Aptamer-Assisted Protein Orientation on Silver Magnetic Nanoparticles: Application to Sensitive Leukocyte Cell-Derived Chemotaxin 2 Surface Plasmon Resonance Sensors
Leukocyte cell-derived chemotaxin 2 (LECT2) has been proved to be a potential biomarker for the diagnosis of liver fibrosis. In this work, a sensitive surface plasmon resonance (SPR) assay for LECT2 analysis was developed. Tyrosine kinase with immune globulin-like and epidermal growth factor-like domains 1 (Tie1) is an orphan receptor of LECT2 with a C-terminal…
Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance
The mechanical properties of biological nanoparticles play a crucial role in their interaction with the cellular membrane, in particular for cellular uptake. This has significant implications for the design of pharmaceutical carrier particles. In this context, liposomes have become increasingly popular, among other reasons due to their customizability and easily varied physicochemical properties. With currently…
Nanoparticles in Pharmacotheraphy Chapter 9B
Considering the enormous therapeutic benefits associated with the use of drug carriers of active substances, many researchers are currently focused on the development of targeted nanocarriers such as inorganic particles, synthetic polymers, or natural biopolymers. In recent years, enormous progress has been made through the development of new materials that can act as carriers of controllable properties such as size,…
Self-assembled hybrid nanocomposite films of carbon dots and hydrolyzed collagen
In the present work, hydrophilic carbon dots (CD) prepared by cotton cellulose were used in the building of self-assembled films with hydrolyzed collagen (CL) to verify their properties, especially molecular interactions and morphology. The materials were characterized by several techniques, such as AFM, FTIR and UV–Vis Spectroscopy, Surface Plasmon Resonance (SPR) and Calorimetric Titration. The…
Antimicrobial alumina nanobiostructures of disulfide- and triazole-linked peptides: Synthesis, characterization, membrane interactions and biological activity
Due to the its physical-chemical properties, alumina nanoparticles have potential applications in several areas, such as nanobiomaterials for medicinal or orthodontic implants, although the introduction of these devices poses a serious risk of microbial infection. One convenient strategy to circumvent this problem is to associate the nanomaterials to antimicrobial peptides with broad-spectrum of activities. In…
Layer-by-Layer nanostructured films of magnetite nanoparticles and polypyrrole towards synergistic effect on methylparaben electrochemical detection
The sustained use of cosmetics, hygiene products and medicines containing methylparaben (MeP) can be related to cases of cancer in women and children, which is why it is important to develop simple ways to monitor this compound. Gold electrodes were modified with Layer-by-Layer (LbL) films of magnetite nanoparticles (MNP) and polypyrrole (Ppy) for electrochemical determination…
Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-co-glycolic Acid Nanoparticles for Oral Colon Targeting
The exploitation of curcumin for oral disease treatment is limited by its low solubility, poor bioavailability, and low stability. Surface-functionalized poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) have shown promising results to ameliorate selective delivery of drugs to the gastrointestinal tract. In this study, curcumin-loaded PLGA NPs (CPLGA NPs) of about 200 nm were surface-coated with chitosan (CS) for gastro-intestinal mucosa adhesion, wheat germ agglutinin…
Real-Time Label-Free Monitoring of Nanoparticle Cell Uptake
The surface plasmon resonance technique in combination with whole cell sensing is used for the first time for real-time label-free monitoring of nanoparticle cell uptake. The uptake kinetics of several types of nanoparticles relevant to drug delivery applications into HeLa cells is determined. The cell uptake of the nanoparticles is confirmed by confocal microscopy. The…
Non-labelled surface sensitive techniques as platforms for pharmaceutical nanotechnology research
Insufficient delivery of drugs to the target sites like tumors and cells has been a barrier for achieving satisfying therapeutic effects in many diseases. Distribution and exposure of drugs to normal and healthy tissues may enhance the possibility of side effects and toxicity in vivo. Nanoparticle (NP) drug delivery systems have been developed to enable…
Real-Time Label-Free Monitoring of Drug- or Nanoparticle-Cell Interactions
Efficient drug delivery is one of the major challenges in modern pharmaceutical research. There is a constant need for developing new formulation strategies for new drug delivery systems because new chemical entities tend to have low solubility, and biotechnology drugs (e.g., DNA, oligonucleotides, antibodies, and proteins) permeate poorly to the target sites, limiting or even…