Peer-reviewed publications

Kinetics of PKCe Activating and Inhibiting Llama Single Chain Antibodies and Their Effect on PKCe Translocation in HeLa Cells

Publication year: 2012

Authors: Milla Summanen 1,2, Niko Granqvist 3, Raimo K. Tuominen 2, Marjo Yliperttula 3, C.Theo Verrips 1, Johannes Boonstra 1; Christophe Blanchetot 1, Elina Ekokoski 2

Published in: PLos ONE, 2012, Vol. 7, Issue 4, e3563

Dysregulation of PKCe is involved in several serious diseases such as cancer, type II diabetes and Alzheimer’s disease.Therefore, specific activators and inhibitors of PKCe hold promise as future therapeutics, in addition to being useful inresearch into PKCe regulated pathways. We have previously described llama single chain antibodies (VHHs) that specificallyactivate (A10, C1 and D1) or…

Peer-reviewed publications

Surface plasmon resonance biosensor with high anti-fouling ability for the detection of cardiac marker troponin T

Publication year: 2011

Authors: J.T. Liu a, C.J. Chen b, T. Ikoma c, T. Yoshioka c, J.S. Cross d,e, S.-J.Chang a, J.-Z. Tsai b, J. Tanaka c

Published in: Analytica Chimica Acta, 2011, Vol. 703(1), p. 80-86

Designing a surface recognition layer with high anti-fouling ability, high affinity, and high specificity is an important issue to produce high sensitivity biosensing transducers. In this study, a self-assembled monolayer (SAM) consisting of a homogeneous mixture of oligo(ethylene glycol) (OEG)-terminated alkanethiolate and mercaptohexadecanoic acid (MHDA) on Au was employed for immobilizingtroponin T antibody and applied…

Peer-reviewed publications

Modification of cellulose films by adsorption of CMC and chitosan for controlled attachment of biomolecules

Publication year: 2011

Authors: Hannes Orelma 1, Ilari Filpponen 2, Leena-Sisko Johansson 3, Janne Laine 4, Orlando Jose Rojas 5

Published in: Biomacromolecules, 2011, Vol. 12 (12), p. 4311–4318

The adsorption of human immunoglobulin G (hIgG) and bovine serum albumin (BSA) on cellulose supports were investigated. The dynamics and extent of related adsorption processes were monitored by surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D). Amine groups were installed on the cellulose substrate by adsorption of chitosan from aqueous solution,…

Peer-reviewed publications

Adsorption of PEO-PPO-PEO Triblock Copolymers with End-Capped Cationic Chains of Poly(2-dimethylaminoethyl methacrylate)

Publication year: 2011

Authors: Xiaomeng Liu†, Arja-Helena Vesterinen§, Jan Genzer‡, Jukka V. Seppälä§, and Orlando J. Rojas*†∥

Published in: Langmuir, 2011, Vol. 27 (16), p. 9769–9780

We study the adsorption of a symmetric triblockcopolymer of ethylene oxide, EO, and propylene oxide, PO, end-capped with quarternized poly(2-dimethylaminoethylmethacrylate), DMAEMA (DMAEMA24EO132PO50EO132DMAEMA24). Light scattering and tensiometry are used tomeasure the relative size of the associated structures and surfaceexcess at the airliquid interface. The adsorbed amount, theamount of coupled water, and the viscoelasticity of the adsorbedpolymer…

Peer-reviewed publications

Surface Plasmon Resonance on Nanoscale Organic Films

Publication year: 2010

Authors: Willem M. Albers and Inger Vikholm-Lundin

Published in: Nano-Bio-Sensing, 1st ed, Chapter 4, Springer 2010

Surface Plasmon Resonance (SPR) has evolved over the past 20 years into a very versatile detection method, particularly in biosci- ence applications. Not only the scientific literature has greatly expanded, but also various commercial vendors of instrumentation, detection chips and reagents have emerged. In the scientific sphere the accent lies more and more on fabrication…

Peer-reviewed publications

Surface plasmon resonance instrument as a refractometer for liquids and ultrathin films

Publication year: 2010

Authors: H.Liang a,b, H.Miranto b,c, N.Granqvist b,d, J.W. Sadowski c, T.Viitala b, B.Wang a, M.Yliperttula b

Published in: Sensors and Actuators B: Chemical 2010, 149(1), 212-220

A surface plasmon resonance (SPR) setup in Kretschmann configuration is being utilized as a refractometer for both liquids as well as ultrathin films. The SPR signal detection technology used is based on a goniometer approach providing a wide angular scan range which facilitates highly accurate liquid and gas phase measurements. Attention was paid to improve…

Peer-reviewed publications

The Development of a diagnostic test for the detection of drugs in saliva using a disposable sample preparation Microfluidic cartridge

Publication year: 2010

Authors: Sesay A M.1, Krühne U.2, Sonny S.3, Lund Olesen T.4, Virtanen V.5

Published in: µTAS 2010 Groningen (2010), Verpoorte, S. et al.(Eds.), 1520-1522pp ISBN 978-0-9798064-3-8

In this paper we report on the development of a diagnostic test for the detection of illegal drugs in saliva using a disposable micro-fluidic sample preparation cartridge which was fabricated using CO2 and excimer laser ablation of a PMMA substrate assembly. The detection method is by immunoassay sensing on a surface plasmon resonance (SPR) platform.…

Peer-reviewed publications

Development of diagnostic SPR based biosensor for the detection of pharmaceutical compounds in saliva

Publication year: 2010

Authors: Susanna Sonny, Adama M. Sesay, Vesa Virtanen

Published in: Proc. SPIE 7376, Laser Applications in Life Sciences, 737605

The aim of the study is to develop diagnostic tests for the detection of pharmaceutical compounds in saliva. Oral fluid is increasingly being considered as an ideal sample matrix. It can be collected non-invasively and causes less stress to the person being tested. The detection of pharmaceutical compounds and drugs in saliva can give valuable…

Peer-reviewed publications

Affinity Interaction between Hexamer Peptide Ligand HWRGWV and Immunoglobulin G Studied by Quartz Crystal Microbalance and Surface Plasmon Resonance

Publication year: 2010

Authors: Fei Sen; Under the direction of Dr. Ruben G. Carbonell

Published in: Ph.D Dissertation, 2010, Chemical Engineering

Immunoglobulins (Ig), also referred to as antibodies, act as protective agents against pathogens trying to invade an organism. Human immunoglobulin G (hIgG), as the most prominent immunoglobulin presented in serum and other human fluids, has broad applications in fields like immunotherapy and clinical diagnostics. Staphylococcus aureus Protein A and Streptococcus Protein G are the most…

Peer-reviewed publications

Adsorption of a Nonionic Symmetric Triblock Copolymer on Surfaces with Different Hydrophobicity

Publication year: 2010

Authors: Xiaomeng Liu†, Dong Wu‡, Salomon Turgman-Cohen§, Jan Genzer§, Thomas W. Theyson∥ and Orlando J. Rojas*†⊥∥

Published in: Langmuir 2010, 26(12), 9565–9574

This study investigates the adsorption of a symmetric triblock nonionic polymer comprising ethylene oxide (EO) and propylene oxide (PO) blocks (Pluronic P-105, EO37PO56EO37) on a range of substrates including hydrophobic, i.e., polypropylene (PP), poly(ethylene terephthalate) (PET), nylon, and graphite, and hydrophilic, i.e., cellulose and silica. The adsorption process and the structure of the hydrated adsorbed…