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MP-SPR keyword: CMD sensor slide

Peer-reviewed publications

Different Oligomeric States of the Tumor Suppressor p53 Show Identical Binding Behavior Towards the S100beta Homodimer

Publication year: 2022
Authors: Wei A. 1, Iacobucci C. 1, Schultze W. 1, Ihling C. 1, Arlt C. 1, Sinz A. 1
Published in: ChemBioChem, 2022, Vol. 23, e202100665

The tumor suppressor protein p53 is a transcription factor that is referred to as the “guardian of the genome” and plays an important role in cancer development. P53 is active as a homotetramer; the S100β homodimer binds to the intrinsically disordered C-terminus of p53 affecting its transcriptional activity. The p53/S100β complex is regarded as highly…

Peer-reviewed publications

Computer-Aided Engineering of Staphylokinase Toward Enhanced Affinity and Selectivity for Plasmin

Publication year: 2022
Authors: Nikitin D. 1 2, Mican J. 1 2 3, Toul M. 1 2, Bednar D. 1 2, Peskova M. 4 5, Kittova P. 4 6, Thalerova S. 1 4 5, Vitecek J. 1 4 3, Damborsky J. 1 2, Mikulik R. 1 3, Fleishman S. 7, Prokop Z. 1 2, Marek M. 1 2
Published in: Computational and Structural Biotechnology Journal, 2022, Vol. 20 p. 1366-1377

Cardio- and cerebrovascular diseases are leading causes of death and disability, resulting in one of the highest socio-economic burdens of any disease type. The discovery of bacterial and human plasminogen activators and their use as thrombolytic drugs have revolutionized treatment of these pathologies. Fibrin-specific agents have an advantage over non-specific factors because of lower rates…

Peer-reviewed publications

Characterisation of a unique linker segment of the Plasmodium falciparum cytosol localised Hsp110 chaperone

Publication year: 2021
Authors: G. Chakafana a,b, P.T.Mudau a, T.Zininga a,c, A.Shonhai a
Published in: International Journal of Biological Macromolecules, 2021, Vol.180, p. 272-285

Plasmodium falciparum expresses two essential cytosol localised chaperones; PfHsp70-1 and PfHsp70-z. PfHsp70-z (Hsp110 homologue) is thought to facilitate nucleotide exchange function of PfHsp70-1. PfHsp70-1 is a refoldase, while PfHsp70-z is restricted to holdase chaperone function. The structural features delineating functional specialisation of these chaperones remain unknown. Notably, PfHsp70-z possesses a unique linker segment which could account for its distinct functions. Using recombinant forms…

Peer-reviewed publications

Kinetic and thermodynamic insights into interaction of albumin with piperacillin: Spectroscopic and molecular modeling approaches

Publication year: 2019
Authors: Farzaneh Fathi a, Maryam Sharifi b, c, d, Amir Jafari e, Naser Kakavandi f, Soheila Kashanian g, h, Jafar Ezzati Nazhad Dolatabadi c, *, Mohammad-Reza Rashidi c, i, **
Published in: Journal of Molecular Liquids

The current study investigated the binding of BSA and piperacillin at multifarious concentrations (1 e128 mM) along with four different temperatures. To do this, three sensitive methods were employed including surface plasmon resonance (SPR), fluorescence spectroscopy and molecular docking (MD). In the SPR method, BSA immobilized on the carboxymethyl dextran (CMD) hydrogel sensor chip through NHS/EDC activation. The…