Combining MP-SPR and electrochemistry in material sciences

Five typical research areas to combine electrochemistry and MP-SPR:

• Electroswithchable reactions and functional biomaterials
• Real-time measurements of conformational changes
• Development of simple electrochemical sensors in one device - more information here
• Supported lipid membrane quality assessment - more information here
• Catalytical bioreactions

See reasons to choose MP-SPR for switchable material development: 

• Easy ex-situ modification of SPR surfaces
• Simultaneous recording of electrochemical and MP-SPR signals
• Compatible with most organic solvents
• Conformational changes in real-time
• No swelling artifacts

See five key questions in electroswitchable material development that MP-SPR can answer:

• How fast does protein X adsorb onto surface A?
• How does the reaction change at different temperature, pH and electric potential?
• How fast is the electrodeposition?
• How fast is the catalysis?
• How much liquids can the material uptake and release at different pH?

See selected Application Notes to see that MP-SPR works:

• AN#143 Real-time monitoring of antibody binding by parallel MP-SPR, potentiometry and impedance spectroscopy
• AN#142 Real-time monitoring of metal stripping and deposition with EC-MP-SPR
• AN#140 Self-assembly of gold nanoparticles measured with MP-SPR
• AN#136 Polymer collapse due to pH or electric potential change monitored with MP-SPR

Selected publications:

Electroswitchable surfaces:

• Malmström et al. Grafting from Poly(3,4-ethylenedioxythiophene): A Simple Route to Versatile Electrically Addressable Surfaces

Electrochemical sensor development using MP-SPR:

• Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection, Miyazaki et al., Materials Science and Engineering: C, Vol. 58: p. 310–315
• Effect of gold nanoparticles on the structure and electron-transfer characteristics of glucose oxidase redox polyelectrolyte-surfactant complexes, Cortez ML et al., Chemistry, 2014
• UV crosslinked poly(acrylic acid): a simple method to bio-functionalize electrolyte-gated OFET biosensors, Mulla MY et al., J. Mater. Chem. B, 2015, 3, p. 5049-5057
• SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis, Souto DE et al., Biosens Bioelectron. 2015 Aug 15;70:275-8
• More on electrochemical biosensor development

What our users say about MP-SPR for development of biomimetic surfaces:

Polymer collapse due to pH or electric potential change monitored with MP-SPR

Application Note #136

Figure 1. Polymer layers conformational changes in different pH. PAA= poly (acrylic acid), PEDOT= poly (3,4-ethylenedioxythiophene).

Multi-Parametric Surface Plasmon resonance (MP-SPR) was utilized to follow PAA polymer brushes swelling and collapse caused by pH or electric potential change. MP-SPR with electrochemical cell was utilized to form potential change to the conductive sensor surface. Multi wavelength MP-SPR measures whole SPR curve with two different wavelength and this can be utilized to calculate deposited layer thickness (for more details see AN#128).

Recommended instrument for this application:

200 OTSO	400 KONTIO	210A VASA	410A KAURIS	220A NAALI	420A ILVES

Self-assembly of gold nanoparticles measured with MP-SPR

Application Note #140

Whole SPR curves during gold nanoparticle deposition, measured with 785nm wavelength.

Gold nanoparticles were immobilized on a monolayer selfassembled on gold. Functional groups on the chain ends of the monolayer facilitated an anchoring of gold nanoparticles to the layer. Multi-Parametric Surface Plasmon Resonance (MP-SPR) enabled a real-time measurement of the binding of the gold nanoparticles to the surface layer.

Recommended instrument for this application:

200 OTSO	400 KONTIO	210A VASA	410A KAURIS	220A NAALI	420A ILVES

See five reasons to choose MP-SPR for electrochemical studies.