are 2.85, 0.74 and 3.1, 1.19 respectively. In further analysis, the analysis of sensing performance of graphene on Au SPR sensor for ethanol detection was obtained using Finite Difference Time Domain (FDTD) method.[...]

Hsp70 is a conserved molecular chaperone. How Hsp70 exhibits specialized functions across species remains to be understood. Plasmodium falciparum Hsp70-1 (PfHsp70-1) and Escherichia coli DnaK are cytosol localized molecular chaperones that are important for the survival of these two organisms. [...]

We report a straightforward route for the preparation of flexible, electrochemically stable and easily functionalizable poly(3,4-ethylenedioxythiophene) (PEDOT) composite films deposited on PET foils as biosensing platforms. For this purpose, poly(allylamine) hydrochloride (PAH) was blended with PEDOT to provide amine-bearing sites for further biofunctionalization as well as to improve the mechanical properties of the films.[...]

Thebaine 6-O-demethylase (T6ODM) is an Fe(II)/2-oxoglutarate-dependent dioxygenase catalysing two oxidative O-demethylation reactions in morphine biosynthesis. Its crystal structure revealed a large active site pocket which is at least two times larger than necessary to accommodate a substrate (thebaine or oripavine) molecule.[...]

Polymer brushes not only represent emerging surface platforms for numerous bioanalytical and biological applications but also create advanced surface-tethered systems to mimic real-life biological processes. In particular, zwitterionic and nonionic polymer brushes have been intensively studied because of their extraordinary resistance to nonspecific adsorption of biomolecules (antifouling characteristics) as well as the ability to be functionalized with bioactive molecules.[...]

Real-time label-free techniques are used to profile G protein-coupled receptor (GPCR) signaling pathways in living cells. However, interpreting the label-free signal responses is challenging, [...]

Ultra‐low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra‐low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied.[...]

Coupling of surface plasmon resonance (SPR) detection to asymmetric flow field-flow fractionation (AF4) offers the possibility to study active fractions of bio-separations on real samples, such as [...]

We present a polyelectrolyte brush electrode with high-capacity for protein immobilization that captures and releases proteins by an electrochemical potential. Central to our concept is the use of polyelectrolyte brushes that switch reversibly by electrochemistry.[...]

The capabilities of biosensors for fast, economic, and user-friendly analysis of complex samples has led to the exploitation of analytical devices for detection, quantification, and monitoring of specific chemical species for various applications. For a sufficiently high surface reactivity toward the adopted bioreceptors, a thin functional layer is required to enable coupling of the target biomolecules and to provide good stability in the presence of a sample matrix.[...]

Multiple myeloma (MM) is a neoplasm of plasma cells that secrete patient specific monoclonal immunoglobulins. A recognized problem in MM treatment is the early recognition of minimal residual disease (MRD), the major cause of relapse. [...]

Enzyme immobilization is extensively studied to improve enzyme properties in catalysis and analytical applications. Here, we introduce a simple and versatile enzyme immobilization platform based on adhesion‐promoting peptides, namely Matter‐tags. Matter‐tags immobilize enzymes in an oriented way as a dense monolayer.[...]

Thiolated self-assembled monolayers (SAMs) are typically used to anchor on a gold surface biomolecules serving as recognition elements for biosensor applications. Here, the design and synthesis of N-(2-hydroxyethyl)-3-mercaptopropanamide (NMPA) in biotinylated mixed SAMs is proposed as an alternative strategy with respect to on-site multistep functionalization of SAMs prepared from solutions of commercially available thiols.[...]