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In-situ and real-time probing cellulase biosensor formation and its interaction with lignosulfonate in varied media

Publication year: 2021
Authors: Peipei Wang a,1, Tian Liu a,1, Yena Liu a,1, Jing Tian a, Xinyu Zhang a, Jiaqi Guo a, Yongcan Jin a, Huining Xiao b, Junlong Song a
Published in: Sensors and Actuators B: Chemical, Vol. 329 (2021), 129114
It is a challenge to interpret the signals obtained by Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) accompanied with in the significant changes of the density and/or refractivity of media. [...]

Development of a novel biosensor for Creatine Kinase (CK-MB) using Surface Plasmon Resonance (SPR)

Publication year: 2021
Authors: André L.Ferreira a,1, Lucas Lima b,1, Ariana S.Moraes a,1, Rafael J.G.Rubira c,1, Carlos J.L.Constantino c, Fabio L.Leite a, Adriana O.Delgado-Silva a, Marystela Ferreira a
Published in: Applied Surface Science, Vol. 554 (2021), 149565
In this paper, we report a new biosensor for detection of the cardiac biomarker Creatine Kinase (CK-MB) using Surface Plasmon Surface (SPR) technique, with the purpose of the diagnosis of Acute Myocardial Infarction (AMI). [...]

SPRD: a surface plasmon resonance database of common factors for better experimental planning

Publication year: 2021
Authors: P.B. Tiwari 1, C. Bencheqroun 2, M. Lemus 1, T. Shaw 1, M. Kouassi-Brou 1,3, A. Alaoui 2, A. Üren 1
Published in: BMC Molecular and Cell Biology, Vol. 22, 17 (2021)
Surface plasmon resonance is a label-free biophysical technique that is widely used in investigating biomolecular interactions, including protein-protein, protein-DNA, and protein-small molecule binding. [...]

Real-time adsorption of optical brightening agents on cellulose thin films

Publication year: 2021
Authors: C. Sampl a,b, S.Eyley c, W. Thielemans c, U.Hirn a,b,1, S.Spirk a,b
Published in: Carbohydrate Polymers, Vol. 261 (2021), 117826
Optical brightening agents (OBAs) are commonly used in textile and paper industry to adjust product brightness and color appearence. Continuous production processes lead to short residence time of the dyes in the fiber suspension, [...]

Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

Publication year: 2021
Authors: Xue Zhang a, Tapani Viitala b,1, Riina Harjumäki a,b, Alma Kartal-Hodzic b, Juan José Valle-Delgado a, Monika Österberg a
Published in: Journal of Colloid and Interface Science, Vol. 584 (2021), p. 310-319
The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. [...]

Mechanism of lysozyme adsorption onto gold surface determined by quartz crystal microbalance and surface plasmon resonance

Publication year: 2020
Authors: Komorek P., Wałek M., Jachimska B.
Published in: Bioelectrochemistry, 2020, Vol. 135, p. 107582
In this study, the physicochemical characterization of lysozyme adsorbed on gold was investigated. Through the use of MP-SPR it was possible to establish that the orientation of molecules changes from side-on to between or end-on with increasing surface coverage.[...]

Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone

Publication year: 2020
Authors: Riikka Peltomaa a,b,1, Zdeněk Farka a,c, Matthias J. Mickert a,2, Julian C. Brandmeier a, Matěj Pastucha c, Antonín Hlaváček d, Mónica Martínez-Orts e, Ángeles Canales e, Petr Skládal c, Elena Benito-Peña b, María C. Moreno-Bondi b, Hans H. Gorris a
Published in: Biosensors and Bioelectronics, Vol. 170, p. 112683
Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) [...]

CVD-grown Graphene-on-Au characterization and sensing using Kretschmann-based SPR

Publication year: 2020
Authors: Jamil N.A.1, Mei G.S.1, Zain A.R. 1,2, Hewak D. 3, Huang C-C. 3, Mohamed M.A. 1, Majlis B.Y. 1, Menon P.S.1,*
Published in: Electron Devices Technology and Manufacturing Conference (EDTM), IEEE
A Kretschmann-based surface plasmon resonance (SPR) sensing method was used to determine the refractive index and thickness of graphene thin films. The measured complex refractive index (n,k) values of graphene on Au at 670 nm and 785 nm wavelength are 2.85, 0.74 and 3.1, 1.19 respectively. In further analysis, the analysis of sensing performance of graphene on Au SPR sensor for ethanol detection was obtained using Finite Difference Time Domain (FDTD) method.[...]

