MP-SPR in biophysics

Interfaces and membranes such as the cell phospholipid membrane and mitochondrial membrane have important biochemical and biophysical roles aside of being barriers. Moreover, membranes contribute as microenvironment to synthesis and catalysis reactions, and the cell membrane also incorporates all the cell signaling components.

The biological biomembranes and interactions of analysts with biomembranes are studied in drug development, nanoparticle biological interaction, targeted drug delivery, gene therapy and personalized medicine. Biomembranes are often of great interest in fundamental biochemistry and biophysics research.

See five reasons to choose MP-SPR for biophysics:

  • No artifacts from lipid swelling
  • Easy lipid membrane formation on variety of substrates
  • Membrane quality assessment
  • Combination of MP-SPR with electrochemistry
  • From measurements on biomembranes to measurements on living cells

See five key questions in biophysics that MP-SPR can answer:

  • What is the kinetics of supported lipid bilayer formation?
  • How does nanoparticle X interact with a biomembrane?
  • How does a membrane protein (such as GPCR) interact with a drug?
  • What is the quality (thickness and optical density) of the biomembrane?
  • How stable is a membrane system in air?

See selected Application Notes to see that MP-SPR works:

  • AN#167 Capture of T-cells and analysis of membrane receptor affinity with biofunctionalized lipid sensor
  • AN#164 Affinity and kinetics of extracellular vesicles – protein interaction
  • AN#157 Detection of toxin induced pore formation in membranes
  • AN#152 Drug delivery nanocarrier studies using MP-SPR
  • AN#151 MP-SPR measurements of soft and hard corona on nanoparticle in 100% serum
  • AN#147 Analyzing dissociation kinetics of IgG from protein A using MP-SPR and PureKinetics™
  • AN#139 Liposomes attachment on the sensor surface – thickness and refractive index ensure the layer conformation
  • AN#137 Drug interaction with cell monolayer measured with MP-SPR

See the animation of MP-SPR characterization of nanoparticle interaction with a lipid bilayer and with liposomes!

 Selected publications:

What our users say about MP-SPR for lipid measurements:

"We use MP-SPR also in our drug-lipid interaction work. It is important for us that we are able to check the quality of the lipid membrane before introducing any expensive samples." Dr. Tapani Viitala, University of Helsinki, Finland