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MP-SPR keyword: biosensor

Peer-reviewed publications

An SPR-based method for Hill coefficient measurements: the case of insulin-degrading enzyme

Publication year: 2022
Authors: Distefano A. 1, Zingale G. 1, Grasso G. 2
Published in: Analytical and Bioanalytical Chemistry, 2022, Vol. 414 p. 4793-4802.

Insulin-degrading enzyme (IDE) is a highly conserved zinc metallopeptidase and is capable to catalytically cleave several substrates besides insulin, playing a pivotal role in several different biochemical pathways. Although its mechanism of action has been widely investigated, many conundrums still remain, hindering the possibility to rationally design specific modulators which could have important therapeutical applications…

Peer-reviewed publications

Rapid and sensitive detection of PD-L1 exosomes using Cu-TCPP 2D MOF as a SPR sensitizer

Publication year: 2022
Authors: Wang Y. a, Mao Z. b, Chen Q. c, Koh K. d, Hu X. a **, Chen H. a *
Published in: Biosensors and Bioelectronics, 201, 113954

Two-dimensional metal organic framework (2D MOF Cu-TCPP) with significantly enhanced photoelectric properties was synthesized by a simple hydrothermal method. The π-stacked electroactive porphyrin molecules of TCPP-based 2D MOF carry out charge transport in the MOF structure. The d-d band transition of Cu2+ and its 2D ultra-thin characteristics can produce excellent near-infrared light absorption to couple with SPR. Three key parameters including the refractive index sensitivity, detection…

Peer-reviewed publications

Spin coated chitin films for biosensors and its analysis are dependent on chitin-surface interactions

Publication year: 2018
Authors: Casteleijn M. G. a, Richardson D. a, Parkkila P. a, Granqvist N. b, Urtti A. a, c, Viitala T. a
Published in: Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2018, Vol. 539, p. 261-272

Chitin, abundant in nature, is a renewable resource with many possible applications in bioengineering. Biosensors, capable of label-free and in-line evaluation, play an important role in the investigation of chitin synthesis, degradation and interaction with other materials. This work presents a comparative study of the usefulness of a chitin surface preparation, either on gold (Au)…

Peer-reviewed publications

Functional Plasma Polymerized Surfaces for Biosensing

Publication year: 2020
Authors: Makhneva* E., Barillas L 1., Farka Z. 2, Pastucha M. 2, Skládal P. 2, Weltmann K.D. 1, Fricke K. 1
Published in: Applied Material Interfaces, 2020, Vol.12, 14, p.17100–17112

The capabilities of biosensors for fast, economic, and user-friendly analysis of complex samples has led to the exploitation of analytical devices for detection, quantification, and monitoring of specific chemical species for various applications. For a sufficiently high surface reactivity toward the adopted bioreceptors, a thin functional layer is required to enable coupling of the target…

Peer-reviewed publications

On the Importance of Controlling Film Architecture in Detecting Prostate Specific Antigen

Publication year: 2018
Authors: Graça J. S. a, Miyazaki C. M. a, Shimizu F. M. b, Volpati D. c, Mejía-Salazar J. R. b, Oliveira O.N. Jr. b, Ferreira M. a
Published in: Applied Surface Science, 2018, Vol. 434, p. 1175-1182

Immunosensors made with nanostructured films are promising for detecting cancer biomarkers, even at early stages of the disease, but this requires control of film architecture to preserve the biological activity of immobilized antibodies. In this study, we used electrochemical impedance spectroscopy (EIS) to detect Prostate Specific Antigen (PSA) with immunosensors produced with layer-by-layer (LbL) films…

Peer-reviewed publications

Grafting hyaluronic acid onto gold surface to achieve low protein fouling in surface plasmon resonance biosensors

Publication year: 2014
Authors: Liu X., Huang R., Su R., Qi W., Wang L., He Z.
Published in: ACS Applied Materials and Interfaces, 2014, Vol. 6(15), p. 13034-42

