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Oxidized xylan additive for nanocellulose films – A swelling modifier

Publication year: 2021
Authors: Palasingh Ch. a, Ström A. a, Amer H. b,c, Nypelö T. a,d
Published in: International Journal of Biological Macromolecules, Vol. 180, p. 753-759
Polymeric wood hemicelluloses are depicted to join cellulose, starch and chitosan as key polysaccharides for sustainable materials engineering. However, the approaches to incorporate hemicelluloses in [...]

In-situ and real-time probing cellulase biosensor formation and its interaction with lignosulfonate in varied media

Publication year: 2021
Authors: Peipei Wang a,1, Tian Liu a,1, Yena Liu a,1, Jing Tian a, Xinyu Zhang a, Jiaqi Guo a, Yongcan Jin a, Huining Xiao b, Junlong Song a
Published in: Sensors and Actuators B: Chemical, Vol. 329 (2021), 129114
It is a challenge to interpret the signals obtained by Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) accompanied with in the significant changes of the density and/or refractivity of media. [...]

Development of a novel biosensor for Creatine Kinase (CK-MB) using Surface Plasmon Resonance (SPR)

Publication year: 2021
Authors: André L.Ferreira a,1, Lucas Lima b,1, Ariana S.Moraes a,1, Rafael J.G.Rubira c,1, Carlos J.L.Constantino c, Fabio L.Leite a, Adriana O.Delgado-Silva a, Marystela Ferreira a
Published in: Applied Surface Science, Vol. 554 (2021), 149565
In this paper, we report a new biosensor for detection of the cardiac biomarker Creatine Kinase (CK-MB) using Surface Plasmon Surface (SPR) technique, with the purpose of the diagnosis of Acute Myocardial Infarction (AMI). [...]

SPRD: a surface plasmon resonance database of common factors for better experimental planning

Publication year: 2021
Authors: P.B. Tiwari 1, C. Bencheqroun 2, M. Lemus 1, T. Shaw 1, M. Kouassi-Brou 1,3, A. Alaoui 2, A. Üren 1
Published in: BMC Molecular and Cell Biology, Vol. 22, 17 (2021)
Surface plasmon resonance is a label-free biophysical technique that is widely used in investigating biomolecular interactions, including protein-protein, protein-DNA, and protein-small molecule binding. [...]

Real-time adsorption of optical brightening agents on cellulose thin films

Publication year: 2021
Authors: C. Sampl a,b, S.Eyley c, W. Thielemans c, U.Hirn a,b,1, S.Spirk a,b
Published in: Carbohydrate Polymers, Vol. 261 (2021), 117826
Optical brightening agents (OBAs) are commonly used in textile and paper industry to adjust product brightness and color appearence. Continuous production processes lead to short residence time of the dyes in the fiber suspension, [...]

Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

Publication year: 2021
Authors: Xue Zhang a, Tapani Viitala b,1, Riina Harjumäki a,b, Alma Kartal-Hodzic b, Juan José Valle-Delgado a, Monika Österberg a
Published in: Journal of Colloid and Interface Science, Vol. 584 (2021), p. 310-319
The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. [...]

Terminal deoxynucleotidyl transferase-mediated formation of protein binding polynucleotides

Publication year: 2021
Authors: J. Ashley 1,2, A.-L. Schaap-Johansen 1, M. Mohammadniaei 1, M. Naseri 1, P. Marcatili 1, M. Prado 2, Y. Sun 1
Published in: Nucleic Acids Research, 2021, Vol. 49, Issue 2, p. 1065–1074
Terminal deoxynucleotidyl transferase (TdT) enzyme plays an integral part in the V(D)J recombination, allowing for the huge diversity n expression of immunoglobulins and T-cell receptors [...]

Combining Chemical Functionalization and FinFET Geometry for Field Effect Sensors as Accessible Technology to Optimize pH Sensing

Publication year: 2021
Authors: D.Rani 1, S.Rollo 1,2, W.Olthuis 2, S.Krishnamoorthy 1, C.P.García 1
Published in: Chemosensors 2021, Vol. 9(2), 20
Field Effect Transistors (FETs) have led the progress of applications measuring the acidity in aqueous solutions thanks to their accuracy, ease of miniaturization and capacity of multiplexing. [...]

