Accurate Correction of the “Bulk Response” in Surface Plasmon Resonance Sensing Provides New Insights on Interactions Involving Lysozyme and Poly(ethylene glycol)
Publication year: 2022
Authors: Svirelis J. 1, Andersson J. 1, Stradner A. 2, Dahlin A. 1
Published in: ACS Sensors, 2022, Vol. 7, p. 1175-1182
Surface plasmon resonance is a very well-established surface sensitive technique for label-free analysis of biomolecular interactions, generating thousands of publications each year. An inconvenient effect that complicates interpretation of SPR results is the “bulk response” from molecules in solution, which generate signals without really binding to the surface. Here we present a physical model for…