Assembly of citrate gold nanoparticles on hydrophilic monolayers
Self-assembled monolayers (SAMs) as model surfaces were linked onto planar gold films thorough lipoic acid or disulfide groups. The molecules used were polyethylene glycol (EG-S-S), N-[tris-(hydroxymethyl)methyl]acrylamide polymers with and without lipoic acid (Lipa-pTHMMAA and pTHMMAA) and a lipoic acid triazine derivative (Lipa-MF). All the layers, but Lipa-MF with a primary amino group were hydroxyl terminated.…
Impact of polysaccharide and protein interactions on membrane fouling: Particle deposition and layer formation
Membrane fouling, which limits the application of membrane bioreactors, has received considerable research attention in recent years. In this work, filtration modeling was performed in combination with surface plasmon resonance (SPR) analysis to investigate the membrane fouling mechanism. Sodium alginate (SA) and bovine serum albumin (BSA) were used to perform dead-end filtration on hydrophilic and…
Adsorption and Conformation Behavior of Lysozyme on a Gold Surface Determined by QCM-D, MP-SPR, and FTIR
The physicochemical properties of protein layers at the solid–liquid interface are essential in many biological processes. This study aimed to link the structural analysis of adsorbed lysozyme at the water/gold surface at pH 7.5 in a wide range of concentrations. Particular attention was paid to the protein’s structural stability and the hydration of the protein…
Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy
Interactions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively in the past, while polysaccharide-lectin interactions have scarcely been investigated. Here, we explore the adsorption of galactomannans (GM) extracted from Prosopis affinis on cellulose thin films determined by a combination of multi-parameter surface…
PEDOT-polyamine composite films for bioelectrochemical platforms -flexible and easy to derivatize
We report a straightforward route for the preparation of flexible, electrochemically stable and easily functionalizable poly(3,4-ethylenedioxythiophene) (PEDOT) composite films deposited on PET foils as biosensing platforms. For this purpose, poly(allylamine) hydrochloride (PAH) was blended with PEDOT to provide amine-bearing sites for further biofunctionalization as well as to improve the mechanical properties of the films. The…
Generic High-Capacity Electrochemical Capture and Release of Proteins by Polyelectrolyte Brushes
Capture and release of proteins by an electrical signal is of general interest but methods have proven difficult to develop. We present a polyelectrolyte brush electrode with high-capacity (several µg/cm2 ) for protein immobilization that captures and releases proteins by an electrochemical potential. The electrochemical potential produces an interfacial pH gradient that enables rapid and…
Layer-by-layer assembly of lysozyme with iota-carrageenan and gum Arabic for surface modification of food packaging materials with improved barrier properties
The study aimed to investigate the surface modification of biaxially oriented polypropylene (BOPP) by layer-by-layer (LbL) assembly of lysozyme (LZ) with two different polysaccharides, iota-carrageenan (IC) and gum arabic (GA), for food packaging applications. The effects of solution pH, adsorption time, elimination of intermediate drying steps were investigated. The LbL film growth was monitored up…
Unraveling the Mechanism and Kinetics of Binding of an LCI-eGFP-Polymer for Antifouling Coatings
The ability of proteins to adsorb irreversibly onto surfaces opens new possibilities to functionalize biological interfaces. Herein, the mechanism and kinetics of adsorption of protein-polymer macromolecules with the ability to equip surfaces with antifouling properties are investigated. These macromolecules consist of the liquid chromatography peak I peptide from which antifouling polymer brushes are grafted using…
Adhesion and Structural Changes of PEGylated Lipid Nanocarriers on Silica Surfaces
PEGylated lipid nanoparticles have a continuously expanding range of applications, particularly within pharmaceutical areas. Hereby, it is shown with the help of the Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and other surface sensitive techniques that, at room temperature, PEGylated liposomes and lipodisks adhere strongly to silica surfaces resulting in the displacement of the hydration…
Rapid and Oriented Immobilization of Laccases on Electrodes via a Methionine-Rich Peptide
Different from other laccases, copper efflux oxidase (CueO) from Escherichia coli possesses an additional methionine-rich segment (MetRich), which is generally considered to be detrimental to its oxidase activity. Herein, we reveal that MetRich plays an important role in rapid immobilization of CueO on electrodes by studying the adsorption and bioelectrocatalysis behaviors of CueO, a truncated CueO (ΔMetRich…