Figure 3. (A) MP-SPR signal response after injecting 1–20 μg/mL bio-CRP antigen over MuOH:Biotin-PEG-thiol (85:15 mol%)/streptavidin sensor surface.

MP-SPR is an effective reference tool for immunosensor development.

MP-SPR is utilized to determine affinity biosensor surface binding capacity of different parts of the immunosensor. Streptavidin binding capacity to the MBP-thiol SAM was 366 ± 2 ng/cm2 and Bio-CRP antigen binding capacity to the MBP-thiol /streptavidin surface was 105 ng/cm2. The MP-SPR also allowed for concentration optimization for the sensor system.

Recommended instrument for this application:

200 OTSO	400 KONTIO	210A VASA	410A KAURIS	220A NAALI	420A ILVES

Figure 1. CNF coated with chitosan by adsorption. TO-βCD is conjugated to chitosan with EDC-NHS chemistry. TO-βCD captures EE2 from water.

In-situ preparation of chitosan and cyclodextrin-functionalized cellulose fiber yarns and capture of the synthetic estrogen hormone 17α-ethinyl estradiol (EE2) from water was investigated to address waste water purification. The thickness of functionalized chitosan and cyclodextrin layers were determined. The effectivity of the hormone capture was determined by determining the thickness of adsorbed EE2 layer.

Recommended MP-SPR instruments for this application:

200 OTSO	400 KONTIO	210A VASA	410A KAURIS	220A NAALI	420A ILVES

Figure 2. A compilation of the all the injections used in the experiment, concentrations ranging from 1000 nM to 0.1 nM.

dNA assays can be applied for detecting genetic material in order to detect genetic disorders, mutations, gene transfection or species from a large variety of samples. By assaying specific single stranded oligonucleotides of over 20 nucleotides in length, it is possible to detect and quantify unique gene sequences from large amounts of genetic material [1]. BioNavis SPR Navi 200 can be utilized easily into a dNA assay that can detect and quantify single-stranded oligonucleotides with high precision over a large concentration range from micro to nanomolar concentrations.

Recommended instrument for this application:

200 OTSO	400 KONTIO	210A VASA	410A KAURIS	220A NAALI	420A ILVES

Figure 3. Full SPR curves registered in air at two wavelengths (670 nm – orange lines, 785 nm – blue lines). (A) uncoated gold and (B) chitin layer on polystyrene and gold. Dashed lines are the fitted curves from LayerSolver™ analysis.

Multi-Parametric Surface Plasmon Resonance (MP-SPR) instruments can measure optical properties and address surface interactions from very thin films (down to Ångstroms) up to micrometer thick layers. The measurement of thicker sensor coatings is based on a SPR waveguide mode. Micrometer thick chitin layers were spin-coated onto gold (Au) and polystyrene (PS) substrates and subsequently assessed through MP-SPR waveguide signal. Thickness and refractive index (RI) of chitin layer were determined in dry and liquid conditions using the same instrument set-up (Figure 1). The polymer thickness was almost 2 μm in air but less than 200 nm in buffer and in addition it was thicker on gold when compared to polystyrene. The affinity constant of a chitin binding domain of Bacillus circulans (CBD-INN) was determined and evidenced to be higher on chitin deposited on the polystyrene-coated sensor when compared to chitin on gold. Additionally, split-intein protein (INC-eGFP) affinity on CBD-INN was determined.

Recommended MP-SPR instruments for this application:

200 OTSO	400 KONTIO	210A VASA	410A KAURIS	220A NAALI	420A ILVES

Whole SPR curves during gold nanoparticle deposition, measured with 785nm wavelength.

Gold nanoparticles were immobilized on a monolayer selfassembled on gold. Functional groups on the chain ends of the monolayer facilitated an anchoring of gold nanoparticles to the layer. Multi-Parametric Surface Plasmon Resonance (MP-SPR) enabled a real-time measurement of the binding of the gold nanoparticles to the surface layer.

Recommended instrument for this application:

200 OTSO	400 KONTIO	210A VASA	410A KAURIS	220A NAALI	420A ILVES