Characterisation of a unique linker segment of the Plasmodium falciparum cytosol localised Hsp110 chaperone

Publication year: 2021
Authors: G. Chakafana a,b, P.T.Mudau a, T.Zininga a,c, A.Shonhai a
Published in: International Journal of Biological Macromolecules, 2021, Vol.180, p. 272-285
Plasmodium falciparum expresses two essential cytosol localised chaperones; PfHsp70-1 and PfHsp70-z. PfHsp70-z (Hsp110 homologue) is thought to facilitate nucleotide exchange function of PfHsp70-1. PfHsp70-1 is a refoldase, [...]

Mutation of GGMP Repeat Segments of Plasmodium falciparum Hsp70-1 Compromises Chaperone Function and Hop Co-Chaperone Binding

Publication year: 2021
Authors: S. Makumire 1,2 , T. Harmfree Dongola 1, G. Chakafana 1,3, L. Tshikonwane 1, C. Tshikani Chauke 1, T. Maharaj 4, T. Zininga 1,4, A. Shonhai 1
Published in: International Journal of Molecular Sciences, 2021, Vol. 22(4), p. 2226
Parasitic organisms especially those of the Apicomplexan phylum, harbour a cytosol localised canonical Hsp70 chaperone. One of the defining features of this protein is the presence of GGMP repeat residues [...]

Membrane interactions of the anuran antimicrobial peptide HSP1-NH2: Different aspects of the association to anionic and zwitterionic biomimetic systems

Publication year: 2021
Authors: I.P.Gomes a, T.L.Santos a, Souza a., L.O.Nunes a, G.A.Cardoso a,b, C.O.Matos c, L.M.F.Costa a, L.M.Lião c, J.M.Resende b, R.M.Verly a
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, 2021, Vol. 1863 (1), p. 183449
Studies have suggested that antimicrobial peptides act by different mechanisms, such as micellisation, self-assembly of nanostructures and pore formation on the membrane surface. This work presents [...]

Adsorption and Conformation Behavior of Lysozyme on a Gold Surface Determined by QCM-D, MP-SPR, and FTIR

Publication year: 2021
Authors: Komorek P. 1, Martin E. 2, Jachimska B. 1
Published in: International Journal of Molecular Sciences, 2021, Vol. 22(3), p.1322
The physicochemical properties of protein layers at the solid–liquid interface are essential in many biological processes. This study aimed to link the structural analysis of adsorbed lysozyme [...]

Design and synthesis of quinoline-pyrimidine inspired hybrids as potential plasmodial inhibitors

Publication year: 2021
Authors: Kayamba F. a. Malimabe T. b,c, Ademola I.K. d, Pooe O.J. e, Kushwaha N.D. a, Mahlalela M. a, van Zyl R.L. b,c, Gordon M. d, Mudau P.T. f, Zininga T. f,g, Shonhai A. g, Nyamori V.O. h, Karpoormath R. a
Published in: European Journal of Medicinal Chemistry, 2021, Vol. 217, p. 113330
Presently, artemisinin-based combination therapy (ACT) is the first-line therapy of Plasmodium falciparum malaria. With the emergence of malaria parasites that are resistant to ACT, alternative [...]

Bacterial surface, biofilm and virulence properties of Listeriamonocytogenes strains isolated from smoked salmon and fish food contact surfaces

Publication year: 2021
Authors: Sudagidan M. a, Ozalp V.C. b, Öztürk O. c, Zafer Yurt M.N, a, Yavuz O. d, Tasbasi B.B. a, Ucak S. e, Mavili Z.S., Coban A. g, Aydin A. h
Published in: Food Bioscience, 2021, Vol. 41, p.101021
Biofilm formation is one of the defense mechanisms of bacteria against disinfectants and antimicrobials. The aim of this study was to determine biofilm-forming L.monocytogenes from fish processing [...]

