Interfaces and membranes such as the cell phospholipid membrane and mitochondrial membrane have important biochemical and biophysical roles aside of being barriers. Moreover, membranes contribute as microenvironment to synthesis and catalysis reactions, and the cell membrane also incorporates all the cell signaling components.
The biological biomembranes and interactions of analysts with biomembranes are studied in drug development, nanoparticle biological interaction, targeted drug delivery, gene therapy and personalized medicine. Biomembranes are often of great interest in fundamental biochemistry and biophysics research.
See five reasons to choose MP-SPR for biophysics:
See the animation of MP-SPR characterization of nanoparticle interaction with a lipid bilayer and with liposomes!
"We use MP-SPR also in our drug-lipid interaction work. It is important for us that we are able to check the quality of the lipid membrane before introducing any expensive samples." Dr. Tapani Viitala, University of Helsinki, Finland
Assessment of T-cell specificity to tumor associated antigens is crucial for the development of personalized immunotherapies against cancer. Multi-Parametric Surface Plasmon Resonance (MP-SPR) was applied for characterization of interaction of T-cell receptors (TCR) from tumor-specific CD8+ T-cells. Intact live cells were captured onto biomimetic surfaces composed of artificial cell membranes functionalized with peptide-major histocompatibility complexes (pMHC). Real-time affinity analysis of immunological synapse formation betweenTCR and pMHC was completed from 4 different T-cell populations.
![]() |
![]() |
![]() |
![]() |
Figure 2. Change in SPR peak minimum angle during lipid deposition, From left to right: SLB formation on SiO2 surface, SLV formation on PEG-SAM surface and SLB formation on 6kDa dextran surface.
Multi-Parametric Surface Plasmon Resonance (MP-SPR) was used to characterize lipid layer structures adsorbed on a different substrates. Silicon dioxide and low molecule weight dextran surfaces supported formation of lipid bilayer (SLB) whereas thiolated polyethylene glycol supported vesicular layer formation (SLV). Thickness and refractive index of deposited lipid layer was calculated: SLB was about 5nm thick layer whereas SLV was 10nm thick layer.
![]() |
![]() |
![]() |