MP-SPR is an invaluable tool for assay development in fields such as food and feed safety, environmental safety, clinical diagnostics, border control and process control. While part of the research worldwide is dedicated to high-throughput instruments, more and more attention is gained by portable or point-of-care instruments. MP-SPR is an excellent tool for development of portable biosensors. Point-of-care biosensors include typically electrochemical sensors, surface enhance Raman sensors (SERS), ELISA, fluorescence or newly also printed diagnostics.
For information on electrochemical biosensor development click here.
See five key questions in assay development that MP-SPR can answer:
For electrochemical sensor development using MP-SPR, click here.
Printed biosensor development using MP-SPR:
MEMS sensor development using MP-SPR:
Novel coatings to avoid non-specific binding using MP-SPR:
Assay development using MP-SPR:
Improving performance of optical biosensors:
"It is essential that we can prepare our own biosensing surfaces in-situ and ex-situ and test them with SPR."
Dr. Adama Sesay, Center for Measurement and Information Systems, University of Oulu, Finland
Figure 3. (A) MP-SPR signal response after injecting 1–20 μg/mL bio-CRP antigen over MuOH:Biotin-PEG-thiol (85:15 mol%)/streptavidin sensor surface.
MP-SPR is an effective reference tool for immunosensor development.
MP-SPR is utilized to determine affinity biosensor surface binding capacity of different parts of the immunosensor. Streptavidin binding capacity to the MBP-thiol SAM was 366 ± 2 ng/cm2 and Bio-CRP antigen binding capacity to the MBP-thiol /streptavidin surface was 105 ng/cm2. The MP-SPR also allowed for concentration optimization for the sensor system.
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In-situ preparation of chitosan and cyclodextrin-functionalized cellulose fiber yarns and capture of the synthetic estrogen hormone 17α-ethinyl estradiol (EE2) from water was investigated to address waste water purification. The thickness of functionalized chitosan and cyclodextrin layers were determined. The effectivity of the hormone capture was determined by determining the thickness of adsorbed EE2 layer.
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Figure 2. A compilation of the all the injections used in the experiment, concentrations ranging from 1000 nM to 0.1 nM.
dNA assays can be applied for detecting genetic material in order to detect genetic disorders, mutations, gene transfection or species from a large variety of samples. By assaying specific single stranded oligonucleotides of over 20 nucleotides in length, it is possible to detect and quantify unique gene sequences from large amounts of genetic material [1]. BioNavis SPR Navi 200 can be utilized easily into a dNA assay that can detect and quantify single-stranded oligonucleotides with high precision over a large concentration range from micro to nanomolar concentrations.
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Multi-Parametric Surface Plasmon Resonance (MP-SPR) instruments
can measure optical properties and address surface interactions from
very thin films (down to Ångstroms) up to micrometer thick layers.
The measurement of thicker sensor coatings is based on a SPR
waveguide mode.
Micrometer thick chitin layers were spin-coated onto gold (Au) and
polystyrene (PS) substrates and subsequently assessed through
MP-SPR waveguide signal. Thickness and refractive index (RI) of chitin
layer were determined in dry and liquid conditions using the same
instrument set-up (Figure 1). The polymer thickness was almost 2 μm
in air but less than 200 nm in buffer and in addition it was thicker on
gold when compared to polystyrene. The affinity constant of a chitin
binding domain of Bacillus circulans (CBD-INN) was determined and
evidenced to be higher on chitin deposited on the polystyrene-coated
sensor when compared to chitin on gold. Additionally, split-intein
protein (INC-eGFP) affinity on CBD-INN was determined.
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Whole SPR curves during gold nanoparticle deposition, measured with 785nm wavelength.
Gold nanoparticles were immobilized on a monolayer selfassembled on gold. Functional groups on the chain ends of the monolayer facilitated an anchoring of gold nanoparticles to the layer. Multi-Parametric Surface Plasmon Resonance (MP-SPR) enabled a real-time measurement of the binding of the gold nanoparticles to the surface layer.
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