Rapid and Oriented Immobilization of Laccases on Electrodes via a Methionine-Rich Peptide

Publication year: 2021
Authors: Haiyang Cui a,b, Lingling Zhang a, Dominik Söder b, Xiaomei Tang a, Mehdi D. Davari a, Ulrich Schwaneberg a,b
Published in: ACS Catalysis, 2021, Vol. 11 (4), p. 2445–2453
Different from other laccases, copper efflux oxidase (CueO) from Escherichia coli possesses an additional methionine-rich segment (MetRich), which is generally considered to be detrimental to its oxidase activity. Herein, we reveal that [...]

In situ analysis of liposome hard and soft protein corona structure and composition in a single label-free workflow

Publication year: 2020
Authors: Kari O. *ab, Ndika J. c, Parkkila P. b, Louna A. a, Lajunen T. ǂa, Puustinen A. d, Viitala T. *§b, Alenius H. ce, Urtti A. af
Published in: Nanoscale, 2020, Vol. 12, p. 1728
Methodological constraints have limited our ability to study protein corona formation, slowing nanomedicine development and their successful translation into the clinic. We determined hard and soft corona structural properties along with the corresponding proteomic compositions on liposomes in a label-free workflow: surface plasmon resonance and a custom biosensor for in situ structure determination on liposomes and corona separation, and proteomics using sensitive nanoliquid chromatography tandem mass spectrometry with open-source bioinformatics platforms.[...]

An investigation of the synergistic effect between magnetite nanoparticles and polypyrrole in nanostructured layer‐by‐layer films

Publication year: 2020
Authors: de Lima L. 1,2, Maciel C. 3, Ferreira A. 1, Rubira R. 4, Constantino C. 4, Ferreira M. *1 1M.
Published in: Journal of Applied Polymer Science, 2020
In this paper, we report the controlled fabrication of layer‐by‐layer (LbL) films deposited on gold substrates with three different supramolecular architectures using polypyrrole (Ppy) and magnetite nanoparticles (Fe3O4‐np), besides conventional poly(allylamine hydrochloride) (PAH) e poly(vinyl sulfonic acid) (PVS) polyelectrolytes, demonstrating the synergistic effect between Ppy and Fe3O4‐np such as a result of their interaction.[...]

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

Publication year: 2020
Authors: Víšová I., Smolková B., Uzhytchak M., Vrabcová M., Zhigunova Y., Houska M. ,Surman F., de los Santos Pereira A.,Lunov O., Dejneka A., Vaisocherová‐Lísalová H.
Published in: Macromolecular Bioscience, 2020, Vol. 20, 3, p. 1900351
Ultra‐low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra‐low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied.[...]

Delineating the Molecular Basis of the Calmodulin–bMunc13-2 Interaction by Cross-Linking/Mass Spectrometry—Evidence for a Novel CaM Binding Motif in bMunc13-2

Publication year: 2020
Authors: Piotrowski C. 1, Moretti R. 2, Ihling C. 1,Haedicke A. 3†, Liepold T. 4, Lipstein N. 5, Meiler J. 2, Jahn O. 4,*, Sinz A. 1,*
Published in: Cells, 2020, Vol.9 (1) , p.136
Exploring the interactions between the Ca2+ binding protein calmodulin (CaM) and its target proteins remains a challenging task. Members of the Munc13 protein family play an essential role in short-term synaptic plasticity, modulated via the interaction with CaM at the presynaptic compartment. [...]

Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor

Publication year: 2020
Authors: Macchia E. 12, Sarcina L. 1, Picca R.A. 1, Manoli K. 1, Di Franco C. 1, Scamarcio G. 34 Torsi L. 125
Published in: Analytical and Bioanalytical Chemistry, 2020, Vol. 412, p. 811–818
Early diagnosis of the infection caused by human immunodeficiency virus type-1 (HIV-1) is vital to achieve efficient therapeutic treatment and limit the disease spreading when the viremia is at its highest level. To this end, a point-of-care HIV-1 detection carried out with label-free, low-cost, and ultra-sensitive screening technologies would be of great relevance.[...]