MP-SPR method can be used in conjunction with a variety of electrochemical techniques for gathering both optical and electrochemical real-time data simultaneously from the same reaction. Multiple electrochemical methods such as potentiometric, amperometric or impedance spectroscopy can be measured.
The potentiostat can be used for electrodeposition of thin films in-situ, to modify electrical potential across a surface to study biomimetic surfaces (electrochemical switching) or to gather information with a technology sensitive to other type of information than optical changes.
Electroswitchable surfaces:
Electrochemical sensor development using MP-SPR:
"We use MP-SPR to quantify conformation changes of proteins at the surface under different environmental conditions."
Prof. Barbara Jachimska, Polish Academy of Sciences, Poland
Figure 1. Polymer layers conformational changes in different pH. PAA= poly (acrylic acid), PEDOT= poly (3,4-ethylenedioxythiophene).
Multi-Parametric Surface Plasmon resonance (MP-SPR) was utilized to follow PAA polymer brushes swelling and collapse caused by pH or electric potential change. MP-SPR with electrochemical cell was utilized to form potential change to the conductive sensor surface. Multi wavelength MP-SPR measures whole SPR curve with two different wavelength and this can be utilized to calculate deposited layer thickness (for more details see AN#128).
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Whole SPR curves during gold nanoparticle deposition, measured with 785nm wavelength.
Gold nanoparticles were immobilized on a monolayer selfassembled on gold. Functional groups on the chain ends of the monolayer facilitated an anchoring of gold nanoparticles to the layer. Multi-Parametric Surface Plasmon Resonance (MP-SPR) enabled a real-time measurement of the binding of the gold nanoparticles to the surface layer.
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