protein-protein interaction

“Clickable ” Polymer Brush Interfaces: Tailoring Monovalent to Multivalent Ligand Display for Protein Immobilization and Sensing

Publication year: 2022

Authors: Degirmenci A. 1, Yeter Bas G. 1, Sanyal R. 1 2, Sanyal A. 1 2

Published in: Bioconjugate Chemistry, 2022, Vol. 33, p.1672-1684

Facile and effective functionalization of the interface of polymer-coated surfaces allows one to dictate the interaction of the underlying material with the chemical and biological analytes in its environment. Herein, we outline a modular approach that would enable installing a variety of “clickable” handles onto the surface of polymer brushes, enabling facile conjugation of various…

Peer-reviewed publications

Computer-Aided Engineering of Staphylokinase Toward Enhanced Affinity and Selectivity for Plasmin

Publication year: 2022

Authors: Nikitin D. 1 2, Mican J. 1 2 3, Toul M. 1 2, Bednar D. 1 2, Peskova M. 4 5, Kittova P. 4 6, Thalerova S. 1 4 5, Vitecek J. 1 4 3, Damborsky J. 1 2, Mikulik R. 1 3, Fleishman S. 7, Prokop Z. 1 2, Marek M. 1 2

Published in: Computational and Structural Biotechnology Journal, 2022, Vol. 20 p. 1366-1377

Cardio- and cerebrovascular diseases are leading causes of death and disability, resulting in one of the highest socio-economic burdens of any disease type. The discovery of bacterial and human plasminogen activators and their use as thrombolytic drugs have revolutionized treatment of these pathologies. Fibrin-specific agents have an advantage over non-specific factors because of lower rates…

Peer-reviewed publications

Characterisation of a unique linker segment of the Plasmodium falciparum cytosol localised Hsp110 chaperone

Publication year: 2021

Authors: G. Chakafana a,b, P.T.Mudau a, T.Zininga a,c, A.Shonhai a

Published in: International Journal of Biological Macromolecules, 2021, Vol.180, p. 272-285

Plasmodium falciparum expresses two essential cytosol localised chaperones; PfHsp70-1 and PfHsp70-z. PfHsp70-z (Hsp110 homologue) is thought to facilitate nucleotide exchange function of PfHsp70-1. PfHsp70-1 is a refoldase, while PfHsp70-z is restricted to holdase chaperone function. The structural features delineating functional specialisation of these chaperones remain unknown. Notably, PfHsp70-z possesses a unique linker segment which could account for its distinct functions. Using recombinant forms…

Peer-reviewed publications

Supporting data on characterisation of linker switch mutants of Plasmodium falciparum heat shock protein 110 and canonical Hsp70

Publication year: 2021

Authors: Graham Chakafana a,b, Pertunia T. Mudau a, Tawanda Zininga a,c, Addmore Shonhai a

Published in: Data in Brief, 2021, Vol. 37, p. 107177

Here, we present data on characterisation of the linker of Plasmodium falciparum Hsp110 (PfHsp70-z) relative to the linker of canonical Hsp70s in support of a co-published article [1]. The linker of PfHsp70-z was switched with that of canonical Hsp70s, represented by PfHsp70–1 (cytosolic counterpart of PfHsp70-z) and E. coli Hsp70/DnaK. The datasets represent comparative analyses…

Peer-reviewed publications

Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance

Publication year: 2014

Authors: Islam N. 1, Shen F. 1, Gurgel P.V. 1, Rojas O.J. 2, Carbonell R.G. 3

Published in: Biosensors and Bioelectronics, Vol. 58, 2014, p. 380-387

This paper characterizes the potential of novel hexameric peptide ligands for on-line IgG detection in bioprocesses. Surface Plasmon Resonance (SPR) was used to study the binding of human IgG to the hexameric peptide ligand HWRGWV, which was covalently grafted to alkanethiol self-assembled monolayers (SAM) on gold surfaces. Peptide coupling on SAMs was verified, followed by…