Why choose MP-SPR for biophysics?

No artifacts from lipid swelling

Label-free measurements of lipids are typically hindered by artifacts from swelling (traditional SPR) or high liquid content (QCM) when measuring lipids in water or solvent. Uniquely, MP-SPR allows the measurement of environmental factors using PureKinetics™ feature and thus provides high-quality data.

Easy lipid membrane formation

Since our MP-SPR instruments use an advanced elastomeric coating on the prism, we can work with simple coated glass slides as measurement substrates. This allows the substrates to be modified ex-situ. The extended scanning angular range also allows for variety of substrate coatings. This in turns allows versatile methods to be used for deposition of phospolipid membranes for MP-SPR studies. The most typical ones are liposomes on hydrogels and supported lipid bilayers on SiO2.

Our gold sensors can be re-used even after work with lipids thanks to a special adhesion layer. Typical cleaning protocols are provided to our users in our user intranet.


Membrane quality

Before injecting any valuable samples, the quality of the lipid deposition can be checked and quantified. MP-SPR with LayerSolver™ software and measurements with two wavelengths enable true thickness and optical density measurement. This provides invaluable information about the quality of the lipid membrane and its conformation. 

In fact, thickness and refractive index of the layer can be measured at the same time with the interaction kinetics. This in turn can show for instance lipid rupture or swelling of layers in real-time.

Combination of MP-SPR with electrochemistry

It is possible to combine MP-SPR with electrical stimulation of the biomembrane or with recording of the electrical potential changes of the membrane. The MP-SPR instruments can be used with a number of different flow-cells, including electro-chemical flow-cell. We provide three different electrode types for different measurement needs.

From membranes to living cells

To maintain their functionality, some of the membrane proteins require also presence of other cellular structures. Also, certain membrane proteins are difficult to keep in functional form after retrieval from the cell. Therefore there is a need to move to label-free measurements on whole cells.

MP-SPR is currently the only platform that allows label-free separation of permeation of molecules across cell layer from internalization of drugs and nanoparticles by cells. 


Recommended MP-SPR instruments for biophysics:

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