Comparative Characterization of Plasmodium falciparum Hsp70-1 Relative to E. coli DnaK Reveals the Functional Specificity of the Parasite Chaperone

Publication year: 2020
Authors: Lebepe C. 1, Matambanadzo P. 1, Makhoba X. 2, Achilonu I. 3, Zininga T. 1,4, Shonhai A. 1,*
Published in: Biomolecules 2020, Vol. 10(6), p. 856
Hsp70 is a conserved molecular chaperone. How Hsp70 exhibits specialized functions across species remains to be understood. Plasmodium falciparum Hsp70-1 (PfHsp70-1) and Escherichia coli DnaK are cytosol localized molecular chaperones that are important for the survival of these two organisms. [...]

PEDOT-polyamine composite films for bioelectrochemical platforms -flexible and easy to derivatize

Publication year: 2020
Authors: Sappia L. +a, Piccinini E. +a, von Binderling C. a, Knoll W. bcd, Marmisollé W. *a, Azzorini O. *acd
Published in: Material Sciece and Engineering: C, 2020, Vol. 109, p.110575
We report a straightforward route for the preparation of flexible, electrochemically stable and easily functionalizable poly(3,4-ethylenedioxythiophene) (PEDOT) composite films deposited on PET foils as biosensing platforms. For this purpose, poly(allylamine) hydrochloride (PAH) was blended with PEDOT to provide amine-bearing sites for further biofunctionalization as well as to improve the mechanical properties of the films.[...]

It takes two to tango - The case of thebaine 6-O-demethylase

Publication year: 2020
Authors: Kachhap S. a*, Wojdyla Z. a*, Komorek P. a, Kluza A. a, Kurpiewska K. ab, Jachimska B. a,Borowski T.a
Published in: nternational Journal of Biological Macromolecules, 2020, Vol. 163, p. 718-729
Thebaine 6-O-demethylase (T6ODM) is an Fe(II)/2-oxoglutarate-dependent dioxygenase catalysing two oxidative O-demethylation reactions in morphine biosynthesis. Its crystal structure revealed a large active site pocket which is at least two times larger than necessary to accommodate a substrate (thebaine or oripavine) molecule.[...]

Surface Preconditioning Influences the Antifouling Capabilities of Zwitterionic and Nonionic Polymer Brushes

Publication year: 2020
Authors: Víšová I. 1, Vrabcová M. 1, Forinová M. 1, Zhigunová Y. 1, Mironov V. 1, Houska . 1, Bittrich E. 2, Eichhorn K. 2, Hashim H. 3,4, Schovánek P. 1,5, Dejneka A. 1, Vaisocherová - Lísalová H. 1
Published in: Langmuir, 2020, Vol. 36, 29, 8485–8493
Polymer brushes not only represent emerging surface platforms for numerous bioanalytical and biological applications but also create advanced surface-tethered systems to mimic real-life biological processes. In particular, zwitterionic and nonionic polymer brushes have been intensively studied because of their extraordinary resistance to nonspecific adsorption of biomolecules (antifouling characteristics) as well as the ability to be functionalized with bioactive molecules.[...]

Label-Free Analysis with Multiple Parameters Separates G Protein-Coupled Receptor Signaling Pathways

Publication year: 2020
Authors: Temu Suutari*, Sabrina N. Rahman, Henry F. Vischer, Dick van Iperen, Arto Merivaara, Marjo Yliperttula, Rob Leurs, Jeroen Kool, Tapani Viitala*
Published in: Analytical Chemistry, 2020, Vol. 92, 21, p. 14509–14516
Real-time label-free techniques are used to profile G protein-coupled receptor (GPCR) signaling pathways in living cells. However, interpreting the label-free signal responses is challenging, [...]

