You can also use advanced search to find preferred publications.
AN#170 Profiling of G protein-coupled receptor (GPCR) stimulation by small compounds in live cells
G protein-coupled receptors (GPCRs) are a large family of transmembrane proteins which are involved in many physiological and pathological processes. Extracellular stimuli result in cellular responses, which lead to ordered and dynamic redistribution of intracellular contents. Cellular responses are receptor-type specific. Accordingly, GPCRs are divided into four subclasses, Gi/o, Gq/11, Gs, and G12/13. Real-time label-free…
AN#169 Diagnostic method for serological testing of Covid-19 antibodies
Considering the indisputable need for Covid-19 related diagnostic tools, we have developed a dedicated serological testing method based on MP-SPR technology. The combination of MP-SPR instrument and optimized sensor format functionalized with virus proteins enabled rapid detection of SARS-CoV-2 antigen-specific antibodies in human serum. The measurement accuracy has been proven with seropositive patient samples and validated with certified reference serum materials. As alternative…
AN#168 Regenerable avidin kit assays Part II: Biomolecule affinity and concentration studies
MP-SPR Navi™ Regenerable avidin kit is an excellent tool in development of MP-SPR based assays. Here, the specific binding affinity of lipid based molecules towards sensor-captured protein was analyzed. Also, concentration analysis of diagnostically relevant biomarkers were performed in human serum samples. Utilizing Regenerable avidin kit reduces time in assay development since there is no…
Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays
Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between…
Novel personalized cancer vaccine platform based on Bacillus Calmette-Guèrin
Background: Intratumoral BCG therapy, one of the earliest immunotherapies, can lead to infiltration of immune cells into a treated tumor. However, an increase in the number of BCG-induced tumor-specific T cells in the tumor microenvironment could lead to enhanced therapeutic effects. Methods: Here, we have developed a novel cancer vaccine platform based on BCG that…
A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccines
Besides the isolation and identification of major histocompatibility complex I-restricted peptides from the surface of cancer cells, one of the challenges is eliciting an effective antitumor CD8+ T-cell-mediated response as part of therapeutic cancer vaccine. Therefore, the establishment of a solid pipeline for the downstream selection of clinically relevant peptides and the subsequent creation of…
Protein A/G-based surface plasmon resonance biosensor for regenerable antibody-mediated capture and analysis of nanoparticles
Characterization of nanoparticles (NPs) and their subpopulations in heterogeneous samples is of utmost importance, for example, during the initial design of targeted NP therapies and the different phases of their production cycle. Biological NPs such as extracellular vesicles (EVs) have shown promise in improving the drug delivery capabilities compared to traditional NP-based therapies, for example, in treating…
A novel cancer vaccine for melanoma based on an approved vaccine against measles, mumps, and rubella
Common vaccines for infectious diseases have been repurposed as cancer immunotherapies. The intratumoral administration of these repurposed vaccines can induce immune cell infiltration into the treated tumor. Here, we have used an approved trivalent live attenuated measles, mumps, and rubella (MMR) vaccine in our previously developed PeptiENV cancer vaccine platform. The intratumoral administration of this…
SPR-Based Detection of ASF Virus in Cells
African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on…
Development of a Syrian hamster anti-PD-L1 monoclonal antibody enables oncolytic adenoviral immunotherapy modelling in an immunocompetent virus replication permissive setting
Introduction: Immune checkpoint inhibitors (ICIs) have revolutionized the treatment of cancer, but preclinical testing of hypotheses such as combination therapies has been complicated, in part due to species incompatibility issues. For example, one of few known permissive animal models for oncolytic adenoviruses is the Syrian hamster, for which an ICI, mainly an anti-PD-L1 monoclonal antibody (mAb)…