| Technique | Multi-Parametric Surface Plasmon Resonance (MP-SPR) |
| Measuring principle | True goniometric SPR with rotating laser (true angluar resolution 0.001o) |
| Wavelength of light | 670 nm (other wavelengths and set-ups such as several lasers per channel available by request) |
| SPR angle range | 40o - 78o |
| Prism coating | Optical gel index matching (no need for messy oil operation between measurements) |
| Sensor slides | 12 x 20mm, gold coated, superior adhesion (other coatings available as option) |
| Flow cell |
Standard: Easily exchangeable, 2 channels, PDMS material, 100µ cell height |
| Flow cell volume | 1 µl as standard |
| Sample volume | Min. 20 µl (or less through partial loopfill) |
| Refractive index (RI) range | 1.00 - 1.40 (Bulk) |
| Dynamic range | 38 000 mdeg |
| Noise level | 1 µRIU (1 x 10-6 of Refractive Index Unit, a physical measure) - corresponds to 1 pg/mm² surface coverage of a typical protein |
| Sensitivity |
1μRIU - corresponds to 50 ppm change in reflectance signal in the Fixed Angle mode; and to 0.15 mdeg change in SPR peak angular position in the Angular Scan mode |
| Fastest sampling rate | from 4 ms |
| Temperature control | 15-45oC (ambient +20/-7oC) |
| Environment and interface | SPR Navi control and data viewer software compatible with MS Windows® XP / VISTA / 7 (however, does not support Starter editions used in netbooks), USB 2.0 |
| Power requirements | 100W, 100-240V, 50/60Hz |
![]() |
Liquid handling |
Integrated Autosampler
|
|
Integrated Syringe pump
|
||
|
Integrated Degasser
|
||
|
Dimensions |
62 x 41 x 47 cm (24.5" x 16" x 18.5") |
|
|
Weight |
|
|
Liquid handling | Integrated 2-channel peristaltic pump, software controlled |
| Sample loading and injection | 12-port injector | |
| Syringe pump Optional |
2-channel, flowrates 0.831 nL/min - 150.5mL/min, syringe sizes 10 L - 140 mL | |
| Autosampler Optional |
|
|
|
Degasser |
2-channel degasser, internal volume only 480 µl, eliminates baseline fluctuations, advanced error and leak checking functions, wetted parts: PEEK and Systec AF | |
| Dimensions | 38 x 32 x 31 cm (15" x 12.5" x 12.4") | |
| Weight | 10kg (22 lbs) |
1 µRIU (1 x 10-6 of Refractive Index Unit, a physical measure) corresponds to 1 RU (Resonance Unit, an arbitrary unit used by competitors) based on (1)
References:
(1) R.B.M. Schasfoort and A.J. Tudos, Handbook of Surface Plasmon Resonance, RSC Publishing, ISBN 978-0-85404-267-8, pp. 44