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

Publication year: 2020
Authors: Víšová I., Smolková B., Uzhytchak M., Vrabcová M., Zhigunova Y., Houska M. ,Surman F., de los Santos Pereira A.,Lunov O., Dejneka A., Vaisocherová‐Lísalová H.
Published in: Macromolecular Bioscience, 2020, Vol. 20, 3, p. 1900351
Ultra‐low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra‐low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied.[...]

Asymmetric flow field-flow fractionation coupled to surface plasmon resonance detection for analysis of therapeutic proteins in blood serum

Publication year: 2020
Authors: Mats Leeman 1, Willem M. Albers 2, Radoslaw Bombera 2, Johana Kuncova-Kallio 2, Jussipekka Tuppurainen 2, Lars Nilsson 3
Published in: Analytical and Bioanalytical Chemistry, 2020
Coupling of surface plasmon resonance (SPR) detection to asymmetric flow field-flow fractionation (AF4) offers the possibility to study active fractions of bio-separations on real samples, such as [...]

Generic High-Capacity Electrochemical Capture and Release of Proteins by Polyelectrolyte Brushes

Publication year: 2020
Authors: Ferrand-Drake del Castillo G., Adali Z., L. N. Hailes R., Xiong K., Dahlin A.*
Published in: Preprint in Chem RXIV, 2020
We present a polyelectrolyte brush electrode with high-capacity for protein immobilization that captures and releases proteins by an electrochemical potential. Central to our concept is the use of polyelectrolyte brushes that switch reversibly by electrochemistry.[...]

Functional Plasma Polymerized Surfaces for Biosensing

Publication year: 2020
Authors: Makhneva* E., Barillas L 1., Farka Z. 2, Pastucha M. 2, Skládal P. 2, Weltmann K.D. 1, Fricke K. 1
Published in: Applied Material Interfaces, 2020, Vol.12, 14, p.17100–17112
The capabilities of biosensors for fast, economic, and user-friendly analysis of complex samples has led to the exploitation of analytical devices for detection, quantification, and monitoring of specific chemical species for various applications. For a sufficiently high surface reactivity toward the adopted bioreceptors, a thin functional layer is required to enable coupling of the target biomolecules and to provide good stability in the presence of a sample matrix.[...]

Personalized immunoglobulin aptamers for detection of multiple myeloma minimal residual disease in serum

Publication year: 2020
Authors: Claudia Tapia-Alveal, Timothy R. Olsen, Tilla S. Worgall
Published in: Communications Biology, Vol. 3 (2020), 781
Multiple myeloma (MM) is a neoplasm of plasma cells that secrete patient specific monoclonal immunoglobulins. A recognized problem in MM treatment is the early recognition of minimal residual disease (MRD), the major cause of relapse. [...]

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Publication year: 2020
Authors: Dedisch S. 12, Wiens A. 2, Davari M.D. 2, Söder D. 1, Rodriguez‐Emmenegger C. 13, Jakob F. 12, Schwaneberg U.*12
Published in: Biotechnology and Bioengineering, 2020, Vol. 117, Issue1, p. 49-61
Enzyme immobilization is extensively studied to improve enzyme properties in catalysis and analytical applications. Here, we introduce a simple and versatile enzyme immobilization platform based on adhesion‐promoting peptides, namely Matter‐tags. Matter‐tags immobilize enzymes in an oriented way as a dense monolayer.[...]

Enhancing the Sensitivity of Biotinylated Surfaces by Tailoring the Design of the Mixed Self-Assembled Monolayer Synthesis

Publication year: 2020
Authors: Blasi D. ‡†, Sarcina L. ‡§, Tricase A. ‡§, Stefanachi A. ‖, Leonetti F.*‖, Alberga D. $, Mangiatordi* Ͱ, Manoli K. †§, Scamarcio G. ◊∫, Picca R.A. *†§, Torsi L. †§#
Published in: ACS Omega 2020, Vol. 5, 27, p. 16762–16771
Thiolated self-assembled monolayers (SAMs) are typically used to anchor on a gold surface biomolecules serving as recognition elements for biosensor applications. Here, the design and synthesis of N-(2-hydroxyethyl)-3-mercaptopropanamide (NMPA) in biotinylated mixed SAMs is proposed as an alternative strategy with respect to on-site multistep functionalization of SAMs prepared from solutions of commercially available thiols.[...]