MP-SPR is a sensitive platform to determine antibody and fragment binding on antigen. Label-free interactions are measured in real-time, revealing affinity and kinetics of the binding. MP-SPR instruments can also handle target molecules incorporated in liposomes (membrane extract) and living cells, enabling interaction measurements in natural-like environment.
Interaction measurements can be performed in diverse fluids including 100% serum, plasma or cell growth medium. With PureKinetics™, bulk effect can be distinguished from binding and thus high precision results are obtained. Fitting SPR curves with LayerSolver™ provides insight into the conformation and orientation of the antibodies via thickness assessment of the adsorbed layers.
Reasons to choose MP-SPR for antibody characterization:
- Label-free affinity and kinetics
- Low total cost of ownership
- Conformation changes
- Crude samples (100% serum)
- Biopharmaceuticals even from cell medium
- Easy ex-situ preparation of own sensor surfaces
- Robust fluidics to handle harsh chemicals
With MP-SPR:
- Determine an antibody’s affinity and kinetics to antigens
- Select the best antibody for successful immunoassays
- Measure antibody binding on receptor in lipid environment
- Characterize which cell signaling cascade an antibody activates in living cells
- Identify the monoclonal antibody with the highest affinity
- See the real binding without bulk effect

Not yet convinced? See selected Application Notes to see how MP-SPR works:
- AN#169 Diagnostic method for serological testing of Covid-19 antibodies
- AN#168 Regenerable avidin kit assays Part II: Biomolecule affinity and concentration studies
- AN#166 Regenerable avidin kit assays Part I: Working principle, antibody – antigen interaction
- AN#162 Concentration analysis of nutritionally important proteins from milk
- AN#147 Analyzing dissociation kinetics of IgG from protein A using MP-SPR and PureKinetics™