Antifouling surfaces capable of reducing nonspecific protein adsorption from natural complex media are highly desirable in surface plasmon resonance (SPR) biosensors. A new protein-resistant surface made through the chemical grafting of easily available hyaluronic acid (HA) onto gold (Au) substrate demonstrates excellent antifouling performance against protein adsorption. AFM images showed the uniform HA layer with…

Peer-reviewed publications

Specificity and regenerability of short peptide ligands supported on polymer layers for immunoglobulin G binding and detection

Publication year: 2013
Authors: Zhang Y. 1, Islam N. 2, Carbonell R.G. 2, Rojas O.J. 1,2
Published in: ACS Applied Materials and Interfaces, 2013, Vol.5 (16), p. 8030-7

We demonstrate the specificity, regenerability, and excellent storage stability of short peptide-based systems for detection of immunoglobulin G (IgG). The bioactive component consisted of acetylated-HWRGWVA (Ac-HWRGWVA), a peptide with high IgG binding affinity, which was immobilized onto copolymer matrixes of poly(2-aminoethyl methacrylate hydrochloride-co-2-hydroxyethyl methacrylate) (poly(AMA-co-HEMA)). Surface plasmon resonance (SPR) and quartz crystal microgravimetry (QCM) were…

Peer-reviewed publications

Surface plasmon resonance analysis for detecting non-structural protein 1 of dengue virus in Indonesia

Publication year: 2020
Authors: Widoretno 1 2, Sjahrurachman A. 3, Dewi B.E. 4, Lischer K. 5 6, Pratami D.K. 7, Flamandita D. 5, Sahlan M. 5 6
Published in: Saudi Journal of Biological Sciences, 2020, Vol. 8, p.1931-1937

Dengue is an acute febrile disease caused by dengue virus (DENV) that is transmitted by Aedes sp., which causes serious health conditions in many countries. Non-structural protein 1 (NS1) is a co-factor for the RNA replication of this virus, which represents a new strategy for the identification of dengue. Prompt and accurate laboratory diagnosis of this infection…

Peer-reviewed publications

Functionalized Terpolymer-Brush-Based Biointerface with Improved Antifouling Properties for Ultra-Sensitive Direct Detection of Virus in Crude Clinical Samples

Publication year: 2021
Authors: Michala Forinová* a., Alina Pilipenco a. , Ivana Vísov̌á a., N. Scott Lynn a., Jr, Jakub Dostálek a.,b., Hana Masková c., Václav Hönig d., e., Martin Palus, d. Martin Selinger c., d., Pavlína Kocov̌á c., Filip Dycka c., Jan Sterba c., Milan Houska a., Markéta Vrabcová a., Petr Horák a., Judita Anthi a., Chao-Ping Tung f., Chung-Ming Yu f., Chi-Yung Chen f., Yu-Chuan Huang f., Pei-Hsun Tsai f., Szu-Yu Lin f., Hung-Ju Hsu f., An-Suei Yang f., Alexandr Dejneka a., and Hana Vaisocherová-Lísalová* a.
Published in: ACS Applied Materials & Interfaces, 13, 60612 – 60624

New analytical techniques that overcome major drawbacks of current routinely used viral infection diagnosis methods, i.e., the long analysis time and laboriousness of real-time reverse-transcription polymerase chain reaction (qRT-PCR) and the insufficient sensitivity of “antigen tests”, are urgently needed in the context of SARS-CoV-2 and other highly contagious viruses. Here, we report on an antifouling…

Peer-reviewed publications

Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor

Publication year: 2020
Authors: Macchia E. 12, Sarcina L. 1, Picca R.A. 1, Manoli K. 1, Di Franco C. 1, Scamarcio G. 34 Torsi L. 125
Published in: Analytical and Bioanalytical Chemistry, 2020, Vol. 412, p. 811–818

Early diagnosis of the infection caused by human immunodeficiency virus type-1 (HIV-1) is vital to achieve efficient therapeutic treatment and limit the disease spreading when the viremia is at its highest level. To this end, a point-of-care HIV-1 detection carried out with label-free, low-cost, and ultra-sensitive screening technologies would be of great relevance. Herein, a…