Siirry sisältöön

MP-SPR keyword: binding kinetics

Peer-reviewed publications

Computer-Aided Engineering of Staphylokinase Toward Enhanced Affinity and Selectivity for Plasmin

Publication year: 2022
Authors: Nikitin D. 1 2, Mican J. 1 2 3, Toul M. 1 2, Bednar D. 1 2, Peskova M. 4 5, Kittova P. 4 6, Thalerova S. 1 4 5, Vitecek J. 1 4 3, Damborsky J. 1 2, Mikulik R. 1 3, Fleishman S. 7, Prokop Z. 1 2, Marek M. 1 2
Published in: Computational and Structural Biotechnology Journal, 2022, Vol. 20 p. 1366-1377

Cardio- and cerebrovascular diseases are leading causes of death and disability, resulting in one of the highest socio-economic burdens of any disease type. The discovery of bacterial and human plasminogen activators and their use as thrombolytic drugs have revolutionized treatment of these pathologies. Fibrin-specific agents have an advantage over non-specific factors because of lower rates…

Peer-reviewed publications

Oligomerization Alters Binding Affinity Between Amyloid Beta and a Modulator of Peptide Aggregation

Publication year: 2017
Authors: Hilt S. 1, Rojalin T. 2, 3, Viitala T. 3, Koivuniemi A.3, Bunker A. 3, Wachsmann-Hogiu S. 2, 4, Kálai T. 5, Hideg K. 5, Yliperttula M. 3, 6, Voss J. C. 1
Published in: The Journal of Physical Chemistry C, 2017, Vol. 121 (43), p. 23974–23987

The soluble oligomeric form of the amyloid beta (Aβ) peptide is the major causative agent in the molecular pathogenesis of Alzheimer’s disease (AD). We have previously developed a pyrroline-nitroxyl fluorene compound (SLF) that blocks the toxicity of Aβ. Here we introduce the multiparametric surface plasmon resonance (MP-SPR) approach to quantify SLF binding and its effect…

Peer-reviewed publications

Taste masking effect of chlorogenic acid (CGA) on bitter drugs evaluated by taste sensor and surface plasmon resonance on the basis of CGA-Drug interactions

Publication year: 2017
Authors: Shiraishi S.1, Haraguchi T., Nakamura S., Li D., Kojima H., Yoshida M., Uchida T.
Published in: Chemical and Pharmaceutical Bulletin, Vol. 65 (2017), No. 2, p. 127-13

The purpose of this study was to evaluate the taste-masking effects of chlorogenic acid (CGA) on bitter drugs using taste sensor measurements and surface plasmon resonance (SPR) analysis of CGA-drug interactions. Six different bitter drugs were used: amlodipine besylate (AMD), diphenhydramine hydrochloride (DPH), donepezil hydrochloride (DNP), rebamipide (RBM), diclofenac sodium (DCF) and etodolac (ETD). Taste…

Peer-reviewed publications

Kinetics of PKCe Activating and Inhibiting Llama Single Chain Antibodies and Their Effect on PKCe Translocation in HeLa Cells

Publication year: 2012
Authors: Milla Summanen 1,2, Niko Granqvist 3, Raimo K. Tuominen 2, Marjo Yliperttula 3, C.Theo Verrips 1, Johannes Boonstra 1; Christophe Blanchetot 1, Elina Ekokoski 2
Published in: PLos ONE, 2012, Vol. 7, Issue 4, e3563

Dysregulation of PKCe is involved in several serious diseases such as cancer, type II diabetes and Alzheimer’s disease.Therefore, specific activators and inhibitors of PKCe hold promise as future therapeutics, in addition to being useful inresearch into PKCe regulated pathways. We have previously described llama single chain antibodies (VHHs) that specificallyactivate (A10, C1 and D1) or…