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Application Notes

AN#170 Profiling of G protein-coupled receptor (GPCR) stimulation by small compounds in live cells

G protein-coupled receptors (GPCRs) are a large family of transmembrane  proteins which are involved in many physiological and pathological processes. Extracellular stimuli result in cellular responses, which lead to ordered and dynamic redistribution of intracellular contents. Cellular responses are receptor-type specific. Accordingly, GPCRs are divided into four subclasses, Gi/o, Gq/11, Gs, and G12/13. Real-time label-free…

Application Notes

AN#169 Diagnostic method for serological testing of Covid-19 antibodies

AN#169 Diagnostic method for serological testing of Covid-19 antibodies

Considering the indisputable need for Covid-19 related diagnostic tools, we have developed a dedicated serological testing method based on MP-SPR technology. The combination of MP-SPR instrument and optimized sensor format functionalized with virus proteins enabled rapid detection of SARS-CoV-2 antigen-specific antibodies in human serum. The measurement accuracy has been proven with seropositive patient samples and validated with certified reference serum materials. As alternative…

Application Notes

AN#168 Regenerable avidin kit assays Part II: Biomolecule affinity and concentration studies


MP-SPR Navi™ Regenerable avidin kit is an excellent tool in development of MP-SPR based assays. Here, the specific binding affinity of lipid based molecules towards sensor-captured protein was analyzed. Also, concentration analysis of diagnostically relevant biomarkers were performed in human serum samples. Utilizing Regenerable avidin kit reduces time in assay development since there is no…

Peer-reviewed publications

Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays

Publication year: 2020
Authors: Solin K. 1, Beaumont M. 1,2, Rosenfeldt S. 3, Orelma H. 4, Borghei M. 1, Bacher M. 2, Opietnik M. 5, Rojas O. J. 1,6
Published in: Small, 2020, Vol. 16, Issue 50, 2004702

Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between…

Peer-reviewed publications

Novel personalized cancer vaccine platform based on Bacillus Calmette-Guèrin

Publication year: 2021
Authors: Ylösmäki E. # 1 2, Fusciello M. # 1 2, Martins B. # 1 2, Feola S. # 1 2, Hamdan F. 1 2, Chiaro J. 1 2, Ylösmäki L. 1 2 3, Vaughan M. 3, Viitala T. 4, Kulkarni P. 5, Cerullo V. 6 2 7 8
Published in: Journal for ImmunoTherapy of Cancer, 2021, Vol. 9, p. 002707

Background: Intratumoral BCG therapy, one of the earliest immunotherapies, can lead to infiltration of immune cells into a treated tumor. However, an increase in the number of BCG-induced tumor-specific T cells in the tumor microenvironment could lead to enhanced therapeutic effects. Methods: Here, we have developed a novel cancer vaccine platform based on BCG that…

Peer-reviewed publications

A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccines

Publication year: 2022
Authors: Feola S. 1 2 3, Chiaro J. # 1 2 3, Martins B. # 1 2 3, Russo S. 1 2 3, Fusciello M. 1 2 3, Ylösmäki E. 1 2 3, Bonini C. 4, Ruggiero E. 4, Hamdan F. 1 2 3, Feodoroff M. 1 2 3 5 6, Antignani G. 1 2 3, Viitala T. 7, Pesonen S. 8, Grönholm M. 1 2 3 5, Branca R. 9, Lehtiö J. 9, Cerullo V. 1 2 3 5 9 10
Published in: eLife, 2022, Vol. 11, p. 71156

Besides the isolation and identification of major histocompatibility complex I-restricted peptides from the surface of cancer cells, one of the challenges is eliciting an effective antitumor CD8+ T-cell-mediated response as part of therapeutic cancer vaccine. Therefore, the establishment of a solid pipeline for the downstream selection of clinically relevant peptides and the subsequent creation of…

Peer-reviewed publications

Protein A/G-based surface plasmon resonance biosensor for regenerable antibody-mediated capture and analysis of nanoparticles

Publication year: 2022
Authors: Parkkila P. 1 2 3, Härkönen K. 4, Ilvonen P. 4, Laitinen S. 4, Viitala T. 1 2
Published in: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 654 p.130015

Characterization of nanoparticles (NPs) and their subpopulations in heterogeneous samples is of utmost importance, for example, during the initial design of targeted NP therapies and the different phases of their production cycle. Biological NPs such as extracellular vesicles (EVs) have shown promise in improving the drug delivery capabilities compared to traditional NP-based therapies, for example, in treating…

Peer-reviewed publications

A novel cancer vaccine for melanoma based on an approved vaccine against measles, mumps, and rubella

Publication year: 2022
Authors: Fusciello M. 1 2 3, Ylösmäki E. 1 2 3, Feola S. 1 2 3, Uoti A. 1 2 3, Martins B. 1 2 3, Aalto K. 4, Hamdan F. 1 2 3, Chiaro J. 1 2 3, Russo S. 1 2 3, Viitala T. 4, Cerullo V. 1 2 3 5
Published in: Molecular Therapy Oncolytics, 2022, Vol. 25, p. 137-145

Common vaccines for infectious diseases have been repurposed as cancer immunotherapies. The intratumoral administration of these repurposed vaccines can induce immune cell infiltration into the treated tumor. Here, we have used an approved trivalent live attenuated measles, mumps, and rubella (MMR) vaccine in our previously developed PeptiENV cancer vaccine platform. The intratumoral administration of this…

Peer-reviewed publications

SPR-Based Detection of ASF Virus in Cells

Publication year: 2022
Authors: Capo A. 1 2, Calabrese A. 1 2, Frant M. 3, Walczak M. 3, Szczotka-Bochniarz A. 3, Manessis G. 4, Bossis I. 5, Staiano M. 1, D'Auria S. 6, Varriale A. 1 2
Published in: International Journal of Molecular Sciences, 2022, Vol 23, p. 7463

African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on…

Peer-reviewed publications

Development of a Syrian hamster anti-PD-L1 monoclonal antibody enables oncolytic adenoviral immunotherapy modelling in an immunocompetent virus replication permissive setting

Publication year: 2023
Authors: Clubb J. 1,2,3, Kudling T. 1,3, Girych M. 4, Haybout L. 1,3, Pakola S. 1,3, Hamdan F. 5,6, Cervera-Carrascon V. 1,2,3, Hemmes A. 7, Grönberg-Vähä-Koskela S. 1,3,8, Santos S. 1,2,3, Quixabeira D. 1,2,3, Basnet S. 1,3, Heiniö C. 1,3, Arias V. 1,3, Jirovec E. 1,3, Kaptan S. 4, Havunen R. 1,2,3, Sorsa S. 1,2,3, Erikat A. 9, Schwartz J. 10, Anttila M. 11, Aro K. 8,12, Viitala T. 6, Vattulainen I. 4, Cerullo V. 5,6, Kanerva A. 13, Hemminki A. 1,2,3,8
Published in: Frontiers in Immunology, 2023, Vol. 14 p. 1060540

Introduction: Immune checkpoint inhibitors (ICIs) have revolutionized the treatment of cancer, but preclinical testing of hypotheses such as combination therapies has been complicated, in part due to species incompatibility issues. For example, one of few known permissive animal models for oncolytic adenoviruses is the Syrian hamster, for which an ICI, mainly an anti-PD-L1 monoclonal antibody (mAb)…